| dc.description.abstract | The method of detection E. coli of environmental takes a long time to estimate the results, if water is a life of leisure or drinking, the human body will be exposed to a high risk of illness, and thus cause to detection time. Although there are different rapid detection method have issued, but all have high costs, complicated operation and other shortcomings, therefore, this study will use absorption spectrometry, using the E. coli enzyme reaction substrate ONPG reaction, the reaction product by o - nitrophenol with absorption spectrophotometer measured by the effect of establishing the method of E. coli in order to improve the current shortcomings of time-consuming assays, and the study established measurement methods, requiring only added to the substrate 0.5mM can get in 35 minutes the acceptable linear correlation measurement mode, by actual measurement life of leisure water, within 35 minutes, measured the number of E. coli and the environment in the enzyme assay of membrane filter percentage difference between the lowest to the highest was 20.01% ~ 53.40%, the measuring quantity, the minimum to the maximum number of measurements ranging from 49,300,000 (CFU/100ml) ~ 247,800,000 (CFU/100ml), but in the waste, sewage, E.coli may have the same enzymes as biological interference and overestimate. | en_US |