基於半胱氨酸的水凝膠與銅離子結合以抵抗USA300耐甲氧西林金黃色葡萄球菌作為有效的傷口敷料

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姓名

黃詠琪(Yung-Chi Huang) 查詢紙本館藏

畢業系所

生命科學系

論文名稱

基於半胱氨酸的水凝膠與銅離子結合以抵抗USA300耐甲氧西林金黃色葡萄球菌作為有效的傷口敷料
(cysteine-based hydrogel bind with copper ions to against USA300 Methicillin-Resistant Staphylococcus aureus as effective wound dressings)

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摘要(中)

先前的研究中發現，耐甲氧西林金黃色葡萄球菌（金黃色葡萄球菌）（MRSA）是膿性皮膚和軟組織感染最常見的原因。儘管抗生素已被廣泛用於治療MRSA的皮膚感染，但是不適當地使用抗生素會有非常大的風險產生具有抗藥藥性金黃色葡萄球菌。因此我們開發了可以吸收和釋放銅離子的水膠，一種具有抑制USA300（一種社區型MRSA）生長能力的半胱氨酸水凝膠。傅里葉變換紅外光譜（FTIR）分析的結果表明以半胱氨酸為基材的水膠與銅離子間具有相互作用。在應用方面，具有半胱氨酸水膠與銅結合到ICR小鼠背部皮膚上的USA 300感染皮膚上顯著增強傷口癒合，阻礙USA300的生長，並減少促炎症巨噬細胞炎症蛋白2- α（MIP-2）細胞因子。我們的研究成功完成一種水膠具有與銅離子結合的功能，並可抑制微生物的生長，做為一種新式的傷口敷料。

摘要(英)

The methicillin-resistant Staphylococcus aureus (S. aureus) (MRSA) is reported as the most common cause of purulent skin and soft tissue infections. Although antibiotics have been widely used for treatment of skin infection of MRSA, but the inappropriate use of antibiotics runs a considerable risk of generating resistant S. aureus. Here we develop a cysteine-capped hydrogel which can absorb and release copper, an ion with the capability of suppressing the growth of USA300, a community-acquired MRSA. Results from the analysis of fourier transform infrared spectroscopy (FTIR) indicate the interaction of a cysteine-capped hydrogel with copper. Topical application of a cysteine-capped hydrogel binding with copper onto a USA300-infected skin wound in the dorsal skin of ICR mice significantly enhance the wound healing, hinder the growth of USA300, and reduce the production of pro-inflammatory macrophage inflammatory protein 2-alpha (MIP-2) cytokine. Our work demonstrates a newly designed hydrogel which conjugates a cysteine molecule for copper binding. The cysteine-capped hydrogel potentially can chelate various antimicrobial metals as a novel wound dressing.

關鍵字(中)

★ 水膠
★ 銅離子
★ 傷口敷料
★ 抗菌
★ 釋放

關鍵字(英)

★ hydrogel
★ copper ion
★ wound dressing
★ antibacterial
★ release

論文目次

摘要 VI
ABSTRACT VII
謝誌 VIII
目錄 IX
圖目錄 XII
表目錄 XIV
第一章、緒論 1
1-1抗耐甲氧西林金黃色葡萄球菌 (METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS，MRSA) 1
1-2皮膚 2
1-2-1皮膚的結構及功能 2
1-2-2皮膚傷口癒合過程 4
1-3金屬離子-銅(copper) 8
1-4敷料(DRESSING) 9
1-4-1敷料之演進 9
1-4-2敷料的使用與類型 [39] 11
1-5水膠(HYDROGEL) 12
1-5-1水膠的簡介 12
1-5-2智慧型水膠 [46] 13
1-6帶有硫醇基分子的天然胺基酸兩性雙離子 14
1-7金屬離子與高分子材料螯合 16
第二章、研究目標 18
第三章、實驗方法 19
3-1材料 19
3-2實驗儀器 20
3-3半胱氨酸甲基丙烯酸酯 (CYSTEINE METHACRYLATE，CYSMA)/氯化銅水膠製備流程 21
3-3-1半胱氨酸甲基丙烯酸酯 (Cysteine Methacrylate，CysMA)合成 21
3-3-2半胱氨酸甲基丙烯酸酯/氯化銅水膠 21
3-3-3甲基丙烯酸3-（丙烯酰氧基）-2-羥丙酯/氯化銅水膠 22
3-4 FTIR光譜分析 22
3-5水膠釋放銅離子 22
3-6抑菌圈試驗 23
3-8傷口感染動物實驗模式 23
3-8-1傷口大小 23
3-8-2 Bacterial counting 24
3-8-3 MIP-2 24
第四章、結果與討論 26
4-1半胱氨酸甲基丙烯酸酯 (CYSTEINE METHACRYLATE，CYSMA)單體的製備 26
4-2 水膠的表觀 27
4-2-1 CysMA 水膠 27
4-2-2 MA水膠 28
4-3 水膠的吸收及釋放 29
4-3-2 CysMA水膠吸收及釋放 29
4-3-2 MA水膠吸收及釋放 29
4-4FTIR 分析 33
4-5抗菌試驗 35
4-6 傷口感染動物實驗模式 38
4-6-1 傷口面積測定 38
4-6-2 Bacterial counting 40
4-6-3酵素免疫分析法 42
第五章、結論 43
第六章、參考文獻 45

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指導教授

黃俊銘(Chun-MIng Huang)

審核日期

2017-8-23

推文