利用鹼和Aspergillus niger處理稻稈以提升甲烷生產於厭氧共發酵系統

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施智雄(Jhih-Syong Shih) 查詢紙本館藏

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化學工程與材料工程學系

論文名稱

利用鹼和Aspergillus niger處理稻稈以提升甲烷生產於厭氧共發酵系統

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摘要(中)

利用農業廢棄物進行甲烷厭氧發酵逐漸受到全球的重視並且正積極研究中，由於它具有許多經濟與環境上的優點，然而木質纖維素難以被生物所降解，須要透過前處理的方式改善它的生物降解性。
在本實驗中，利用鹼(NaOH)預處理及Aspergillus niger生物預處理的方式處理稻稈，探討經過稻稈經過不同預處理方式後，對稻稈的組成的變化和甲烷產氣的影響。
由結果顯示，利用鹼和Aspergillus niger生物預處理稻稈方式處理稻稈被證明是個有效的方法且改善了稻稈的生物可降解性和提升了甲烷的產氣量和縮短了產氣時間。其中鹼處理稻稈再經過5天的生物預處理有最高的甲烷產氣量為1834.20 mL和最高的還原醣累積量為2.229 g/l，與未處理稻稈相比，甲烷氣的產量增加了3.17倍。稻稈經鹼處理後移除了45.5%的木質素含量，之後經過5天和10天的生物預處理後，鹼處理後的稻稈纖維素組成從一開始的81.13% 減少至72.86%和60.97%，這結果說明了經過鹼預處理與生物預處理後，有效改善了稻稈的生物可降解性與提升了甲烷的產量。

摘要(英)

Methane production from a variety of agricultural wastes through
anaerobic digestion technology is growing worldwide and is actively studying because of its economic and environmental benefits. However, lignocelluloses is difficultly to use by microorganism, so we have to improve its biodegradability through the pretreatment.
In this research, we investigated the effects of alkali (NaOH) and the biological treatment of rice straw using Aspergillus niger in terms of the changes in the components of the treated rice straw and the effect of the methane production .
According to this study, the alkali (NaOH) and biological pretreatment with Aspergillus niger proved to be an efficient method to improve biodegradability and to enhance the biogas production of rice straw and the methane production time were shortened. Alkali treatment and then after five days biological pretreatment of rice straw has the highest methane production, it is 1834.20 mL and the highest cumulative amount of reducing sugars is 2.229 g / l. Compared to untreated controls the pretreated rice straw yielded by 3.17 times more methane production. The alkali (NaOH) pretreatment of rice straw was removed 45.5% of the lignin content, and then after 5 and 10 days biological pretreatment of rice straw, the cellulose contents was reduced from 81.13% to 72.86% and 60.97%, respectively. This result shows that the biodegradability of rice straw and enhanced methane production were improved through alkaline pretreatment and biological pretreatment.

關鍵字(中)

★ 稻桿
★ 前處理
★ 甲烷生產

關鍵字(英)

★ rice straw
★ pretreatment
★ Methane production

論文目次

摘要 i
英文摘要 ii
致謝 iii
目錄 iv
圖目錄 viii
表目錄 xi
第一章緒論 1
1-1 研究動機 1
1-2 研究目的 2
第二章文獻回顧 4
2-1 生質能源 4
2-2 生質甲烷 4
2-2.1 生質甲烷發酵過程 4
2-2.2 甲烷厭氧發酵的影響因素 7
2-3 稻稈 10
2-3.1 稻稈各地區產量分布與組成 10
2-3.2 稻桿的前處理方式 14
2-4 纖維酵素 20
2-4.1 纖維酵素水解 20
2-4.2 纖維素酶之來源 22
2-4.3 纖維素水解酵素之誘導 25
2-5 木質纖維素應用於甲烷生產 26
2-6 國內豬糞的利用與豬隻頭數 27
第三章材料與方法 29
3-1 實驗流程設計 29
3-2 實驗材料 30
3-2.1 稻稈 30
3-2.2 豬糞 30
3-2.3實驗菌株 31
3-2.4 實驗藥品 32
3-2.5 實驗儀器與設備 33
3-3 實驗方法 35
3-3.1菌種保存 35
3-3.2培養基組成 35
3-3.3 稻稈的鹼處理 39
3-3.4 甲烷厭氧發酵 39
3-4 分析方法 41
3-4.1 總固體量 ( Total Solid，TS) 測定 41
3-4.2 揮發性固體量 ( Volatile Solids，VS ) 測定 41
3-4.3 日產氣量測定 42
3-4.4 氣體層析儀---火焰點火偵測器操作程序(GC—FID) 42
3-4.5 甲烷含量分析方法 45
3-4.6 揮發性有機酸分析方法 47
3-4.7 還原醣定量分析方法 48
3-4.8 酵素活性之測定 49
3-4.9 稻桿組成分析 51
第四章實驗結果與討論 55
4-1 甲烷厭氧發酵 55
4-1.1不同預處理方式稻稈之甲烷日產氣量與甲烷百分比 55
4-1.2 不同預處理方式稻稈之生物氣與甲烷累積產氣量 58
4-2 未處理或鹼處理稻稈經生物預處理後對稻稈的影響 61
4-2.1未處理或鹼處理稻稈經生物預處理的還原醣累積情形 62
4-2.2不同預處理方式對稻稈的木質素、半纖維素和纖維素的組成的影響 64
4-2.3未處理或鹼處理稻稈經生物預處理的酵素活性 69
4-2.4 未處理或鹼處理稻稈經生物預處理時pH值和揮發性脂肪酸累積 72
第五章結論與建議 78
5-1 結論 78
5-2 建議 80
第六章參考文獻 81

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指導教授

徐敬衡(Chin-Hang Shu)

審核日期

2013-7-29

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