| dc.description.abstract | Previous reports have demonstrated that exposure of electromagnetic force could affect
cellular physiology. Others have shown that static magnetic field (SMF) has impacts on cell
proliferation, particularly in cancer cells. Several physical parameters, such as magnetic
frequency, gradient, and magnitude, were reported to affect the biological consequences.
However, discrepancies exist between the SMF and cancer cell responses and the mechanisms
underlying the SMF-mediated effects remained largely unexplored. The main purpose of this
study is to investigate the mechanisms of SMF-mediated effects in cancer cells. Our results
showed that the exposure of SMF affected cancer cell proliferation and cell cycle distribution.
The doubling time for cells exposed to SMF was longer than that of control group. The results
of flow cytometry showed that SMF induced higher percentage of cells accumulated in the
mitotic phase compared to that of control group after 24-hour exposures (% of G2/M，SMF：
control= 5.50 : 0.25). Results of immune-fluorescent staining and western blotting found
higher expressions of cyclin B1 and cyclin E1 SMF-treated cells. Furthermore, the activation
of ATM-NBS1-CHK signaling pathway was enhanced. The results of Next Generation
Sequencing (NGS) analysis showed that the SMF primarily regulated genes involved in
functions of motility, immune and embryonic development related pathways. From the
time-lapse fluorescent microscope observations, more cancer cells exposed to SMF were
accumulated in mitotic phase. The SMF-treated cells exhibited a shrinkage phenotype, faster
motion frequency, and shorter peripheral protrusions. These observations indicate that the
v
SMF may affect polymerizations of microtubules and F-actin, as well as cell adhesion. To test
the SMF-mediated effects on development, we used zebrafish as the model and evaluated the
phenotypic alterations during development. We found that SMF exposures evidently affected
the distribution of melanin. Some embryonic malformations were also observed under SMF
treatment. However, there’s no difference on the survival ratio between SMF-treated group
and control group. The presented study helps to understand more molecular mechanisms of
the SMF-mediated effects on cancer cells. This may provide an opportunity to utilize the
features of SMF to tailor therapeutic strategy. | en_US |