| dc.description.abstract | Metal-organic frameworks (MOFs) have become one of the most popular porous crystalline materials in recent years. Because of their porous diversity, large specific surface area and thermal stability, they are promising for a wide range of applications, for example enzyme immobilization is one of the attractive areas for our interests. In 2015, our group encapsulated catalase (CAT) enzyme into Zeolitic imidazole framework-90, CAT@ZIF-90, via mild water-based de novo approach, and afterward encapsulated enzymes into MOFs, Enzyme@MOFs, via a mechanochemical method in 2019. One of the mainly above-mentioned enzymes is catalase, which has the characteristics of a small substrate and no need for the coenzyme, but it is easier to lose activity in an alkaline environment. In order to expand the applications of de novo approach, we also encapsulated urease, which has a relatively lower reaction rate that provides a new platform for observing enzyme catalysis. In summary, we successfully synthesized Enzyme@ZIF-90 via a mechanochemical method and identified the characteristics through various instruments. Although urease did not show the expected activity, this method still retains catalase activity and improves the yield. Therefore, it still has a great potential for the further industrial applications. | en_US |