|DC.description||National Central University||en_US|
|dc.description.abstract||Stem cell separation through membrane filtration method is simple and inexpensive, and sterility is easy to maintain during the process. The recovery ratio of HSCs from umbilical cord blood (UCB) was found to be 7-20% depending on the recovery solution. A high recovery ratio of HSCs was obtained when 20 wt% of dextran was used as the recovery solution. Direct ex vivo expansion of hematopoietic stem cells (HSCs) from UCB by membrane filtration method has been developed in this study. This study also reports the expansion ratio and ability of colony-forming units of HSCs from UCB purified by conventional Ficoll-Paque and MACS medthod, batch type method of direct ex vivo expansion through membranes and bioreactor of perfusion method of direct ex vivo expansion through membranes, and compared these results. Ex vivo expansion of HSCs compared to initial number of HSCs in UCB was found to be 6.6 folds when was used as a rinsing solution in batch type of direct ex vivo expansion of HSCs through the PU-COOH membranes. Ex vivo expansion of HSCs in 3D culture was found to be worse than that in 2D culture using HSCs purified by Ficoll-Paque and MACS method. This is because pluripotent HSCs adhered on PU membranes tightly, and HSCs in PU membranes of 3D culture could not be detached even after pipetting with washing solution. However, ex vivo expansion of HSCs in bioreactor of perfusion method was found to be similar ex vivo expansion fold compared to that in 2D culture using HSCs purified by conventional Ficoll-Paque and MACS method, which needs longer working time (e.g., 5 hours). The bioassay of HSCs by colony-forming units where HSCs were cultured after purification of HSCs from UCB by conventional Ficoll-Paque and MACS method and by our direct ex vivo expansion method was also investigated. Perfusion type of direct ex vivo expansion method of HSCs from UCB through membranes can provide high ex vivo expansion fold with simple procedure and short working time for HSC purification before HSC culture.
|DC.subject||ex vivo expansion||en_US|
|DC.subject||hematopoietic stem cells||en_US|
|DC.subject||stem cell purification||en_US|
|DC.title||Separation and ex vivo expansion of hematopoietic stem cells from human blood by membrane filtration method and magnetic associated sorting method||en_US|
|DC.publisher||National Central University||en_US|