dc.description.abstract | Due to the high selectivity of peptides, the uniqueness and variability that can be composed of different amino acids, and the advantages of easier production compared with proteins, the development and application of peptides have recently received great attention from scientists. Here, this study provides a method different from the previous synthetic methods of peptides and polymers and provides two peptide-polymer conjugates for bioactive polymeric materials. In the first part, solid phase peptide synthesis was applied for synthesizing acrylic peptides composed of Lysine acrylamide and conventional amino acids, as a crosslinker for hydrogels, then use nuclear magnetic resonance (NMR) and mass spectrometry (MS) to identify its chemical structure. The peptide can be cleaved when treating with trypsin, and the hydrogel with enzymatic degradation has been developed. Compared with the hydrogel formed by N,N′-Methylenebisacrylamide crosslinker, we found that after soaking in trypsin, only the acrylic peptide hydrogel disintegrated. The applications in biomimetic polymers such as biodegradable scaffolds and drug delivery has great potential. The second part of the work is to prepare acrylic peptides through 6-Acrylamidohexanoic Acid and combine them with the Integrin recognition ligand of arginine-glycine-aspartic acid (RGD). RGD peptide is a cell adhesion sequence because it is easy to interact with integrins. Here, the hydrogel with acrylic peptide-RGD ligand and general hydrogel are co-cultured with cells in DMEM. Observing the surface of the hydrogel with the optical microscope, the results presented that the hydrogel with acrylic peptide-RGD ligands has cell adhesion. Different from general hydrogels, the addition of acrylic peptide-RGD ligand can support cell growth with materials that are not conducive to cell growth, and closer to the conditions in vivo, this can be applied to the functional biomaterials for tissue repair. Finally, FT-IR structure identification and XPS surface element analysis were used to confirm the polymerization of acrylic peptide-RGD ligand and hydrogel, and through the polymerization of acrylic peptide-RGD ligand and fluorescent molecules into linear fluorescent polymers to label cells, confirming that acrylic peptide- RGD ligand and polymer can still interact with cells when they form a linear polymer. This research has developed peptide-polymer conjugates and their synthesis strategies. It is hoped that this can be applied to medical treatment and provide new biomaterial design methods. | en_US |