| dc.description.abstract | This thesis aims to discuss the process and control of bioreactor based on low cost and easy preparation. In cell-culture, we often use Lab-on-chip to culture cells in vitro. Most bioreactor research has focused on small-scale observations, but has abandoned the possibility of mass-culturing cells. At the same time, a lot of microfabrication processes and the circuit control required to simulate biological activities also need device preparation ability of biological experiment operators. Therefore, how to make the experiment operators easy to make is still a problem. Most scholars usually do not focus on low cost and reduce the experimenter′s manufacturing ability, so this paper will discuss this aspect.
In this thesis, we improved a bioreactor for mechanical and electrical stimulation, so that it can allow users to produce and assemble it easily, and then they can easily learn and adjust parameters to experiment flexibly. We use a CNC milling machine and a drilling machine to prepare the flow channel layer, and a film made of PPy coating PDMS material provides conductivity to electrically stimulate cells. Then the film is bonded on the fluid layer, and the stretching is provided by pump to mechanically stimulate the cells. At the same time, we made a shell with electrodes to fix the whole device to avoid liquid leak and replace the function of traditional culture dishes. In addition, there is a control box for electromechanical control. The control box will be used to control the pump with an electromagnetic valve to adjust the mechanical stretch to stimulate the cells, and to use a pulse signal made by SSR (solid-state-relay) to electrical stimulate the cells. Moreover, the complex stimulation also confirmed that the two can help each other and gain greater benefits. The digital control of the device also allows they to adjust their own parameters needed, and it also remains device’s expandability in the future. | en_US |