| dc.description.abstract | Nowadays, sweeteners are widely used. High fructose corn syrup (HFCS) is a common sweetener in various food industries. HFCS can imparts sweetness, enhances taste, and increase the moisture content of food. With the easing of the COVID-19 pandemic as well as the recovery of commercial activities, global market for HFCS is projected to grow at a rate of 3-5%, annually. According to reports, it is expected to reach billions of US dollars by 2030.
In order to reduce the cost of enzymes used in the production of HFCS, enzyme immobilization techniques are employed to enable enzyme recycle, reusability, and lower production costs. However, there are still some challenges for imbedding glucose related enzymes into supporting materials because of complicated procedures and significant expenses.
The recipe used in this study is based on the methods in previous papers published by our laboratory (JACS 2015 and ACS AMI 2021). In this study, we encapsulated two enzymes, invertase and glucose isomerase, which are essential to produce HFCS, into metal-organic frameworks (MOFs) called Zn-MOF-74 under de novo mild water-based condition. The de novo approach is a method which is able to rapid and simple encapsulation of enzymes into MOFs material under mild water solution at room temperature. The aims are to directly convert sucrose into HFCS through a tandem reaction. This approach enables the rapid separation, recovery, and reactivity of enzymes and products using simple filtration.
We successfully encapsulate invertase and glucose isomerase into Zn-MOF-74 material for obtaining enzymes@MOFs biocomposites using the de novo approach. After immobilization, the activity of GI@Zn-MOF-74 was maintained 80% of its non-immobilized one. Moreover, the immobilized invertase not only retained its activity (kobs=9.1*10-2) but also exhibited significantly higher activity compared to the non-immobilized invertase (kobs=1.2*10-3), even under unfavorable reaction conditions. This study demonstrates an enzyme immobilization approach for creating enzymes@MOFs biocomposites with simplified process, which could lead to a more cost-effective production of HFCS. Additionally, it was discovered that immobilized enzymes have the potential to adapt broader pH range in their applications, expanding their usability in various fields. | en_US |