| DC 欄位 |
值 |
語言 |
| DC.contributor | 物理學系 | zh_TW |
| DC.creator | 何愛玲 | zh_TW |
| DC.creator | Ai-ling Ho | en_US |
| dc.date.accessioned | 2008-7-24T07:39:07Z | |
| dc.date.available | 2008-7-24T07:39:07Z | |
| dc.date.issued | 2008 | |
| dc.identifier.uri | http://ir.lib.ncu.edu.tw:88/thesis/view_etd.asp?URN=942202026 | |
| dc.contributor.department | 物理學系 | zh_TW |
| DC.description | 國立中央大學 | zh_TW |
| DC.description | National Central University | en_US |
| dc.description.abstract | 我們的研究分為兩部分：(I)兩種成分的細胞膜：POPC、POPE；(II)三種成分的細胞膜：POPC、POPE、sterol。利用2H-NMR和DSC，測得POPC/POPE細胞膜的相行為會隨著POPE濃度及溫度而改變。在2H-NMR中，我們以氘取代POPC及POPE sn-1鏈上的氫，來量測POPC及POPE的行為。我們是第一位提出POPE濃度從0到60mol%對溫度的相圖。在相圖上發現一個大範圍的膠態(gel-phase)及液晶態(lc-phase)之兩態共存區。且發現在低於十七度時，POPC及POPE並不是混合得相當均勻，只有在超過十七度時，才有較均勻的混合。
利用POPC及POPE上的氘，2H-NMR測得POPC/POPE細胞膜出現固醇類時(1:1:1 POPC/POPE/ergosterol及1:1:1 POPC/POPE/cholesterol) 的相行為會隨著POPE濃度及溫度而改變。實驗中發現，這兩種固醇，在膠態會降低POPC/POPE (1:1)膜的有序程度，但在液晶態卻能增加POPC/POPE (1:1)膜的有序程度，且麥角酯醇的效應低於膽固醇。在沒有固醇類的情況下，POPC及POPE只能在某些溫度下混合均勻，而混合不均勻的溫度區域，在加入麥角酯醇及膽固醇後，有助於POPC及POPE的混合。此外，對於麥角酯醇幫助POPC/POPE膜均勻混合的能力而言，其作用的溫度範圍明顯小於膽固醇。 | zh_TW |
| dc.description.abstract | We investigate two-component membranes composed of POPE and POPC, and three-component membranes composed of POPE, POPC and sterol. The phase behavior of POPC/POPE membranes was studied as a function of POPE concentration and temperature by 2H-NMR and DSC. For 2H-NMR measurements, POPC and POPE were deuterium labeled alternatively such that the behavior of both POPC and POPE can be observed. We present the first temperature-composition diagram for POPE concentration range from 0 to 60 mol%. Coexistence of the gel and lc phases is observed over a wide temperature and composition range. It is found that POPC and POPE do not mix well in 1:1 POPC/POPE below17℃, but mix well above 17℃.
To investigate the properties of POPC/POPE membranes in the presence of sterol, 1:1:1 POPC/POPE/ergosterol and 1:1:1 POPC/POPE/cholesterol were studied as a function of temperature by 2H-NMR, with POPC and POPE deuterium labeled alternatively. We show that the presence of ergosterol (or cholesterol) disorders gel-phase POPC/POPE (1:1) membranes, whereas orders lc-phase POPC /POPE (1:1) membranes. The effect of ergosterol is weaker than cholesterol. Furthermore, the presence of ergosterol (or cholesterol) modulates the mixing properties of POPC/POPE membranes. POPC and POPE mix well in some temperature range, where they are poorly mixed in the absence of sterol. The enhancement of mixing of of POPC/POPE membranes by ergosterol is observed in a temperature range narrower than that by cholesterol. | en_US |
| DC.subject | 膽固醇 | zh_TW |
| DC.subject | 氘核磁共振 | zh_TW |
| DC.subject | 示差掃描量熱儀 | zh_TW |
| DC.subject | 麥角酯醇 | zh_TW |
| DC.subject | cholesterol | en_US |
| DC.subject | ergosterol | en_US |
| DC.subject | POPC | en_US |
| DC.subject | POPE | en_US |
| DC.subject | phospholipid | en_US |
| DC.subject | sterol | en_US |
| DC.subject | 2H-NMR | en_US |
| DC.subject | DSC | en_US |
| DC.title | 模型細胞膜(含有相同碳鏈的PC/PE)存在或缺乏固醇類的物理性質 | zh_TW |
| dc.language.iso | zh-TW | zh-TW |
| DC.title | The Physical Properties of Model Membranes Composed of PC and PE with Identical Acyl Chain in the Presence and Absence of Sterol. | en_US |
| DC.type | 博碩士論文 | zh_TW |
| DC.type | thesis | en_US |
| DC.publisher | National Central University | en_US |