北台灣腐生絲孢菌綱之採集與描述

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劉力嘉(Li-chia Liu) 查詢紙本館藏

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生命科學系

論文名稱

北台灣腐生絲孢菌綱之採集與描述
(Characterization of selected saprobic hyphomycetes from Northern Taiwan)

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摘要(中)

絲孢菌綱 (Hyphomycetes) 菌類隸屬於半知菌亞門 (Deuteromycotina), 是一群不具有有性世代或者有性世代尚未被發現的類群。在台灣，尚有許多真菌未被發現、進行鑑定和描述繪圖。這項研究的重點是針對腐生絲孢菌綱的鑑定和描述繪圖。自 2012 至 2014 年從北台灣共 10 處不同自然生態環境且海拔由 0 至 1800 公尺之區域中採集各類枯枝腐葉共 10 種宿主植物超過 16 個樣品。 Clonostachys epichloe, Cl. rogersoniana, Cylindrocladium floridanum, Selenodriella fertilis, Tubercularia longispora 和 Verticillium insectorum 等六種台灣新紀錄。此外尚發現 Myrothecium sp. 1, Myrothecium sp. 2, 及 Venustosynnema sp. 等三種世界新種。其中Venustosynnema sp. 同時擁有周邊剛毛及中央剛毛， Myrothecium sp. 1 其 synnemata 周邊包覆豐富的勾狀邊緣菌絲， Myrothecium sp. 2 有少量的鉤狀邊緣菌絲和大量剛毛圍繞在 sporodochia 周邊。研究中菌類的型態特徵描寫以生長在玉米粉瓊脂與天然基質培養的形貌進行比較，並描述和說明， Cl. rogersoniana 和 T. longispora 則是第一次在天然基質中被描述。在對比培養形態學中， Cl. rogersoniana 在自然基質上很少形成 Verticillium-like 的分生包子梗。研究中使用核內 rDNA 的 ITS 區域 DNA 序列數據進一步證實形態學物種概念。

摘要(英)

Hyphomycetes belonging to Fungi Imperfecti subphylum (Deuteromycotina) is a group of fungi that do not have sexual generation or sexual generation yet to be discovered. In Taiwan, many fungi have not yet been found, identified and characterized. This study is focused on the identification and characterization of saprobic hyphomycetes. More than 16 samples on 10 host plants were collected in this study in Northern Taiwan. Clonostachys epichloe, Cl. rogersoniana, Cylindrocladium floridanum, Selenodriella fertilis, Tubercularia longispora, and Verticillium insectorum are new records to Taiwan. In addition, Venustosynnema sp. nov., Myrothecium sp. 1, Myrothecium sp. 2 are new species of the world. Venustosynnema sp. has central setae and peripheral setae, Myrothecium sp. 1 has abundant hook-like marginal hairs surrounding entire synnemata, and Myrothecium sp. 2 has a small amount of hook-like marginal hairs and numerous setae surrounding sporodochia. The morphology of specimens derived from cultures grown on CMA and from natural substrata was compared and described and illustrated. Cl. rogersoniana and T. longispora are described from natural substrata for the first time. In contrast to culture morphology, Cl. rogersoniana on the natural substrate rarely forms Verticillium-like conidiophores. DNA sequence data of ITS regions within nuclear rDNA confirmed the morphological species concept.

關鍵字(中)

★ 絲孢菌
★ 形態學

關鍵字(英)

★ Hyphomycetes
★ Mophology

論文目次

目錄　　　　　　　

中文摘要 i
英文摘要 ii
誌謝 iii
目錄 iv
圖目錄 vi
表目錄 viii
Abbreviation Index ix
1 Introduction 1
2 Materials and methods 7
2.1 Specimen preservation 7
2.2 Light-microscopes 7
2.3 Isolation of fungi 8
2.4 Media 8
2.5 CMA ager with Banana Leaf 9
2.6 DNA isolation 9
2.7 PCR 11
2.8 Protocol for PCR 12
2.9 Agarose gel electrophoresis 14
2.10 Purification of PCR products 14
2.11 DNA sequencing 16
2.12 Morphological and molecular identification 16
3 Results 17
3-1 Result analysis 17
3-2 Description of species 18
3.2.1 Clonostachys epichloe 18
3.2.2 Clonostachys rogersoniana 21
3.2.3 Cylindrocladium floridanum 24
3.2.4 Cylindrocladium cf. ilicicola 27
3.2.5 Monocillium sp. 29
3.2.6 Myrothecium sp. 1 31
3.2.7 Myrothecium sp. 2 34
3.2.8 Selenodriella fertilis 38
3.2.9 Tubercularia longispora 40
3.2.10 Venustosynnema sp. nov. 43
3.2.11 Verticillium insectorum 46
4 Discussion 48
5 References 52
6 Appendixes 100

