| dc.description.abstract | With the increasing prevalence of cancer and various chronic diseases, the demand for drug testing and precise drug delivery has been steadily rising, leading to the development of three-dimensional cell culture techniques. Currently, common three-dimensional cell culture techniques include scaffold-based approaches (such as hydrogels and porous solid scaffolds) and scaffold-free approaches (such as hanging drop culture and low-attachment plates). The commonly used commercial options include Matrigel and Ultra-low culture plates, while in laboratory research, many natural or synthetic polymers have been used as materials for three-dimensional cell culture. In this paper, we utilized a material called Kollicaot@IR, which consists of polyvinyl alcohol (PVA) and polyethylene glycol (PEG), as the surface material. We connected a photocurable functional group, 4-azidobenzoic acid, to the hydroxyl groups (-OH) of polyvinyl alcohol. By irradiating it with ultraviolet light at a wavelength of 365nm, nitrogen gas (N2) is removed, allowing the photocurable functional group to be immobilized on the cell culture plate. This light-curable copolymer material was applied to the Rapid, Reproducible, Rare Cell 3D Expansion (R3CE) cell culture technique to investigate its impact on the R3CE platform and whether it offers advantages over the original R3CE platform. HCT-116 cells were used in the experiments, and it was observed that the cells still formed spheres, with the size of the spherical cells controlled at approximately 100 μm, facilitating subsequent analysis in drug testing experiments. | en_US |