博碩士論文 102323102 詳細資訊




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姓名 黃彥倫(Yan-Luen-Huang)  查詢紙本館藏   畢業系所 機械工程學系
論文名稱 多重微流體晶片機械應力刺激細胞培養之研究
(Multiple microfluidic chips mechanical stress stimulation of cell culture)
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摘要(中) 癌症目前在全世界死亡之首,其特徵癌細胞不受控制的繁殖,藉著血液、淋巴系統的循環,散播到身體其他部位,形成癌症的轉移。目前治療方法有腫瘤切除術、化學治療及放射性治療等治療方式,近年來研究發現機械力刺激細胞會影響細胞的生長因子,希望以機械力刺激癌細胞可以找到治療的機制,而微流體晶片進行生物檢測或分析具有降低人工操作的實驗誤差、自動化控制系統穩定性、降低耗能及樣品用量、時間等優點。
本研究以微流體晶片作為進行剪應力、正向應力刺激癌細胞的裝置,此裝置以設計出多項不同剪應力、正向應力刺激癌細胞,結構可將其區分成上氣閥層與下層流道層的剪、正向應力實驗晶片,氣閥層以負光阻製作出高深寬比的微結構,流道層以正光組製作出圓角的微結構,圓角有利於閥門元件能夠緊密關閉流道,利用螢光染液證實閥門的緊密度,先將癌細胞種植於下層的微流道內部腔體進行多重應力刺激系統的整合在微流體晶片上。再利用Lab view程式進行自動化控制氣閥門,及正向力氣室配合不同的時間和壓力進行刺激。
摘要(英) Cancer is currently the world′s leading cause of death, in recent years, research shows that mechanical stress are also found to have some effects to the cells . The side effects by chemo or radiation therapy may be reduced by the physical treatment assistance. We using microfluidic chip to physically and chemically stimulate cancer cells to study the mechanical stress effects to the cancer cells. Microfluidic chip for bio-detection or analysis has the advantages of reducing the manual error, automatic control system stability, reducing energy consumption and sample consumption, time, etc.

In this study, microfluidic chips were used as a device for shear stress and positive stress stimulation of cancer cells. The device to design a number of different shear stress, positive stress to stimulate cancer cells. The structure can be divided into the upper valve layer and the lower layer of the flow channel shear, positive stress test chip. The valve layer to negative light resistance to make a high aspect ratio of the microstructure. The flow path layer is made of round corners by the positive light group. So cancer cells are implanted in the lower chamber of the microchannel cavity for the integration of multiple stress stimuli on microfluidic chips, and then use the Lab view program to automatically control the gas valve, and the positive force chamber with different time and pressure to stimulate.
關鍵字(中) ★ 癌細胞
★ 機械應力刺激
★ 微流體
關鍵字(英) ★ cancer cell
★ Mechanical stress
★ Microfluidics
論文目次 摘要 i
Abstract ii
誌謝 iii
目錄 iv
圖目錄 vii
表目錄 ix
第一章 緒論 1
1.1前言 1
1.2研究動機與目的 3
1.3論文架構 4
第二章 文獻回顧 5
2.1微流體文獻 5
2.2機械應力刺激細胞文獻 8
2.3利用微流體晶片機械應力刺激細胞文獻 10
第三章 實驗原理與實驗步驟 13
3.1實驗材料與藥品 14
3.2 實驗器材 15
3.3 微流道晶片製程 17
3.3.1製作光罩 17
3.3.2黃光製程 18
3.3.3 PDMS翻轉印模製程 21
3.3.4氧電漿接合 22
3.4 Lab VIEW程式設計 23
3.4.1人機介面(GUI) 24
3.5實驗系統架設 25
3.5.1微流體晶片 26
3.5.2程式控制系統 27
3.5.3環境控制系統 28
3.5.4光學觀測系統 28
3.6細胞植入晶片實驗流程 29
3.6.1實驗前置作業 29
3.6.2細胞植入晶片 30
3.6.3機械刺激細胞 32
3.6.4染色步驟 34
3.6.5細胞計數與影像分析 35
第四章 結果與討論 36
4.1晶片設計 36
4.2剪應力與正向應力刺激流程 39
4.2.1剪應力與正向應力實驗設定 42
4.3微流體晶片種植細胞 42
4.4鼠結腸癌細胞受到機械應力刺激後之結果 46
4.4.1 剪應力刺激鼠結腸癌細胞的影響 48
4.4.2 正向應力刺激鼠結腸癌細胞的影響 54
第五章 結論與未來展望 56
5.1結論 56
5.2未來展望 57
參考文獻 58
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指導教授 曹嘉文(Chia-Wen-Tsao) 審核日期 2017-1-24
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