| dc.description.abstract | Microhyla fissipes is a species which can be easily found in Taiwan, China and Indo-China. Boulenger in 1884 reported Microhyla fissipes as a new species with type locality in Tainan, Taiwan, but not well be recognized until 2005. Besides,there was a new species named Microhyla mukhlesuri published in 2014 and its distribution was considered greatly overlapping with Microhyla fissipes’s. In addition, the phylogentic relationship of Microhyla fissipes and Microhyla mukhlesuri in East and Southeast Asia is imperative to be clarified. In this study, we used the DNA sequences of 12S,16S and Cytb acquired from 69 samples newly collected in Taiwan with published data on NCBI to investigate the phylogeoegaphy of Microhyla fissipes in East and Southeast Asia by p-distance ,the Bayesian inference ,Maximum Likelihood and Molecular clock estimates. Based on our results, we summarized that there were four groups in East and Southeast Asia, including Taiwan-China group, Mid-Vietnam-East-Thailand group, South Vietnam group,and West Thailand group. According to the species type locality, Taiwan- China group is Microhyla fissipes and West-Thailand group is Microhyla mukhlesuri. In addition, the group in South Vietnam and the group of Mid-Vietnamm-East-Thailand might be two cryptic species in Indo-China. The ancestors of these four groups started to diverse about 24,600 years ago during the latest glacial epoch period. | en_US |