| dc.description.abstract | Mushrooms have been used for many years in oriental culture as tea and nutritional food and because of their special fragrance and texture. There biochemical composition, with significant content of protein, carbohydrate, lipids, enzymes, minerals, vitamins and water, has attracted attention also as a functional health food. The mushroom Agaricus blazei Murill, popularly known as ‘Cogumelo do Sol’ is originally native from Brazil in Piedade, state of S. Paulo. Its anti-tumor and anti-carcinogenic activities have been considered and extensive research has been carried out to elucidate the polysaccharide structure and its relationship to the anti-tumor activity.
In order to increase of the polysaccharides of Agaricus blazei and bioactivity, this research carries on the discussion with environmental factors that ferment in many aspects, and the bioactivity influence of living beings on polysaccharides. This experiment research results are divided into four the following parts and discussed mainly.
First part is the sumbuerged culture experiment and looked for the rightist Agaricus blazei to ferment in the shaker.
Second part is hydrolyase of ferment activation to discuses.
Third part is production of endo-1,3-β-glucanase from the fungus Agaricus blazei in air-life fermentor.
Fourth part, observe the relation of the bioactivity which hydrolysis ferments and polysaccharides.
The experimental result shows, the sugar compositions of polysaccharides from Agaricus blazei fruit body is glucose mainly, the sugar compositions of polysaccharides of fermentation broths is mainly on mannose, the exopolysaccharides of Agaricus blazei contain fucose for a few fungi mushroom. Optimization of submerged culture process for the production of biomass, exopolysaccharides and bioactivities by Agaricus blazei: carbon source- glucose、fructose；C/N-12.73；phosphate-0.2～0.3 %；vitamin B1-0.001～0.003 %. | en_US |