| dc.description.abstract | The optical microscope, which enabled people to observe invisible objectives more details such as surface materials, cells, or microorganisms played an important role in various fields of engineering, physical science, medicine, and biology. However, the application was limited by the complexity and expensive cost of the current optical imaging platform.
The Fresnel Digital Holography (FDH) proposed in this study utilized scalar diffraction theory to simplify optical imaging equipment. It no longer needed large and complicated optical components were composed of non-coherent light, pinhole, and image sensor. Without the lens to limit the field of view (FOV), the high spatial resolution close to the diffraction limit could be achieved on a large FOV (30mm2). By controlling the spatial coherence of the light source, the diffraction pattern was recorded on the sensor, and the image with the same resolution as the 20x microscope was reconstructed by the inverse Fourier transform without any optical lens within 5 seconds. The accuracy of red blood cell count is 93.5%, and that of white blood cell is about 91%. The system will not only bring the innovative breakthroughs in biology and medical diagnosis, but also enhance the possibility of early prevention and early diagnosis, especially in areas where the medical resources are scarce.
In this study, the complete blood cell analysis was used for practical application. After matching with the micro-channel blood sampling chip by self-designed, the empirical evidence obtained from in the experimental results proved that the system could perform rapid, high-throughput blood cell characterization analysis and the function of complete blood count with the trace blood sample. The system provided a portable and economical alternative of the contemporary whole blood analysis tools. | en_US |