博碩士論文 103324034 詳細資訊




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姓名 楊淳行(Yang Chun-Hsing)  查詢紙本館藏   畢業系所 化學工程與材料工程學系
論文名稱 探討以PDMS海綿萃取發酵系統連續生產丁醇之研究
(Continuous Butanol Production by using PDMS Sponge Extractive Fermentation)
檔案 [Endnote RIS 格式]    [Bibtex 格式]    至系統瀏覽論文 (2018-7-1以後開放)
摘要(中) 本研究為利用Polydimethylsiloxane海綿對Clostridium acetobutylicum發酵液進行連續萃取發酵,並探討其效益及可行性。Clostridium acetobutylicum進行ABE發酵時,若其發酵液中丁醇濃度達到8 gL-1將使菌體進入內孢子狀態,不再生產丁醇,產生嚴重的抑制效應,進而導致失活。因此藉由將發酵液多次以蠕動幫浦引流通過裝有PDMS海綿之容器的方式,目的在於降低發酵液的丁醇濃度,並討論於適當時機下替換新鮮發酵液,以達提升總丁醇產率與產量之目的。
本研究實驗利用PDMS海綿,用以萃取抑制物,於菌體發酵達48小時後進行丁醇萃取,發酵液之工作體積為150 ml以蠕動幫浦抽出並注入裝有重量為10 g的PDMS海綿之容器中,最後回流至厭氧發酵罐,萃取時間為15分鐘。後於第60小時開始間隔24小時將50 mL發酵液抽出替換為同等體積之新鮮液態培養基。與控制組相比,丁醇總產量由1.725 g提升至7.89 g,而總產率由0.087 gL-1 hr-1略為成長至0.092 gL-1 hr-1,並使發酵時間從108小時延長至192小時,足見PDMS海綿與連續萃取發酵製程不僅可減緩其失活速度,並因海綿降低發酵液中的丁醇濃度,排除了抑制效應,而使菌體快速生長,以利提升丁醇之產量與產率,故利用PDMS海綿作為萃取物之研究,值得深入探討。
摘要(英) In this research, we extract butanol from the continuous fermentation of Clostridium acetobutylicum with Polydimethylsiloxane (PDMS) sponge , and discuss its effect and feasibility. As the concentration of butanol reach about 10 gL-1 in ABE fermentation, Clostridium acetobutylicum will be inhibited by its product, butanol, and lose its activation. It will become into a state of endospore and stop to product butanol. Thus, we will reach the target of promoting the productivity of butanol by circulating the broth into PDMS sponge system by peristaltic Pump and refresh the broth in suitable moment.
PDMS sponge is used to extract the inhibitor in this research. When the fermentation reach 48 hours, it will be extracted butanol every 12 hours. The volume of working fluid of the broth is 150 mL. It will be inject into the system including 10 g PDMS sponge for 15 minutes by peristaltic Pump and reflow. It will be refresh the 50 mL broth into liquid medium in same volume. In contrast to the control, the total productivity of butanol is promoted from 1.725 to 7.89 g. The fermentation period is expanded from 108 to 192 hours. Because of PDMS sponge and the continue extractive fermentation, It will not only be slow the losing of the activation down, but also cut the inhibit effect off. And the total productivity of butanol is increased.
Therefore, the process feasibility of using PDMS hydrophobic sponge as an extractant, is worth in-depth study.
關鍵字(中) ★ PDMS海綿
★ 萃取發酵
★ 丁醇
★ PDMS
★ 發酵
★ 萃取
關鍵字(英)
論文目次 目錄

摘要 ----------------------------------------------------------------------------------- i
Abstract ------------------------------------------------------------------------------- ii
致謝 ----------------------------------------------------------------------------------- iii
目錄 ----------------------------------------------------------------------------------- iv
圖目錄 -------------------------------------------------------------------------------- viii
表目錄 -------------------------------------------------------------------------------- xi

第一章 緒論
1.1 研究動機 ------------------------------------------------------------------ 1
1.2 研究目的 ------------------------------------------------------------------ 3

第二章 文獻回顧
2.1 再生能源
2.1.1 再生能源介紹 -------------------------------------------------- 4
2.1.2 生質能源發展及應用 ----------------------------------------- 5


2.2 生質丁醇
2.2.1 丁醇介紹-------------------------------------------------------- 6
2.2.2 丁醇生產方式-------------------------------------------------- 7
2.3 發酵法
2.3.1 ABE發酵介紹 ------------------------------------------------ 9
2.3.2 Clotridium acetobutylicum 菌種介紹 -------------------- 10
2.3.3 ABE發酵代謝途徑 ----------------------------------------- 13
2.4 抑制效應
2.4.1 抑制效應的實例 --------------------------------------------- 15
2.4.2 抑制效應的改善方法----------------------------------------- 15
2.4.3 各種改善方法之比較 ----------------------------------------18
2.5 Polydimethylsiloxane
2.5.1 PDMS 基本性質 ---------------------------------------------19
2.5.2 PDMS海綿基本性質 --------------------------------------- 22
2.6 吸附基本原理
2.6.1 吸附現象------------------------------------------------------- 26
2.6.2 吸附種類 ------------------------------------------------------ 27




第三章 材料與方法
3.1 實驗規劃 ------------------------------------------------------------------ 30
3.2 實驗材料
3.2.1 實驗菌株 ------------------------------------------------------ 31
3.2.2 實驗藥品 ------------------------------------------------------- 32
3.3 實驗方法
3.3.1 菌種保存 ------------------------------------------------------ 34
3.3.2 培養基組成 ----------------------------------------------------35
3.3.3 接種菌體培養 -------------------------------------------------38
3.3.4 PDMS海綿製備 ----------------------------------------------39
3.3.5 萃取式發酵 ----------------------------------------------------40
3.4 分析方法
3.4.1 菌重濃度測定 -------------------------------------------------- 41
3.4.2 葡萄糖殘量分析 ----------------------------------------------- 42
3.4.3 ABE溶液濃度分析 ------------------------------------------- 44
3.5 實驗數據之各項參數介紹 --------------------------------------------48


第四章 實驗結果討論
4.1 PDMS海綿基本性質
4.1.1 重複使用對PDMS海綿吸附能力的影響---------------- 49
4.1.2 PDMS 海綿對葡萄糖溶液之吸附能力------------------ 51
4.1.3 PDMS 海綿對各有機溶劑之吸附能力------------------ 52
4.2 利用PDMS海綿進行萃取發酵
4.2.1 控制組實驗 -------------------------------------------------- 53
4.2.2 葡萄糖濃度對菌體發酵產生的影響 -------------------- 54
4.2.3 以海綿萃取的時機對於發酵產生的影響 -------------- 56
4.2.4 以海綿進行連續萃取的時機對發酵的影響 ------------59
4.2.5 Feed batch饋料之成分對發酵的影響 ------------------ 62
4.2.6 Feed batch添加時機對發酵的影響 --------------------- 66
4.2.7 連續式發酵的時機與成分對發酵的影響 -------------- 70
4.2.8 連續萃取發酵-------------------------------------------------73
第五章 結論與建議
5.1 結論 ----------------------------------------------------------------------- 75
5.2 建議 ----------------------------------------------------------------------- 76

第六章 參考文獻 ------------------------------------------------------------------78
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指導教授 徐敬衡(Chin-Hang Shu) 審核日期 2016-8-24
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