參考文獻

References

Buzina, W., Braun, H., Freudenschuss, K., Lackner, A., Habermann, W. and Stammberger, H. (2003). Fungal biodiversity--as found in nasal mucus. Medical Mycology. 41 (2):149-161.
Castaneda Ruiz, R. F., Gusmao, L. F. P., Oliveira de Moraes, J. V., Leao-Ferreira, S. M., Saikawa, M.; Minter, D. W. and Stadler, M. (2009). Two setose anamorphic fungi: Ampullicephala gen. nov. and Venustosynnema grandiae sp. nov. Mycotaxon 109: 275–288.
Castaneda Ruiz, R. F., T. Iturriaga, D. W., Minter, M., Saikawa, G. Vidal. & S. Velazquez-Noa. (2003). Microfungi from Venezuela. A new species of Brachydesmiella, a new combination, and new records. Mycotaxon. 85: 211-229.
Castaneda, R. F. and Kendrick, B. (1990). Conidial fungi from Cuba: I. University of Waterloo Biology Series. 32: 1-53.
Castaneda-Ruiz, R. F. (1986). Deuteromycotina de Cuba. Hyphomycetes IV: 10-11.
Castaneda-Ruiz, R. F., Gusmao, L. F. P., Guarro J., Stchigel A. M., Stadler M., Saikawa M. and Leao-Ferreira S. M. (2008). Two new anamorphic fungi from Brazil: Dictyochaetopsis polysetosa and Myrothecium compactum. Mycotaxon 103: 1-8.
Castlebury, L. A., Rossman, A. Y., Sung, G. H., Hyten, A. S. and Spatafora, J. W. (2004). Multigene phylogeny reveals new lineage for Stachybotrys chartarum, the indoor air fungus. Mycological Research. 108 (PT 8), 864-872.
Chen, J. L. (1994). Taxonomic Study of the Hyphomycetes, Deuteromycotina from Taiwan. Ph. D. thesis, National Taiwan Universerity.
Crous, P. W. (2002). Taxonomy and Pathology of Cylindrocladium (Calonectria) and Allied Genera. The American Phytopathological Society, St. Paul, Minnesota, U.S.A.
Crous, P. W., Groenewald, J. Z., Risede, J. M., Simoneau, P. and Nigel, L. H. J. (2004). Calonectria species and their Cylindrocladium anamorphs: species with sphareopedunculate vesicles. Studies in Mycology. 50: 415–430.
DiCosmo, F., Michaelides, J. and Kendrick, B. (1980). Myrothecium tongaense anam.-sp. nov. Mycotaxon 12: 219-224.
Domsch, K. H., Gams, W. and Anderson, T. H. (2007). Compendium of Soil Fungi. IHW-Verlag Eching.
Frohlich, J. and Hyde, K. D. (1999). Biodiversity and Conservation. 8: 977-1004.
Gams, W. (1971). Cephalosporium-artige Schimmelpilze (Hyphomycetes). 1-262. Gustav Fischer Verlag.
Gardes, M. and Bruns, T. D. (1993). ITS primers with enhanced specificity for Basidiomycetes – Application to the identification of mycorrhizae and rusts. Molecular Ecology. 2: 113-118.
Gottlieb, D., Bhattacharyya, P. K., Anderson H. W., and Carter, H. E. (1948). Some properties of an antibiotic obtained from a species of streptomyces. Journal of Bacteriology. 55: 409.
Guu, J. R. (2007). Nectria-like fungi collected from forests in Taiwan. Ph. D. thesis, National Taiwan Universerity.
Hawksworth, D. L. (1991). The fungal dimension of biodiversity: magnitude, significance and conservation. Mycological Research. 95:641-655.
Hawksworth, D. L. (2001). The magnitude of fungal diversity: the 1.5 million species estimate revisited. Mycological Research. 105: 1422–1432.
Hawksworth, D. L. (2004). Fungal diversity and its implications for genetic resource collections. Study in mycology. 50: 9–18.
Hirooka, Y., Rossman, A. Y., and Chaverri, P. (2011). A morphological and phylogenetic revision of the Nectria cinnabarina species complex. Studies in Mycology. 68: 35-56.
Kang, J. C. and Crous, P. W. (2001). Species concepts in the Cylindrocladium floridanum and Cy. spathiphylli complexes (Hypocreaceae) based on multi-allelic sequence data, sexual compatibility and morphology. Systematic and Applied Microbiology. 24 (2): 206-217.
Kurtzman, C. P. and Robnett, C. J. (1997). Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 5’ end of the large-subunit (26S) ribosomal DNA gene. Journal of Clinical Microbiology. 35: 1216-1223.
Lodge, D. J., and Cantrell, S. (1995). Fungal communities in wet tropical forests: variation in time and space. Canadian Journal of Botany. 73: 1391-1398.
