結合表面電漿共振及溫度控制於免疫球蛋白鍵結之檢測分析

以作者查詢圖書館館藏

、以作者查詢臺灣博碩士

、以作者查詢全國書目

、勘誤回報

、線上人數：9

、訪客IP：3.144.248.24

姓名

張綺珊(Chi-Shan Chang) 查詢紙本館藏

畢業系所

光電科學與工程學系

論文名稱

結合表面電漿共振及溫度控制於免疫球蛋白鍵結之檢測分析
(Immunoglobulin Binding Analysis by Utilizing a Surface Plasmon Resonance Phase-Measurement System with Temperature Control)

相關論文

★ 以GATE模型及系統矩陣演算法重建SPECT螺旋影像	★ LED檯燈視覺舒適度研究
★ 表面電漿共振系統之相位擷取與分析	★ 人眼眼球模型與視覺表現之模擬分析研究
★ 白光LED之視覺生理效應評估	★ 不同色溫螢光燈用於辦公室照明之視覺效應研究
★ 表面電漿共振儀之動態相位偵測技術與微量生物分子檢測應用	★ 二次通過成像架構量測人眼的光學系統品質
★ 週期性奈米金屬結構對拉曼散射訊號增強之研究	★ 日眩光要因分析研究
★ 非球面檢測之迭代相移干涉與子孔徑相位接合演算法開發	★ 應用可容忍隨機位移之相移干涉術於相位式表面電漿共振系統之穩定度增進
★ 以偵測任務及系統效能評估找尋多針孔微單光子放射電腦斷層掃描系統之最佳化配置	★ 以二次通過成像量測架構及降低誤差迭代演算法重建人眼之點擴散函數
★ 多陽極光電倍增管閃爍相機之訊號讀出系統與高效最大可能性位置估算演算法開發	★ 小動物針孔單光子放射電腦斷層掃描系統之系統校正與配置最佳化

檔案

[Endnote RIS 格式]

[Bibtex 格式]

[相關文章]

[文章引用]

[完整記錄]

[館藏目錄]

[檢視]

[下載]

本電子論文使用權限為同意立即開放。
已達開放權限電子全文僅授權使用者為學術研究之目的，進行個人非營利性質之檢索、閱讀、列印。
請遵守中華民國著作權法之相關規定，切勿任意重製、散佈、改作、轉貼、播送，以免觸法。

摘要(中)

大多生物分子在各種溫度下會有不同的活性反應，故本研究以干涉式相位量測的表面電漿感測器為基礎，加入溫度控制裝置，進行P極化光、不同濃度的鹽水實驗及免疫球蛋白IgG與Anti-IgG之鍵結實驗，並用五步演算法進行解相分析。以純水及鹽水實驗量測系統表現，得到的系統穩定度為0.4667度，系統靈敏度為1.617x10^3 (Degree/RIU)，系統折射率解析度可達2.886x10^-4 (RIU)。

本研究將IgG附著於金膜上，通入Anti-IgG進行鍵結，並討論在常溫下及有使用溫度控制，亦即生物體溫度(36 )下的實驗情況，在有使用溫度控制下的IgG及Anti-IgG之結合所產生的相位變化為6.72度，未使用溫度控制的相位變化為3.04度，兩者差異為3.68度。使用溫度控制裝置將溫度固定於實際生物分子的生存溫度時，將能從實驗過程中得到更接近於現實的生物分子活性反應，因此結合溫度控制裝置於表面電漿共振系統，將適用於進行更多種類的生物分子檢測。

摘要(英)

Most biomolecules have different reaction activities at various temperatures. In this study, a temperature control apparatus was incorporated into a surface plasmon resonance (SPR) sensor, which is based on the interferometric phase measurements. Three kinds of experiments were performed, including P-polarized light measurements, salt-water mixture measurements and antibody-antigen binding experiments. The acquired temporal intensity signals were processed by the Schwider-Hariharan five-step phase-shifting algorithm to obtain the phases. The achieved phase-detection stability was 0.4667 degrees, and the system sensitivity was 1.617x10^3 degrees/RIU (refractive index unit). In addition, the corresponding system sensitivity was 2.886x10^-4 RIU.

