博碩士論文 104226055 詳細資訊




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姓名 羅仁瓏(Jen-Long Lo)  查詢紙本館藏   畢業系所 光電科學與工程學系
論文名稱 以氮化物表面電漿結構研製的生醫感測微晶片
(Microarray biochips fabricated with Nitride-based Surface Plasmon Resonance)
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摘要(中) 表面電漿式的蛋白質微晶片陣列具有免螢光、免清洗、多工、即時等優點,過去三十年來,表面電漿感測元件已從實驗室走向商品,成為生物醫學檢測的一項利器。目前的表面電漿生醫感測系統多採用克式結構(Kretschmann configuration),以稜鏡產生高動量入射波,搭配鍍有金屬的載玻片及複雜的光學追蹤系統,才能偵測到微弱的光訊號變化。然而稜鏡不只體積大、價格貴,與載玻片之間也有光耦合不易的問題。
在本研究中,我們以高折射率的氮化鎵磊晶層取代稜鏡,並在表面的金屬層產生表面電漿效應,不但能簡化感測元件的設計,還能增加表面電漿的發光強度。此外,我們採用的氮化銦鎵量子井,可做為表面電漿效應的增益層(gain medium),具備訊號放大效果,能偵測到更低濃度之檢體,因而提升系統敏感度與解析能力。並可藉由調整氮化銦鎵量子井的磊晶結構,優化量子井與表面電漿偏振子(surface plasmon polaritons)之間的耦合效率,以產生更強之表面電漿共振。
藉由檢測酒精、抗原Protein G與人體抗體IgG三種溶液以驗證系統可行性。量測檢果顯示:酒精溶液其敏感度為8×〖10〗^(-5) (RIU^(-1)),人體抗體IgG溶液檢測極限(Limit of detection)可達1.89nM。
摘要(英)
Comparing with conventional biosensing techniques, surface plasmon resonance (SPR) biosensors allow label-free, wash-free, multi-analyte and real-time measurement, which can significantly expedite the assay process. However, most of current SPRi devices attain the SPR effect through the Kretschmann configuration, i.e. a complex involving a prism coupler, a glass slide, the refractive-index-matching oil, and intricate optical tracking components. In specific, a prism coupled with an Au-coated glass is usually required to attain the SPR effect through total internal reflection. The prism-glass complex is expensive, and often encounters the difficulty in optical coupling between the two components.
In this project, we built a nitride-based SPR proteome microarrays. The SPR structure comprises a single GaN/InGaN/GaN quantum well coated with a thin Ag layer. Biomolecular interactions are detected by the SPR effect induced at the GaN/Ag interface, where a minute change in refractive index can lead to measurable variation of the emission intensity from the quantum well. The InGaN quantum wells are of many inherent properties that are matchlessly suitable for SPRi biosensing, including the waveguide-like behaviour, the chemical and physical inertness, the TM-(or p-) polarized wave, and most importantly: the gain effect. These favorable properties not only simplify the sensing architecture by eliminating all the aforementioned apparatuses (prism, glass slide, index-matching oil, optical tracking components, etc.), but also pave a new route to push sensing performances via the prolonged propagation lengths of SP polaritons. More importantly, we show that the multi-mode characteristic of the quantum wells lead to an exponentially improved sensitivity/resolution upon the increase of surface refractive index, unlike the linear response exhibited by current SPR biosensors. Using human IgG, we demonstrate the detection limit of 1.89 nM with the nitride-based SPR biosensor.
關鍵字(中) ★ 氮化物
★ 表面電漿
關鍵字(英) ★ Nitride
★ Surface Plasmon
論文目次
中文摘要 I
ABSTRACT II
誌謝 IV
目錄 V
圖目錄 VII
表目錄 IX

第一章、緒論
1-1 前言1
1-2 氮化物材料結構與特性 5
1-3 表面電漿生醫感測晶片的優勢 6
1-4 研究動機與章節架構 9
第二章、實驗方法、製程與儀器
2-1 表面電漿原理 11
2-2 表面電漿色散曲線 16
2-3 表面電漿共振現象 20
2-4 有機金屬化學氣相沉積儀簡介 22
2-5 點片機MARTARRAYERTM136 23
2-6 磊晶片結構與製作流程 24
2-7 藥品介紹 25
第三章、分析與討論
3-1 表面電漿產生效果驗證 26
3-2 食鹽水反應 27
3-3 酒精溶液反應 30
3-4 抗原Protein G 35
3-5 IgG之反應 37
3-4 微晶片陣列 39
第四章、結論與未來發展
4-1 結論 41
4-2 未來發展 42
參考文獻 43
參考文獻
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指導教授 賴昆佑(Kun-Yu Lai) 審核日期 2017-7-12
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