博碩士論文 88321016 詳細資訊




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姓名 梁祥發( Xiang-Fa Liang)  查詢紙本館藏   畢業系所 化學工程與材料工程學系
論文名稱 以Lactobacillus reuteri菌發酵glycerol生成reuterin做為生物組織材料天然滅菌劑的探討
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摘要(中) 生物組織材料在臨床上,可以用來暫時性或永久性的取代病人缺損的組織或器官。這些生物組織材料在經過適當的交聯劑交聯處理後,可以減低這些異種生物組織材料來源的抗原性,並增加生物組織材料在植入人體後抵抗酵素分解的能力。然而這些經過交聯劑化學修飾的生物組織材料,在植入人體之前仍需經過適當的滅菌處理,以大量減少或完全去除材料上所帶有的微生物,避免病人遭受致病微生物的感染。然而目前臨床上對於生物組織材料,尚未有一個理想、適合的滅菌方法。
本實驗室目前正著手研究一種新的天然滅菌劑-Reuterin。Reuterin是由人或動物的腸胃道所分離出的乳酸菌Lactobacillus reuteri在厭氧的狀態下發酵glycerol後所產生的滅菌物質。其抑菌作用機制可能與RNA氧化還原
關鍵字(中) ★ 滅菌劑
★ reuterin
★ Lactobacillus reuteri
★ 生物組織材料
關鍵字(英) ★ reuterin
★ Lactobacillus reuteri
★ biological tiss
論文目次 摘要Ⅰ
目錄Ⅲ
圖索引Ⅵ
第一章 緒論1
1.1 生物組織材料1
1.2 滅菌方法1
1.3天然滅菌劑reuterin2
1.3.1 Lactobacillus reuteri3
1.3.2 Reuterin3
1.4 研究動機與目的4
第二章 Lactobacillus reuteri發酵glycerol生成reuterin6
2.1 研究目的6
2.2 實驗材料6
2.2.1 Lactobacillus strain6
2.2.2 Reuterin的製備6
2.3 實驗方法8
2.3.1 Reuterin分離純化8
2.3.2 FT-IR、GC-MS及NMR分析9
2.3.3 L. reuteri選擇及鑑定10
2.3.4 L. reuteri發酵glycerol最適化條件的探討10
2.3..4.1 生長曲線10
2.3.4.2 發酵glycerol最適化條件探討12
2.4 實驗結果與討論13
2.4.1 Reuterin分離純化13
2.4.2 FT-IR、GC-MS及NMR分析14
2.4.3 乳酸菌選擇及鑑定18
2.4.4 L. reuteri發酵glycerol最適化條件的探討19
2.4.4.1 生長曲線19
2.4.4.2 發酵glycerol最適化條件探討21
2.5 結論25
第三章 Reuterin滅菌性質與細胞毒性測試26
3.1 研究目的26
3.2 實驗材料26
3.2.1 Reuterin製備26
3.2.2 Genipin交聯組織27
3.2.3 實驗菌株與細胞28
3.3 實驗方法29
3.3.1 Reuterin滅菌性質29
3.3.2 細胞毒性測試31
3.3.2.1 SEM觀察細胞生成情形31
3.3.2.2組織病理切片32
3.3.2.3 MTT assay34
3.4 實驗結果與討論35
3.4.1 Reuterin的滅菌性質35
3.4.2 細胞毒性測試39
3.4.2.1 SEM觀察細胞生成情形39
3.4.2.2組織病理切片40
3.4.2.3 MTT assay44
3.5 結論45
第四章Reuterin穩定性探討46
4.1 研究目的46
4.2材料與方法46
4.2.1 Reuterin製備46
4.2.2 實驗方法46
4.2.2.1 Reuterin溶液穩定性測試46
4.2.2.2 Reuterin降解後物質的滅菌性質以及細胞相容性47
4.3 實驗結果與討論51
4.3.1 Reuterin溶液穩定性測試51
4.3.2 Reuterin降解後的滅菌性質與細胞毒性測試54
4.4 結論58
參考文獻59
附件63
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指導教授 高振宏(C. Robert Kao) 審核日期 2001-7-13
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