dc.description.abstract | Triton X-100 is a non-ionic detergent and it often used in industrial, agricultural and household area. There are 5~85% surfactants mixing in the pesticides and herbicides. It can be directly introduced into the environment by spraying the pesticides as well as the herbicides through agricultural activities. The structure of the surfactant includes both hydrophilic and hydrophobic group. In addition to the toxicity, its chemical property also has great influence on the organic contaminants fates in the soil and even impacts public health. The focal point in this study is to use 16S ribosomal DNA fingerprinting method and denaturing gradient gel electrophoresis (DGGE) to analyze the community in the surfactant-polluted soil. Besides monitoring the relationship of community and Triton X-100, we added Pseudomonas sp. SH4 to see what changed in the community and Triton X-100 biodegradation. In this study, we used the microcosm which contained surfactant-like polluted soil over a long period of time to proceed with the research of surfactant bioremediation. There were 4.35 g Triton X–100 degrading in the SH4-added groups (72.5% of the original Triton X-100 weight). We chose the sample to proceed with 16S rDNA cloning library. Then we identified 11 strains by DGGE screening. From the phylogenetic analysis we knew that they were belong to the α, β, γ-proteobacteria. (except of the Flavobacterium sp. wuba 46) In the DGGE fingerprinting we found that Pseudomonas sp. SH4, Stenotrophomonas maltophilis, and Agrobacterium tumefaciens Zutra F/1 were dominant strains in the third group. Also, Pseudomonas sp. SH4, Stenotrophomonas maltophilia, Stenotrophomonas sp., and Stenotrophomonas maltophilia were dominant in the fourth, fifth, and sixth groups. In the group with SH4 and Triton X-100 but without air, we found that Agrobacterium tumefaciens Zutra F/1 and clone 4-70 (uncultured beta proteobacterium) are also dominant strains. Two microcosms contained Pseudomonas sp. SH4 and kept pumping air, but one of them was 30℃ fixed controlled. Two months later, 6 g Triton-100 were degraded completely. Whereas, the groups without SH4 degrader still contained about 2.67 g Trioton-100. Even three months later, 1.45 g Triton-100 would still be detected. As a result, Pseudomonas sp. SH4 plays an important role in the bioremediation. | en_US |