| dc.description.abstract | Lung cancer has a high mortality rate and a very low cure rate. The most important one is that the Metastasis rate is very high, and both radiotherapy and chemotherapy have many side effects. Currently, it is often used for the Target treatment of lung cancer. It produces strong resistance, so we hope to find new treatments, and many literatures show that natural medicines can be used to treat cancer, with lower toxicity and lower side effects. After drug screening, it was found that Traditional Chinese Medicine BP010W can inhibit the migration ability of lung cancer cells., thus was selected for further research. The column extraction was performed for isolation of prue compounds from BP010W using different concentrations of ethanol, 0%, 20%, 50% and 95%, and we found the concentrations of 20% and 50% have most effective in inhibiting migration of cell. We assay the bioinformatics and literature references, and It is concluded that the estrogen pathway affects the regulation of lung cancer. Therefore, we screened out the steroid alkaloid Solasodine in Chinese medicine BP010W. HPLC analysis showed that Solasodine appeared in our BP010W 20% and BP010W 50% isolates. It was also confirmed by dosing experiments that Solasodine inhibited the migration ability of lung cancer cells, and then used Western blotting to prove that the migration-related proteins were inhibited. Finally, we using the wound-Healing assay to prove that estrogen will increase the migration ability of lung cancer cells, we also treated Solasodine and estrogen together, and found that each other can compete with each other. | en_US |