In the third experiment, IgG was first binded on the Au film, and then anti-IgG was pumped into the flow cell to bind with IgG. The experiments were performed at two temperatures: at room temperature and at 36 degrees Celsius. The phase changes during the binding process were 6.72 degrees at 36 degrees Celsius, and 3.04 degrees at room temperature. The difference in phase changes between the two temperatures was 3.68 degrees. By using temperature control, the experiment results would be closer to the reality, which enables more varieties of biomolecular reactions to be detected by this SPR system.

關鍵字(中)

★ 表面電漿共振
★ 溫度控制

關鍵字(英)

論文目次

摘要 I

ABSTRACT II

誌謝 IV

目錄 V

圖目錄 VIII

表目錄 XII

第一章緒論 1

1.1前言 1

1.2文獻回顧 1

1.2.1生物分子感測器-表面電漿感測器 1

1.2.2 SPR免疫檢測技術 4

1.2.3 免疫球蛋白結構 6

1.3 研究動機與目的 7

1.4 論文架構 7

第二章基本理論 8

2.1 表面電漿原理 8

2.1.1 表面電漿波之色散關係式 8

2.1.2 SPR電漿波的產生條件 12

2.2 KRETSCHMANN組態下系統反射係數 13

2.2.1 一層介面結構之系統反射係數 13

2.2.2 二層介面結構之系統反射係數 16

2.2.3 三層介面結構之系統反射係數 17

2.2.4 四層介面結構之系統反射係數 19

第三章相位式表面電漿共振儀 20

3.1 系統架構 20

3.2 系統解相方法 25

3.2.1 光學干涉原理 25

3.2.2 相移干涉術 27

3.3 感測片設計 30

3.4 儀控程式 32

第四章實驗方法 42

4.1 實驗藥品 42

4.2 藥品配製 43

4.3 生物分子固定化程序 43

4.4 實驗程序 44

第五章實驗結果與分析 46

5.1 P極化光的量測 46

5.2 系統穩定度與相位量測 48

5.3 鹽水實驗 49

5.4 生物分子IGG與ANTI-IGG結合量測 51

5.4.1 常溫下量測 51

5.4.2 生物體溫下( C)量測 54

第六章結論 58

參考文獻 59

參考文獻

[1]

L.C.Clark and C.Lyons, "Electrode system for continuous monitoring in cardiovascular surgery," Ann. NY. Acad. Sci., vol.102, pp. 29-45, 1962.

[2]

R.W. Wood, "On remarkable case uneven distribution of light in a diffraction grating spectrum," Phil. Magm., vol. 4, pp. 396-402, 1905.

[3]

R. H. Ritchie, "Plasmon losses by fast electrons in thin films.," Phys. Rev., vol. 106, pp. 874-887, 1957.

[4]

C. J. Powell and J. B. Swan,"Effect of oxidation on the characteristics loss spectra of aluminum and magnesium," Phys. Rev., vol. 118, pp. 640-643, 1960.

[5]

A. Otto, "Excitation of surface plasma waves in silver by the method of frustrated total reflection, "Z. Physik, vol. 216, pp. 398-410, 1968.

[6]

E. Kretschmann, "The determination of the optical constants of metals by excitation of surface plasmons," Z. Physik, vol. 241, p. 313, 1971.

[7]

C. Nylander, B. Liedberg and T. Lind, "Gas detection by means of surface plasmons resonance," Sensors and Actuator, vol. 3, pp. 79-88, 1982.

[8]

B. Liedberg, C. Nylander and I. Lundsr, "Surface plasmon resonance for gad detection and biosensing," Sensor and Actuators, vol. 4, pp. 299-304, 1983.

