| DC 欄位 |
值 |
語言 |
| DC.contributor | 生命科學系 | zh_TW |
| DC.creator | 蒲欣儀 | zh_TW |
| DC.creator | Shin-Yi Pu | en_US |
| dc.date.accessioned | 2004-7-15T07:39:07Z | |
| dc.date.available | 2004-7-15T07:39:07Z | |
| dc.date.issued | 2004 | |
| dc.identifier.uri | http://ir.lib.ncu.edu.tw:88/thesis/view_etd.asp?URN=91224002 | |
| dc.contributor.department | 生命科學系 | zh_TW |
| DC.description | 國立中央大學 | zh_TW |
| DC.description | National Central University | en_US |
| dc.description.abstract | 中文摘要
為了了解植物耐受高溫的遺傳控制因子及其生理機制，我們用化學突
變劑( EMS )誘導阿拉伯芥點突變產生，並進而篩選出一株對熱缺乏耐受
性之突變種，稱之為hit1( heat intolerance ) 。這是一種典型從具遺傳性之
生理性狀找出相對應之基因及其分子作用的研究方式，亦即所謂的向前式
遺傳學分析法( forward genetics )，為功能性遺傳學研究法中的一種。因
hit1 之性狀為點突變所造成的，故其功能性基因須以遺傳圖譜為基礎來進
行定位選殖( map-based cloning, MBC ) 。其做法是先將Columbia 生態
型之hit1 突變種與Landsberg 生態型之野生種雜交，之後的F1 自交得F2
世代，F2 世代中帶有hit1 性狀之個體為hit1 定位時所需之分離族群
( segregation population )。以hit1 之突變性狀，37℃處理四天後會死亡的
原則篩選， 再經簡單序列長度多型性( simple sequence length
polymorphism , SSLP )及限制酵素切割擴增片段多型性( cleaved amplified
polymorphic sequence , CAPS )兩種對生態型具專一性之分子標記進行定
位。本研究共設計了26 組SSLP 分子標記，7 組CAPS 分子標記。在檢
測過3987 株F2 世代的hit1 突變種之後，推得hit1 基因坐落在第一條染色
體， AGI map 18767.3 kb ~ 18810.2 kb 之42.9kb 範圍內，達到MBC 所需
之定位目標。 | zh_TW |
| dc.description.abstract | Abstract
In order to understand the mechanism of plant crop heat stress, we
screened a mutant that is difficult to tolerant heat stress called “hit1”
(heat intolerance) inducible by chemical mutagenesis, EMS, causing a
point mutation. Forward genetic is one of functional genetics approaches,
and it is underlying a mutant with a desirable genetic phenotype to an
identified mutation in a gene and to analysis specific gene’s molecular
biology function. We use forward genetic to approach “hit1” that was a
point mutation, so we want a cloning of sequencing mutant-define gene
by map-based coloning (MBC). A hit1 mutant with Columbia ecotype (P1)
and a wild type with Landsberg erect ecotype (P2) were parent to get
recombinant inbred lines (F2). It has hit1 mutant specific phenotype lines
of F2 generation that is a segregation population to provide hit1 genetic
mapping. The definition of hit1 mutant phenotype was death at 37℃ in 4
days to screen mutants from F2 generation as a segregation population
then make hit1 gene genetic mapping, and we designed 26 pairs primers
of simple sequence length polymorphism (SSLP) markers and 7 pairs
primers of cleavage amplify polymorphisms (CAPS) markers for genetic
analysis. We suggest that hit1 gene locus in AGI 18767.3kb ~ 18810.2kb,
total 42.9kb regions, and approach the fine-scale mapping of MBC. | en_US |
| DC.subject | 阿拉伯芥 | zh_TW |
| DC.subject | 對熱不耐受性突變株 | zh_TW |
| DC.subject | hit1 | en_US |
| DC.subject | heat intolerance | en_US |
| DC.subject | Arabidopsis | en_US |
| DC.title | 阿拉伯芥突變種(hit1)之位址定位 | zh_TW |
| dc.language.iso | zh-TW | zh-TW |
| DC.title | Genetic Mapping of hit1 Locus in Arabidopsis thaliana | en_US |
| DC.type | 博碩士論文 | zh_TW |
| DC.type | thesis | en_US |
| DC.publisher | National Central University | en_US |