| dc.description.abstract | In recent years, the Environmental Protection Agency and the Committee of Agriculture have vigorously promoted the policy of resource utilization of livestock manure and urine, suggesting that livestock farmers can no longer go through the previous three-stage treatment, but only need to go through the general anaerobic digestion process to convert the livestock excrement produced by the pasture. It is used as biogas residue and applied to agricultural land as fertilizer, so that livestock manure that should have been discharged into receiving water bodies and may cause pollution to spread can be used for ＂farmland agriculture＂, echoing the current ＂circular economy＂ policy. However, the existing literature has pointed out that the traditional anaerobic digestion treatment at room temperature cannot effectively reduce the concentration of antibiotic resistance genes (ARG) contained in livestock manure and urine, and the consequence of the continuous increase of ARG concentration in the environment has been It is predicted by international authoritative organizations that it will seriously threaten the achievements of modern medicine. To some extent, it implies that if we want to promote the policy of using biogas residue as agricultural land fertilizer, we should conduct a careful investigation to confirm whether the drug resistance of the soil environment will be derived from its growth issues. Because the concentration of ARG has not yet been regulated by regulations, this study conducted a survey on the content of ARG in the long-established ＂compost＂, to provide a reference and comparative value for the risk assessment of environmental antibiotic resistance caused by biogas slurry and residue.
The 28 compost samples selected in this study covered different raw materials such as non-poultry animal manure, cow manure, pig manure, chicken manure, etc. The target genes (including various ARGs, mobile genetic elements, and 16S rRNA genes) in the samples were quantified by real-time gene quantitative technology, and statistically analyzed the correlation and difference between various samples. The survey results showed that: (1) the content of antibiotic resistance genes in the manure compost was higher than that of the non-manure compost; (2) both non-manure and manure compost mainly contained sulfonamides antibiotic resistance genes and intI1; (3) heavy metals zinc and copper in compost are negatively correlated with antibiotic resistance genes; (4) the residual antibiotics in compost and antibiotic resistance genes are closely related. (5) When the compost enters the soil environment, it is mainly composed of tetracycline and sulfonamide antibiotic resistance genes and intI1; (6) The antibiotic resistance genes and intI1 in compost After entering the soil environment, its fate in the environment will not vary due to different sources. These results indicate that the abundance of antibiotic resistance genes and intI1 in composts from different feedstock are indeed different, but their impact on the entry of antibiotic resistance genes into the environment will not be affected by different raw materials. difference. In addition, the degradation rates of antibiotic resistance genes and intI1 in compost in the environment deserve to be tracked and confirmed. | en_US |