組成成分與溫度對生物膜彈性性質的影響

以作者查詢圖書館館藏

、以作者查詢臺灣博碩士

、以作者查詢全國書目

、勘誤回報

、線上人數：16

、訪客IP：3.137.221.240

姓名

邱忠憲(Jhong-Sian Chiu) 查詢紙本館藏

畢業系所

生物物理研究所

論文名稱

組成成分與溫度對生物膜彈性性質的影響
(Composition and temperature effects on the elastic properties of membranes)

相關論文

★ 利用指向性圓二色光譜及多片層X光繞射技術研究抗菌胜肽與細胞膜作用的電荷效應	★ 使用指向性圓二色光譜和多片層X光繞射研究Magainin 2與混合脂質雙層膜的交互作用
★ 使用指向性圓二色光譜與多片層X光繞射技術進行daptomycin與細胞膜作用之結構研究	★ 細胞膜媒介麥角固醇與 α-crystallin 的作用
★ 羊毛脂固醇對 α-crystallin 和 SM 細胞膜間作用的影響

檔案

[Endnote RIS 格式]

[Bibtex 格式]

[相關文章]

[文章引用]

[完整記錄]

[館藏目錄]

[檢視]

[下載]

本電子論文使用權限為同意立即開放。
已達開放權限電子全文僅授權使用者為學術研究之目的，進行個人非營利性質之檢索、閱讀、列印。
請遵守中華民國著作權法之相關規定，切勿任意重製、散佈、改作、轉貼、播送，以免觸法。

摘要(中)

生物膜在細胞中扮演非常重要的角色，除了保護著細胞，許多由細胞膜媒
介的生物過程也維持細胞的活性，因此研究生物膜成為很重要的課題，近年來
很多學者把生物膜結構以及許多生物過程如胞吞現象、胞吐現象….等等，一一
地解謎，但仍有很多生物過程的原因及機制尚未明瞭，由於不少生物過程都有
透過生物膜的變形來完成，故我們用生物物理的方法來研究生物膜的彈性性
質，希望闡明其原因及機制。
不同細胞的生物膜有不同組成，這些組成和溫度皆對細胞的行為有重要的
影響，我們利用 X 光繞射在不同溫度中測量不同脂質混合物的結構，進一步藉
由改變滲透壓的方法得到其彈性性質，透過了解生物膜的彈性性質，可以更理
解各種重要的生物過程。
實驗結果發現不同的分子形狀會直接影響生物膜的自然曲率半徑，與
DOPE 形狀相同生物分子會使得自然曲率半徑不變，其餘與 DOPE 分子形狀不
同的生物分子會使得自然曲率半徑變大；帶電脂質使得自然曲率半徑也些微的
下降；最後蜂毒胜肽使得 DOPE 的六角型相態極為不易產生；在溫度上升時，
不同的脂質混合物的自然曲率半徑都有了些微的下降。

摘要(英)

Biological membranes play very important role for the cell. They not only protect
the cell but also mediate the biological processes to maintain the activity of cell.
Therefore, studying biological membranes is very important. In the past decades, a lot
of membrane structure and membrane-mediated biological processes have been studied.
However, most of the mechanisms of these biological processes are still the puzzles. In
this report, we studied the elastic properties of membranes by biophysical method for
further understanding these processes.
Different types of cells have varied membrane composition of lipids. The lipid
composition and temperature are able to affect the membrane-mediated biological
processes. We used x-ray diffraction to determine the structure of membranes in
different compositions as well as different temperatures. The elastic properties of
membranes were extracted from X-ray data by changing the osmotic pressure. We
should understand the biological processes more through the determination of the
elastic properties of membranes.
Consequently, we found different molecular shapes of the additives compare to
DOPE cause the increase of intrinsic radius of curvature. The negative charged lipids
decrease the intrinsic radius of curvature slightly. Melittin hold DOPE membranes in
the lamellar phase instead of hexagonal phase. Finally, the intrinsic radii of curvature
of all lipid mixtures decrease with temperature increasing.

