| dc.description.abstract | The definition of chronic wound is that the wound cannot achieve structural and external healing after proper treatment. When the lesion is exposed, Staphylococcus aureus, the main lesion on human skin, proliferates in large quantities, slowing the recovery of the skin wound. Dressings containing silver ions are the mainstream of the development of dressings for chronic wounds, but in recent years, many studies have pointed out that silver ions have a certain degree of toxic damage to cells. Silver ions are not the only option for antibacterial dressings, and the use of antibiotics is also restricted due to drug resistance issues. However, polyhexamethylene biguanide (PHMB) has been proven not only to have low cytotoxicity to skin cells, but also to have a good inhibitory effect on bacteria. In wound healing, epidermal growth factor (EGF) stimulates the proliferation of human keratinocytes. In this study, a dressing synthesized by the freeze-thaw method was developed, and a high-strength chitosan/PVA hydrogel containing PHMB and EGF nanoparticles was prepared through the chemical polymerization and physical interaction of chitosan. Compared with the carrier-free group, EGF-nanoparticles prolonged the release time of EGF. As the concentration of EGF increased, it had a significant positive effect on the growth of keratinocytes. According to the literature, oxygen is helpful for the formation of microvessels on the wound, so this study also added PFOB nanoparticles to the hydrogel to make the hydrogel have the ability to carry oxygen. Our research shows physical analysis, mechanical properties, antibacterial, anti-inflammatory and oxygen carrying capacity. As the main dressing material, this hydrogel creates a moist environment that helps wound repair. It not only has a good antibacterial effect, but also has the ability to promote the growth of lesion cells and provides more than normal oxygen content. To promote microangiogenesis. In the future, we expect that this hydrogel can significantly improve the therapeutic effect of chronic wound healing. | en_US |