摘要(英) |
Over past decade, Photodynamic therapy(PDT) is one of most potential way in cancer research. In classic PDT, photosensitizer(PS) is injected into tumor region, then expose to specic wavelength of light to excite PS to induce singlet oxygen dealing damage directly on tumor cell. Recent study shows that treating PDT on surgical resection tumor or culture tumor cell ex-vivo make cancer vaccine which stimulate immune system to cure and prevent caner.
The mechanism of PDT-treated vaccine is not clear, but research shows that the level of heat shock protein 70(hsp70) expression in cancer cell after PDT treated is correlate with their immunogenicity. In addition, after PDT treated, cancer cell release exosome, a kind of membrane-bound nanovesicle, induce macrophage to release TNF-α as an indicator of macrophage activation.
In this research, we choose mouse lewis lung carcinoma and chlorin e6 to develop cancer vaccine. We change concentration of Ce6 and recovering time after PDT to optimize vaccine by detecting cell surface hsp70, and induce macrophage release TNF-α by exosome to test immunogenicity of vaccine. |
參考文獻 |
[1] Mladen Korbelik. Cancer vaccines generated by photodynamic therapy. Photochem. Photobiol. Sci., 10(5):664{669, 2011.
[2] Sandra O Gollnick, Lurine Vaughan, and Barbara W Henderson. Generation of eective antitumor vaccines using photodynamic therapy. Cancer research, 62(6):1604{1608, 2002.
[3] Mladen Korbelik and Jinghai Sun. Photodynamic therapy-generated vaccine for cancer therapy. Cancer Immunology, Immunotherapy, 55(8):900{909, 2006.
[4] Mladen Korbelik, Jinghai Sun, and Ivana Cecic. Photodynamic therapy{induced cell surface expression and release of heat shock proteins: relevance for tumor response. Cancer research,65(3):1018{1026, 2005.
[5] Xiaohong Chen, Qun Tao, Hai Yu, Lihuang Zhang, and Xuetao Cao. Tumor cell membrane-bound heat shock protein 70 elicits antitumor immunity. Immunology letters, 84(2):81{87, 2002.
[6] Virginia L Vega, Monica Rodrguez-Silva, Tiany Frey, Mathias Gehrmann, Juan Carlos Diaz, Claudia Steinem, Gabriele Multho, Nelson Arispe, and Antonio De Maio. Hsp70 translocates into the plasma membrane after stress and is released into the extracellular environment in a membrane-associated form that activates macrophages. The Journal of Immunology, 180(6):4299{4307,2008.
[7] Huang-Ge Zhang and William E Grizzle. Exosomes: a novel pathway of local and distant intercellular communication that facilitates the growth and metastasis of neoplastic lesions. The American journal of pathology, 184(1):28{41, 2014.
[8] M Korbelik, B Stott, and J Sun. Photodynamic therapy-generated vaccines: relevance of tumour cell death expression. British journal of cancer, 97(10):1381{1387, 2007.
[9] Abhishek D Garg, Dmitri V Krysko, Peter Vandenabeele, and Patrizia Agostinis. Damps and pdt-mediated photo-oxidative stress:exploring the unknown. Photochemical & Photobiological Sciences,10(5):670{680, 2011. |