Lombard, L., Crous, P. W., Wingfield, B. D. and Wingfield, M. J. (2010). Phylogeny and systematics of the genus Calonectria. Studies in Mycology. 66: 31–69.
Mangandi, J. A., Seijo, T. E. and Peres, N. A. (2007). First Report of Myrothecium roridum Causing Myrothecium Leaf Spot on Salvia spp. In the United States. Plant Disease. 91 (6): 772.
Matsushima, T. (2003). Matsushima Mycological Memoirs 10, published by the author, Kobe, Japan.
Moreira, G. M. (2012). Caracterizacao de species de Clonostachys e avaliacao do parasitismo a Botrytis cinerea. Master thesis, Universidade Federal de Lavras.
Nag Raj, T.R. (1993). Coelomycetous Anamorphs with Appendage Bearing Conidia. Mycologue Publications, Waterloo.
Petch, T. (1931). Notes on entomogenous fungi. Transactions of the British Mycological Society. 16(1): 55-75.
Pirozynski, K. A. and Hodges, C. S. (1973). New Hyphomycetes from South Carolina. Canadian Journal of Botany. 51: 157-173.
R. Kirschner. (2006). New records of Clonostachys epichloe, a mycoparasitic fungus on the grass-parasitic ascomycete Epichloe typhina for Europe. Feddes Repertorium. 117: 307-311.
Rao, V. and Hoog, G.S. de. (1983). A new species of Myrothecium. Persoonia. 12: 99-101.
Rungjindamai, N., Sakayaroj, J., Somrithipol, S., Plaingam, N. and Jones, G. (2012). Phylogeny of the Appendaged Coelomycete Genera: Pseudorobillarda, Robillarda, and Xepiculopsis Based on Nuclear Ribosomal DNA Sequences. Cryptogamie, Mycologie. 33(3):319-332.
Saksena, S. B. (1955). A new fungus Monocillium indicum gen. et sp. nov. from soil. Indian Phytopathology. 8:9-12
Samuels, G. J. (1988). Fungicolous species of Hypocreopsis, Nectriopsis, Nectria, Peristomialis, and Trichonectria. Memoirs of the New York Botanical Garden. 48: 1-78.
Schroers, H. J. (2001). A monograph of Bionectria (Ascomycota, Hyprcreales, Biobectriaceae) and its Clonostachys anamorphs. Studies in Mycology. 46: 104-153.
Seifert, K. A. (1985). A monograph of Stilbella and some allied Hyphomycetes. Studies in Mycology. 27:1-235.
Seifert, K. A., Louis-Seize, G. and Sampson, G. (2003). Myrothecium acadiense, a new hyphomycete isolated from the weed Tussilago farfara. Mycotaxon. 87: 317-327.
Seifert, K., Morgan-Jones, G., Gams, W. and Kendrick, B. (2011). The Genera of hyphomycetes. CBS Biodiversity Series. 9: 1-997. CBS-KNAW Fungal Biodiversity Centre, Utrecht, Netherlands.
Summerbell, R. C., Gueidan, C., Schroers, H-J., de Hoog, G. S., Starink, M., Arocha Rosete, Y., Guarro, J. and Scott, J. A. (2011). Acremonium phylogenetic overview and revision of Gliomastix, Sarocladium,and Trichothecium. Study in mycology. 68: 139-162.
Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution. 30(12): 2725-2729.
Tode, H. J. (1790). Nova Fungorum Genera Complectens. i-viii. Fungi Mecklenburgenses Selecti. Fasc. 1: 1-50.
Tulloch, M. (1972). The genus Myrothecium Tode ex Fr. Mycological Papers. 130: 1-42.
Varghese, K. I. M. and Rao, V. G. (1978). Two new setose hyphomycetes from India. Botaniska notiser. 131:215-217.
Wang, W. Y. (1992). Quart. Journ. Chinese Forestry. 7( 2 ) : 184.
Weis, M., Yang, Z. L. and Oberwinkler, F. (1998). Molecular phylogenetic studies in the genus Amanita. Canadian Journal of Botany. 76: 1170–1179.
White, T. J., Bruns, T., Lee, S. and Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: PCR Protocols: a guide to methods and applications. (Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds). Academic Press, New York, USA: 315–322.
Wu, B., Tian, J., Bai, C., Xiang, M., Sun, J. and Liu, X. (2013). The biogeography of fungal communities in wetland sediments along the Changjiang River and other sites in China. The ISME Journal. 7 (7): 1299-1309.
Wu, L., Han, T., Li, W., Jia, M., Xue, L., Rahman, K. and Qin, L. (2013). Geographic and Tissue Influences on Endophytic Fungal Communities of Taxus chinensis var. mairei in China. Current Microbiology. 66 (1): 40-48.

指導教授

羅南德(Roland Kirschner)

審核日期

2014-6-23

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