[9]

R. Karlsson, A. Michaelsson and L. Mattsson, "Kinetic-analysis of monoclonal antibody-antigen interactions with a new biosensor based analytical system," J Immunol Methods, 145(1-2), pp229-240, 1991.

[10]

Z. Salamon, H. A. Macleod and G. Tollin, "Surface plasmon resonance spectroscopy as a tool for investigating the biochemical and biophysical properties of membrane protein system.II:Applications to biological systems, " BBA-Bioenergetics, vol. 1331, pp. 131-152, 1997.

[11]

J. Homola and S. S. Yee, "Surface plasmon resonance sensors based on diffraction gratings and prism couplers: sensitivity comparison ," Sensors Actuator B-Chem, vol. 54, pp. 16-24, 1999.

[12]

R. P. H. Kooyman, H. Kolkman, J. V. Gent and J. Greve, "Surface plasmon resonance immunosensors : sensitivity considerations," Anal. Chim. Acta., vol. 213, pp. 35-45, 1988.

[13]

P. I. Nikitin, A. A. Beloglazov and V. E. Kochergin, "Surface plasmon resonance interferometry for biological and chemical sensing," Sensors Actuator B-Chem, vol. 54, pp. 43-50, 1999.

[14]

M. A. Cooper, "Optical biosensors in drug discovery, " Nat. Rev. Drug. Discov., vol. 1, pp. 515-528, 2002.

[15]

J. Ruzicka and E.H. Hansen, "Flow injection analysis. Part I. A new concept of fast continuous flow analysis. " Anal.Chim.Acta., vol. 78, p. 145, 1975.

[16]

J. Ruzicka and E. H. Hansen, "Flow injection analysis. Part II. Ultrafast determunation of phosphorous in plant material by continous flow spectrophotometry, " Anal. Chim. Acta., vol. 79, p. 79, 1975.

[17]

J. M. Woof and D. R. Burton, "Human antibody-Fc receptor interactions illuminated by crystal structures," Nat. Rev. Immunol., vol. 4, pp. 89-99, 2004.

[18]

C. A. Janeway, Jr, P. Travers, M. Walport, M. J. Shlomchik, "Immunobiology," New York: Garland Science, 2001, ISBN-10:0-8153-3642-X.

[19]

R. A. Rhoades, R. G. Pflanzer, "Human Physiology 4th," Thomson Learning, 2002, ISBN 0-534-42174-1.

[20]

C. Afaneh, M. J. Aull, S. Kapur, " Current Concepts in Kidney Transplantation," InTech., chapter 7, 2012, ISBN 978-953-51-0900-6.

[21]

邱國斌, 蔡定平, "金屬表面電漿簡介," 物理雙月刊, 廿八卷二期, pp. 472-485, 2006.

[22]

W. C. Tan, T. W. Preist, J. R. Sambles, and N. P. Wanstall, " Resonant tunneling of light through thin metal films via strongly localized surface plasmons," Phys. Rev., B 59, 12661, 1998.

[23]

J. A. Porto, F. J. Garcia-Vidal, and J. B. Pendry, "Transmission Resonances on Metallic Gratings with Very Narrow Slits," Phys. Rev. Lett., B 83, 2845, 1999.

[24]

林萱, "利用相位式表面電漿共振系統檢測免疫球蛋白鍵結之應用分析,"國立中央大學光電科學與工程學系照明與顯示科技碩士班碩士論文, 2012.

[25]

H. David, Halliday, Resnick, "Fundamentals of Physics, " Wiley, 2010, ISBN 978-0470556535.

[26]

顏嘉宏, "表面電漿共振系統之相位擷取與分析," 國立中央大學光電科學與工程學系研究所碩士論文, 2009.

[27]

P. Hariharan, B. F. Oreb and T. Eiju, "Digital phase-shifting interferometry: a simple error-compensating phase calculation algorithm," Applied Optics, vol. 26, pp. 2504-2506, 1987.

[28]

Available: http://refractiveindex.info/?group=LIQUIDS&material=Water.

指導教授

陳怡君

審核日期

2015-8-31

推文