博碩士論文 102226059 詳細資訊

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姓名 徐鈺(Yu John Hsu)  查詢紙本館藏   畢業系所 光電科學與工程學系
論文名稱 微投影光學切片超光譜顯微術
(DLP-Based Hyperspectral Imaging via Optical Sectioning Microscopy)
★ 非反掃描式平行接收之雙光子螢光超光譜顯微術★ 以二次通過成像量測架構及降低誤差迭代演算法重建人眼之點擴散函數
★ LASER光源暨LED在老鼠毛生長的低能量光治療比較分析★ 應用線狀結構照明提升雙光子顯微鏡解析度
★ 以同調結構照明顯微術進行散射樣本解析度之提升★ 掃描式二倍頻結構照明顯微術
★ 小貓自泵相位共軛鏡於數位光學相位共軛與時間微分之研究★ 鏡像輔助斷層掃描相位顯微鏡
★ 以數位全像術重建多波長環狀光束之研究★ 相位共軛反射鏡用於散射介質中光學聚焦之研究
★ 雙光子螢光超光譜顯微術於多螢光生物樣本之研究★ 倍頻非螢光基態耗損超解析之顯微成像方法
★ 葉綠素雙光子螢光超光譜影像於光合作用研究之應用★ 雙光子掃描結構照明顯微術
★ 使用結構照明顯微術觀察活體小鼠毛囊生長週期之變化★ 一次性多角度漫射光譜量測系統
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摘要(中) 超光譜顯微術利用不同物質、組織有不同放射光譜的特性,最開始用於對地球表面上的觀測,後陸續衍伸到多種領域,並同時在生醫醫學上大量應用。其中,擁有光譜高解析度的為空間掃描的系統,又可分為點掃描及線掃描這兩種掃描架構。點掃描普遍的缺點為取樣時間過久,線掃描雖然降低了取樣時間,但犧牲了長軸上的解析度。本研究將線掃描超光譜顯微術與擁有光學切片能力的結構照明顯微術結合,利用結構照明提升長軸上的解析度,成功架設了光學切片超光譜顯微系統。
摘要(英) Hyperspectral imaging makes use of the intrinsic spectral property, in which different materials and tissues solely possess their own emission spectrum. It was originally utilized in remote sensing, and was then developed for a wide variety of fields, including the biomedical field. In hyperspectral imaging systems, high spectral resolution can be achieved by spatial scanning methods which can be further divided into line-scanning approaches and point-scanning approaches. The common disadvantage of the point-scanning approach is its rather long acquisition time, whereas was reduced in line-scanning approaches, but it came with the price of sacrificing its spatial resolution in its long-axial. In this research, line-scanning hyperspectral imaging system is combined with structured illumination to improve the spatial resolution, successfully constructing a hyperspectral imaging system via optical sectioning microscopy.
In this thesis, the hyperspectral imaging system was applied to acquire the fluorescence hyperspectral data of specimens. Utilizing the one-dimensional spectral and two-dimensional spatial information, in addition with the linear unmixing process, the aim is to solve the common crosstalk problem in spectral imaging. With specimens of pollens and multiple stained mouse hair follicles, the imaging results demonstrated that the system can effectively overcome the crosstalk problem, and successfully analyze the constituents of the specimen.
關鍵字(中) ★ 超光譜
★ 光學切片
★ 顯微術
★ 微投影機
★ 結構照明
★ 線性分析
關鍵字(英) ★ Hyperspectral Imaging
★ Optical Sectioning
★ Microscopy
★ Linear Unmixing
論文目次 摘要 v
Abstract vi
Index viii
Index of Figures x
Chapter One: Introduction 1
1.1 Hyperspectral Imaging Overview 1
1.2 Spectral Scanning Approach 6
1.3 Spatial Scanning Approach 7
1.3.1 Point-scanning Method 9
1.3.2 Line-scanning Method 11
1.4 Motivation 12
Chapter Two: Theories 14
2.1 Structured Illumination Microscopy (SIM) 14
2.2 Linear Unmixing 21
Chapter Three: System Configuration 24
3.1 System Configuration 24
3.1.1 The Digital Light Processing (DLP) Projector 28
3.1.2 The Detector 31
3.1.3 The Wavelength Selection Components 32
3.2 The Spectrometer 34
3.2.1 The Spectral Dimension 34
3.2.2 The Spatial Dimension 38
3.3 System Spectrum Calibration 39
3.3.1 The Pixel-Number-to-Wavelength Conversion 39
3.3.2 The Spectral Resolution 42
Chapter Four: Experimental Results 44
4.1 Optical Sectioning Imaging of Mesophyll Cells 44
4.2 Spatial Resolution of Hyperspectral Imaging 46
4.3 Hyperspectral Imaging of Pollens 48
4.3.1 Catharanthus Roseus 48
4.3.2 Specimen Preparation 48
4.3.3 System Parameters Settings 49
4.3.4 Hyperspectral Imaging Results 50
4.4 Hyperspectral Imaging and Spectral Unmixing of Multiple Fluorescence Stained Mouse Skin 53
4.4.1 Mouse Hair Follicle Bulge Stem Cells 53
4.4.2 Sample Preparation 55
4.4.3 Sectioned Samples 56
4.4.4 Whole Mount Specimen 64
Chapter Five: Conclusion 68
References 69
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指導教授 陳思妤 審核日期 2016-8-26
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