| dc.description.abstract | Low temperature adversely affects plant growth and crop production. Correct RNA structure is difficult to be maintained under low temperature conditions. RNA helicases play an important role in maintaining functional RNA structures. DEAD-box RNA helicases belong to superfamily II of the RNA helicase family, and their function are involved in many cellular processes, including transcription, RNA splicing, ribosomal RNA biosynthesis, RNA export, translation, etc. Previous study showed that the OsRH42 is involved in rice growth and cold-responsive genes pre-mRNA splicing. In this study, a OsRH42 mutant line was generated by using a CRISPR-Cas9 system. T0 generation of OsRH42 mutant occured fragment deletion mutations at coding region of OsRH42 gene at both chromosomes, one chromosome has 60 bp deletion (in-frame mutation) and another chromosome has 11 bp deletion (frameshift mutation). T0 generation mutant had 25% seed setting rate. Interestingly, T1 generation seeds all were homozygous of 60 bp deletion in-frame mutation. The T2 generation in-frame mutation mutant showed shorter plant height and reduced cold tolerance, compared with wild type. These results showed OsRH42 defect by frameshift mutation leaded to seed abortion, implied that OsRH42 is an essential gene during develpoment of male and female gametes. Besides, T2 generation in-frame mutant slightly increased the intron retention of cold stress-responsive genes after 18 h cold stress. Thus, the T2 generation in-frame mutant showed adversely affect rice growth, reduced the intron splicing efficiency of cold stress-responsive genes, and ultimately led to reduced cold tolerance in rice. | en_US |