| dc.description.abstract | Type II diabetes mellitus (T2DM) is the most common form of diabetes,
accounting for 90-95% of diabetics, and is a chronic disease often preceded by a
long asymptomatic period. Most of T2 diabetics suffer with delay wound healing
resulted from poor blood circulation, prolonged inflammation and lack of growth
signal due to disordered metabolism, and it may severely affect patients’ life
quality and even cause death. To overcome the aforementioned issues, a synthetic
chitosan-based hydrogel containing silver ions and growth factor-entrapped
nanoparticles that may provide both antibacterial and would healing-enhanced
functionalities were developed in this study. As being the preliminary
investigation, the bovine serum albumin (BSA) was employed as the model
protein molecule to substitute the practical growth factor protein in this study.
Furthermore, the BSA-encapsulated chitosan nanoparticles were fabricated by
using the polyanion tripolyphosphate as the coacervation crosslink agent.
Through the DLS analysis, the size and surface charge of the BSA-loaded
nanoparticles(CTBNPs) were 265 ± 26 nm and 30.02 ± 1.42 mV, respectively.
The encapsulation efficiency and the loading rate of the BSA in the nanoparticles
were 74± 8.62% and 1.23 ± 0.55%, respectively. Also, the release rate of BSA
from the nanoparticles was 89% within 4 h, while that from the synthetic hydrogel
was about 70% within the same time course. The nanoparticles were successful
insides the chitosan hydrogel. From hydrogel property test, BSA releases were
70% . When the silver ions released in 48 hours, the 4 and 37 degrees release were
5.11 and 6.53 mg/L. Moreover, the bacterial inhibit study, when used 24 mM
silver ions concentration the bacterial inhibit was significant. The hydrogel
toxicity for the cell. In the cell study, we used 24 mM silver ions concentration
the cell viability came to 90 %. In the growth curve study, we decide used
epidermal growth factor with different concentration. However, when EGF came
to 60 μg/mL cell weren′t affected by silver ions. We decided that the final
formulation of the hydrogel was 60 μg/mL EGF and 24 mM silver ion
concentration.In animal experiments, We used Sprague Dawley (SD) rats and
injected with streptozotocin to observe wound healing on rats′ backs after 2 weeks
of diagnosis as diabetic rats and to observe wound healing using dressings. The
results showed that the wound healing rate of CPEHG water gel in each group
was accelerated, inflammatory cell implantation and complication of collagen
deposition, which could be used to treat diabetic foot wound dressing in the future | en_US |