| dc.description.abstract | In recent years, due to the number of people who are ill with cancer grow up, many people have studied the difference between cancer cells and normal cells. The differences between cancer cells and normal cells include cell shape, cell size, etc. In avoid to damaging the sample and the position of the nucleus can be clearly distinguished, the position of the nucleus is located by the difference in refractive index between the nucleus and the cytoplasm. At present, most of the instruments for detecting the whole field refractive index are scanning interferometers, and they are susceptible to environmental interference during the detection. It takes a while to obtain the refractive index. This paper will propose a method to solve the environmental interference and quickly obtain the refractive index.
In this experiment, the Linnik-type polarization interferometer combined with the camera produced by 4D Technology Company is used as the experimental framework to effectively reduce the environmental interference, and the biological measurement can be effectively measured even when the optical table is not inflated. In order to test the feasibility of the experimental instrument, the optical film was first to be analyzed. The thickness error was 0.03%, the standard deviation was 0.74 nm, the refractive index error was 0.24%, and the standard deviation was 0.0032.
The recognized cytoplasmic refractive index ranges from 1.35 to 1.37, and the microbial cytoplasmic refractive index detected in this experiment is within its range. In order to achieve the instantaneous detection of the change of refractive index, this experiment uses the algorithm to obtain the thickness of the microorganism, and calculates the phase modulation level. By inputting the phase modulation series into the program, the refractive index and thickness of the whole living microorganism can be calculated instantly.
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