| dc.description.abstract | Rice is an important crop that provides food for nearly 50% of the world’s population. Although advanced technologies have been developed to enhance rice yield, increasing rice yield still is the main goal for the researcher. Nutrition use efficiency of rice is essential for high yield. To identify factors involving in the nutrition utilization process, microarray analyses were performed in rice grown under nitrogen (N), phosphate (P) and carbon (C) starvation conditions. A total of 32 genes with altered mRNA accumulation under N, P, C- starvation conditions were selected for further study. A total of 69 T-DNA insertion mutants corresponding to those genes were collected from the Taiwan Rice Insertional Mutant (TRIM) population for further investigation. After screening, 38 T2 generation mutant lines were grown in the rice paddy for the examination of phenotypes and propagation of T3 seeds. Numerous mutant lines showed phenotypes such as late maturation, dwarf, yellow leaf, programmed cell death, erect leaf reduced-growth, higher grain yield, and lower grain yield. Genotyping has been carried out to determine whether mutant phenotype correlates with the T-DNA insertion on the target genes. Erect leaf and yellow leaf mutants were grown in the greenhouse and in the rice paddy to confirm the correlation of phenotype and genotype. Expression of genes surrounding mutant showing yellow leaf and erect leaf were determined using the RT-PCR analysis. Two genes, LOC_Os03g53630 and LOC_Os03g53650 with increased expression in the yellow leaf mutant, were isolated to generate overexpression lines. The expression of LOC_Os03g53640 was found to be decreased in the homozygous plant, which is correlated with the recessive yellow leaf mutant phenotype. LOC_Os03g53640 is a homolog of Photosystem II 11kDa protein found in Cyanobacteria and Low PSII accumulation19 found in Arabidopsis. Knock-out of LOC_Os03g53640 will be performed using the CRISPR/Cas 9 technology in the future. | en_US |