| dc.description.abstract | To custom-tailor biomaterial surfaces for tissue engineering
application, we developed chitosan/alginate composite surfaces using
electrospinning technique. Firstly, chitosan and alginte were solely
electrospun, respectively. Poly (ethylene oxide) (PEO) was added to both
chitosan and alginte solutions, and the effect of polymer composition on
fibrous morphology was studied through scanning electrical microscopy
(SEM). In addition, viscosities of polymer solution were also analyzed,
demonstrating that higher concentrations of polymer caused higher
viscosities, which directly determined the morphology of nanofibrous
structure. Then, the relationship between flow rates and fiber deposition
was also studied. By controlling the flow rates of chitosan/PEO and
alginate/PEO solution separately, these nanofibers were able to be spun
simultaneously with different deposition ratios. Finally, the composite
nanofibrous surfaces were applied for cell culture. Because cell
morphologies were different in chitosan and alginate nanofibrous surfaces,
we may regulate cell morphologies by controlling the ratios of nanofibers,
About biocompatibility, it was found that nanofibers can improve the cell
viability over chitosan and alginate films. In addition, composite
nanofibers even demonstrated greater biocompatibility than that of
chitosan and alginate fibers. These results suggested that the ratio of
nanofiber can be finely controlled through simultaneous electrospinning
technique, and we may manipulate composite surface properties, which
should be beneficial to biomaterial application.
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