博碩士論文 88224008 詳細資訊

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姓名 林奕成( Yi-Chen Lin)  查詢紙本館藏   畢業系所 生命科學系
論文名稱 陰離子界面活性劑sodium dodecylbenzene sulfonate分解菌篩選與脫磺酸酵素研究
(Isolation of a bacterium degrading sodium dodecylbenzene sulfonate and characterization of desulfonation enzyme)
★ 鄰苯二酚加氧酵素的熱穩定性提昇研究★ Triton X-100 分解菌之分離和分解酵素之特性研究
★ Triton X-100加氧酵素之純化與定性★ Lactobacillus reuteri於酸性與膽鹽環境中之蛋白質體研究
★ 蕃茄根部受銅逆境之基因調控★ Pseudomonas nitroreducens TX1 異化辛基苯酚聚氧乙基醇之功能性蛋白質體學:以二維電泳法分析等電點4-8之蛋白質表現
★ Pseudomonas nitroreducens TX1之具耗氧活性之麩胺酸合成酶之單離★ 人類細胞株生產含多種亞型的 干擾素-a之蛋白質體學研究
★ 辛基苯酚之分解:分解菌和生物復育之菌相研究★ 分解辛基苯酚聚氧乙基醇之耗氧酵素(二氫硫辛醯胺脫氫酶)的純化與定性
★ AtNPR1轉殖番茄之性狀分析及抗病機制研究★ Pseudomonas putida TX2分解辛基苯酚聚氧乙基醇及其具雌激素活性代謝物之研究
★ 以功能性蛋白質體學研究Pseudomonas nitroreducens TX1生長於辛基苯酚聚氧乙基醇之代謝與逆境反應★ 以功能性蛋白質體學研究Pseudomonas putida TX2生長於 辛基苯酚聚氧乙基醇與辛基苯酚之代謝與逆境反應
★ 以功能性基因體學研究細菌異化辛基苯酚 聚氧乙基醇及抗逆境之基因★ Pseudomonas nitroreducens TX1中二氫硫辛醯胺脫氫酶分解辛基苯酚聚氧乙基醇之研究
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摘要(英) Linear alkylbenzene sulfonates (LAS) are the most widely used anionic surfactants today. These compounds accumulate in the environment and show toxic effects on organisms in polluted water. This strain utilized sodium dodecylbenzene sulfonate (DBS) as sole source of sulfur in a minimal salts medium containing 0.3 % succinate. The bacterium can’t be identified by BioLog method in Gram-negative database. Further identification by 16S rDNA sequence was performed and showed 98 % identity to many Enterobacter species. Phylogenetic tree was constructed from an alignment of 1,193 nucleotides of 16S rDNA sequences showing the highest phylogenic relationship with Enterobacter agglomerans. And the bacterium was designated Enterobacter sp. SH3. The growth of strain was demonstrated as a function in the decreasing of DBS, which was analyzed by liquid chromatography-mass spectroscopy (LC-MS). It’s specific that Enterobacter. sp. SH3 can utilize several alkanesulfonates and aromatic sulfonates as sole sulfur source to grow. The action of the release of sulfite from DBS was measured using Ellman’s reagent followed by the absorbance increased at 430 nm as an enzyme assay. The highest enzyme activity was observed in the addition of 500 mM NADPH and 3 mM FMN in 0.3 ml 10 mM Tris-HCl, pH 9.0. The preliminary biochemical feature of the enzyme acting in Enterobacter sp. SH3 was showed to be similar to an FMNH2-dependent alkanesulfonate monooxygenase from Escherichia coli EC1250. A series of column chromatography was applied to purify the desulfonation enzyme. The active fraction from chromatofocusing chromatography was at pI of 5.18-4.12. The native molecular weight was at the range of 60-150 kDa by Sephacryl S-200 gel filtration chromatography. This highly purified desulfonation enzyme pool (240 mg) was further separated by 2D-gel electrophoresis and identified by MALDI-TOF
關鍵字(中) ★ 界面活性劑
★ 生物分解
★ 菌種鑑定
★ 脫磺酸
關鍵字(英) ★ surfactant
★ biodegradation
★ bacterium identifica
論文目次 目錄------------------------------------------------------------------------------I
四、Enterobacter sp. SH3對TS的降解-------------------------------34
二、Enterobacter sp. SH3的生長特性探討---------------------------39
三、Enterobacter sp. SH3對DBS、TS的生物轉化-----------------40
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指導教授 黃雪莉(Shir-Ly Huang) 審核日期 2001-7-20
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