關鍵字(中)

★ 生物膜
★ 抗菌生態
★ 脂質分子

關鍵字(英)

論文目次

摘要.................................................................................................................................i
Abstract..........................................................................................................................ii
致謝............................................................................................................................... iii
目錄............................................................................................................................... iv
圖目錄........................................................................................................................... vi
表目錄......................................................................................................................... viii
符號縮寫說明............................................................................................................... ix
一、前言........................................................................................................................1
1-1 生物膜 ..........................................................................................................1
1-2 生物膜的基本性質 ......................................................................................3
1-2-1 生物膜組成的與性質.........................................................................3
1-2-2 生物膜相態.........................................................................................4
1-2-3 彈性性質.............................................................................................6
1-3 生物膜的研究 ..............................................................................................7
1-4 實驗目的 ......................................................................................................8
二、材料與方法..........................................................................................................10
2-1 實驗材料 ....................................................................................................10
2-2 實驗樣品製備 ............................................................................................11
2-3 實驗儀器 ....................................................................................................13
2-4 X-ray 繞射實驗........................................................................................14
2-5 理論 ............................................................................................................16
2-5-1 X 光繞射.........................................................................................16
2-5-2 Osmotic pressure method................................................................17
2-6 數據處理與分析......................................................................................18
2-6-1 數據處理...........................................................................................18
2-6-2 細胞膜的相態...................................................................................19
2-6-3 六角形相態的結構跟數據分析.......................................................20
2-6-4 數據分析...........................................................................................22
三、結果......................................................................................................................24
3-1 相圖(Phase diagram)..................................................................................25
3-1-1 DOPE 的相圖 ....................................................................................25
3-1-2 分子形狀對 DOPE 相圖的影響 ......................................................27
3-1-3 帶電的脂質對 DOPE 相圖的影響 ..................................................28
3-1-3 蜂毒胜肽對 DOPE 相圖的影響 ......................................................30
3-2 彈性性質 ....................................................................................................30
3-2-1 數據分析...........................................................................................30
3-2-2 脂質混合...........................................................................................36
3-2-3 溫度...................................................................................................38
四、討論......................................................................................................................39
4-1 分子形狀 ....................................................................................................39
4-2 帶電的脂質 ................................................................................................40
4-4 蜂毒胜肽 ....................................................................................................41
4-5 彎曲係數與成分組合 ................................................................................42
4-6 自然曲率半徑與溫度 ................................................................................42
4-7 生物膜的自由能 ........................................................................................43
4-7-1 排列缺陷(packing frustration)與誤差..............................................44
五、結論......................................................................................................................46
參考文獻................................................47
附錄一..........................................................................................................................51

