| dc.description.abstract | In recent years, the research of regenerative medicine focus on the studying and utilizing stem cells. Current sources of stem cells include embryonic stem cells (ESCs) and adult stem cells (ASCs). However, there are numerous ethical concerns exist in ESCs and as to ASCs, which usually obtained from the bone marrow through an invasive procedure. The placenta-derived multipotent cells (PDMCs) are an ethically uncontroversial source, and the cells exhibit the ability to differentiate into many cellular types, including osteoblasts, adipocytes, hepatocytes, and neurons. Therefore, PDMCs could be the considerable alternative resources of mesenchymal stem cell.
It has been found that under 3-isobutyl-1-methylxanthine (IBMX) inductions, the PDMCs were capable of differentiating into neural-like cells; however, the signal transduction scenario and molecular mechanism behind the phenomenon are still unknown, therefore, we attempts to study this process by phosphoproteomic approaches. After phosphoprotein enrichment with IMAC from IBMX induced PDMCs, two dimensional gel electrophoresis following LC/MS-MS identification were enrolled and the phosphorylated proteins specific induced by IBMX were identified and analyzed by the signal transduction database tool.
After Western blot validation and literature searches, we found that IBMX would induce phosphorylation of protein kinase C (PKC), extracellular signal-regulated kinase (ERK), and c-Jun and their sequential phosphorylation might involve in the signal transduction in the neural cell differentiation. Besides, cell morphology changes would also be induced through dephosphorylation of other proteins such as Heat Shock Protein 27 (HSP27). However, the phosphorylation regulations and their physiological roles need to be further validated. The information provide by this thesis may help us decipher the mechanism of neuron differentiation and may contribute to the neural degenerative disease treatment in the future.
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