| dc.description.abstract | Carbon nanotubes with their unique pseudo-one-dimensional nanostructures and related electronic, optical, and mechanical properties have been attracting much attention for potential biological applications, such as biosensors, bio-delivery, bio imaging and so on. Recently, carbon nanotubes have been explored as multipurpose innovative carriers for drug delivery and diagnostic applications. In this thesis, recent studies and advances toward bioapplications of carbon nanotubes are reviewed, followed by detailed reports on my research project concerning the use of stable complexes between carbon nanotubes and DNA for gene therapeutic capacity are mentioned.
In our project, we used 3’’-O-[(tert-butyl)dimethylsilyl]-2’’-deoxyadenosine (14) and 9-azido-4,7-dioxanonanoic acid (7) as our starting materials. The reaction with the adenosine derivative gave 5’’-O-(9-azido-4,7-dioxanonanyl)-2’’-deoxyadenosine (16). After that we successfully functionalized the carbon nanotubes by using the p-amino phenyl propargyl ether (19). Finally the functionalized carbon nanotube derivative (1) coupled with 5’’-O-(9-azido-4,7-dioxanonanyl)-2’’-deoxyadenosine (16) by using click reaction results multi-adenosine derivatives on to carbon nanotubes (2).
Then we used infrared spectroscopy, Raman spectra, thermogravimetric analysis spectra and HRSTEM spectra to confirm wrapping of multi-adenosine derivatives on to carbon nanotubes. Importantly, when we compared to previous data that the click reaction yield is enhanced to 77%. It indicates that the click reaction is simple and good selectivity with high efficiency. The functionalised carbon nanotubes has high specificity in drug delivery and targetting DNA sequence. The functionalised carbon nanotubes has the advantage to cure the diseases in efficient way with less side effects.
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