參考文獻

[1] Sylvia S. Mader著，生物學，黃皓陽譯，藝軒圖書，台北，民國八十八年。
[2] Robert T. Murry等著，哈伯氏生物化學，藝軒圖書，翁鵬傑等譯，台北，民國八十六年。
[3] Eddy M. De Robertis著，細胞分子生物學，茂昌圖書，林良平等譯，台北，民國六十九年。
[4] Giangaspero, A., et al. “Amphipathic α-helical peptides. A systematic study
of the effects of structural and physical properties on biological activity.”
Eur. J. Biochem. Vol. 268, pp. 5589 – 5600, 2001.
[5] Huang, H. W., “Action of Antimicrobial Peptides: Two-State Model.” Biochem.
Vol. 39, pp. 8347 – 8352, 2000.
[6] Kim A. Brogden, “Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?” Nature Reviews Microbiology, Vol. 3, pp. 238 – 250, March 2005.
[7] Matanic, V.C.A. and V. Castilla. “Antiviral activity of antimicrobial cationic peptides against Junin virus and herpes simplex virus.” IJAA, Vol. 23, Issue 4, pp. 382 – 389, 2004.
[8] Deng, B., et al. “Correlation of the antibacterial activities of cationic peptide antibiotics and cationic steroid antibiotics.” J. Med. Chem. Vol. 45, pp. 663 – 669, 2002.
[9] Juliette Jouhet, “Importance of the hexagonal lipid phase in biological membrane organization” Front Plant Sci. Vol. 4: 494, 2003.
[10] Z. Chen and R. P. Rand, “The Influence of Cholesterol on Phospholipid Membrane Curvature and Bending Elasticity”, Biophys. J., Vol. 73, pp. 267 –276, 1997.
[11] D.A. Brown and E. London, “Structure and function of sphingolipid-and cholesterol-rich membrane rafts, Journal of Biological Chemistry” Biological Chemistry, Vol. 275, pp. 17221-17224, 2000.
[12] Koynova R, et al. “Transitions between lamellar and non-lamellar phases in membrane lipids and their physiological roles” OA Biochemistry, 1(1):9, Apr 01 2013.
[13] 錢偉長著，彈性力學，亞東書局，台北，民國七十六年。
[14] W. Rawicz, et al. “Effect of Chain Length and Unsaturation on Elasticity of Lipid Bilayers.” Biophys. J., Vol. 79, pp. 328 –339, 2000.
[15] K. Olbrich, et al. “Water Permeability and Mechanical Strength of Polyunsaturated Lipid Bilayers.” Biophys. J., Vol. 79, pp. 321 – 327, 2000.
[16] R. M. Epand, et al. “Role of the Position of Unsaturation on the Phase Behavior and Intrinsic Curvature of Phosphatidylethanolamines”, Biophys. J., Vol. 71, pp.1806 –1810, 1996.
[17] N. Fuller and R. P. Rand, “The Influence of Lysolipids on the Spontaneous Curvature and Bending Elasticity of Phospholipid Membranes”, Biophys. J., Vol. 81, pp.243 –254, 2001.
[18] Habermann, E. “Bee and wasp venoms.”Science 177, pp. 314 - 322 , 1972.
[19] Dempsey, C. E. “The actions of melittin on membranes.” Biochim Biophys. Acta
1031, pp. 143 – 161, 1990.
[20] Lin Yang, “Barrel-Stave Model or Toroidal Model? A Case Study on Melittin Pores”, Biophys. J., Vol. 79, pp. 328 –339, 2000.
[21] Lee, M. T., et al. “Energetics of pore formation induced by membrane active peptides.” Biochemistry, Vol. 43, pp. 3590 – 3599 ,2004.
[22] Christopher B. Stanley and Helmut H. Strey. “Measuring Osmotic Pressure of Poly(ethylene glycol) Solutions by Sedimentation Equilibrium Ultracentrifugation” . Macromolecules, Vol. 36, pp. 6888-6893, 2003.
[23] Wu , Y., et al. “X-Ray Diffraction Study of Lipid Bilayer Membranes Interacting with Amphiphilic Helical Peptides: Diphytanoyl Phosphatidylcholine with
Alamethicin at Low Concentrations.” Biophys. J., Vol. 68, pp. 2361 – 2369
, 1995.
[24] 陳方玉，黃惠文，「以 X 光多片層繞射研究生物薄膜」，科儀新知，18 卷，六期， pp. 22 – 26, 1997.
[25] S. Leikin et al. “Measured Effects of Diacylglycerol on Structural and Elastic Properties of Phospholipid Membranes.” Biophysical J. Vol. 71, pp. 2623 – 2632, November 1996.
[26] Rand, R. P. et al. “Membrane curvature, lipid segregation, and structural transitions for phospholipids under dual-solvent stress.” Biochemistry. Vol. 29, pp. 76 – 87, 1990.
[27] Helfrich, W. “Elastic properties of lipid bilayers: theory and possible experiments.” Z. Naturforsch.28C: pp. 693–703, 1973
[28] Richard M. Epand. “High sensitivity differential scanning calorimetry of the bilayer to hexagonal phase transitions of diacylphosphatidylethanolamines” Chemistry and Physics of Lipids. Vol. 36, pp. 387-393, March 1985
[29] G. L. Kirk and S. M. Gruner. “Lyotropic effects of alkanes and headgroup composition on the L03B1 -HII lipid liquid crystal phase transition: hydrocarbon packing versus intrinsic curvature” J. Physique, Vol. 46, pp. 761-769, 1985.
[30] Ming-Tao Lee. et al. “Many-Body Effect of Antimicrobial Peptides: On the Correlation Between Lipid’s Spontaneous Curvature and Pore Formation” Biophysical Journal, Vol. 89, pp. 4006–4016, December 2005.
[31] Terwilliger, T. C. et al.” The structure of melittin in the form I crystals and its implication for melittin’s lytic and surface activities.”Biophys. J., Vol. 37, pp. 353–361, 1982.
[32] GCShearman et al. .” Inverse lyotropic phases of lipids and membrane
curvature” J.Phys. , Vol. 18, pp.S1105–S1124, 2006.

指導教授

李明道(Ming-Tao Lee)

審核日期

2015-1-23

推文