博碩士論文 104821016 詳細資訊




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姓名 温栩綸(Hsu-Lun Wen)  查詢紙本館藏   畢業系所 生命科學系
論文名稱
(Classification of powdery mildews on ornamental plants in northern Taiwan)
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摘要(中) 白粉菌是世界上很常見的植物病原菌之一,會引起植物產生白粉病的真菌。感染近一萬種的被子植物,白色粉末病斑會出現在葉、莖、花和果實上,受到感染的植物葉片通常會扭曲、泛黃凋落。目前台灣所發現的白粉菌多是無性世代,所以需要使用ITS rDNA序列進行分子鑑定以更明確的鑑定物種。樣品鑒定出4個屬9個種的白粉菌,當中發現了三個白粉菌全球宿主新紀錄(Erysiphe lespedezae on Desmodium caudatum, Podosphaera xanthii on Mazus pumilus, Podosphaera xanthii on Sphagneticola trilobata),三個白粉菌台灣宿主新紀錄(Erysiphe sedi on Kalanchoë spp., Golovinomyces biocellatus on Salvia sp., Podosphaera xanthii on Bidens pilosa)。
摘要(英) Powdery mildews belong to the most common plant pathogens in the world. Infecting nearly 10,000 species of angiosperms, white powdery mildew lesions appear on leaves, stems, flowers, and fruits, and infected plant leaves are often distorted and discolored. At present, most of the powdery mildews in Taiwan are found as asexual stage with few morphological differences between species, so that ITS rDNA sequences are needed for molecular identification of the species. In this study, we collected 9 species of powdery mildews with relationship to four teleomorph genera (Erysiphe, Golovinomyces, Podosphaera, Sawadaea), including three new host records worldwide (Erysiphe lespedezae on Desmodium caudatum, Podosphaera xanthii on Mazus pumilus, Podosphaera xanthii on Sphagneticola trilobata), and three new host records for Taiwan (Erysiphe sedi on Kalanchoë spp., Golovinomyces biocellatus on Salvia sp., Podosphaera xanthii on Bidens pilosa).
關鍵字(中) ★ 白粉菌 關鍵字(英) ★ powdery mildews
論文目次 中文摘要………………………………..…….....…….……….………….…………….Ⅰ
Abstract…………………………….……………...……….………………….……….Ⅱ
誌謝…………………………………………………...…………………………….….Ⅳ
Contents…………………………………….…….…………………….………….….Ⅴ
Figures………………………………...…………………………………….……….Ⅷ
Table………………………………………………….……………...……………..….Ⅸ
1.Introduction………………..………………………….…………………….……….1
1.1 Life cycle………………………………...……………………….………….1
1.2 History of research about Erysiphales…………………………....…….3
1.3 Aim……………………………………………….………………….……….3
2. Materials and Methods…………………………….……………………..……….5
2.1 Field collection…………………………………………………………..….5
2.2 Preservation of fungal specimen…………………………..…………….6
2.3 DNA isolation………………………………………………………….…….6
2.3.1 Tissue Dissociation……………………………..………..……….6
2.3.2 Lysis…………………………………………………..………….….7
2.3.3 DNA Binding…………………………………….…..…….……….8
2.3.4 Wash………………………………………….....…………….…….9
2.3.5 DNA Elution……………………………...….………….………...10
2.4 PCR…………………………………………………………………..….….10
2.4.1 Material…………………………………….…….………….…….10
2.4.2 Protocol for PCR…………………………………….…..……….11
2.5 Agarose gel electrophoresis………………………………..……..…….13
2.6 DNA sequencing……………………………………………...…….…….13
2.7 Identification………………………………………..……………….…….13
2.7.1 Morphological identification……………….………..………….13
2.7.2 Molecular identification ……………………………………….14
3. Results…………………………………………………………………….……….15
3.1 General results……………………………....………………..………….15
3.1.1 Erysiphe lespedezae U. Braun & R.Y. Zheng on Desmodium
caudatum…….…………...……………….……….............…...16
3.1.2 Erysiphe sedi U. Braun on Kalanchoë sp……………….……18
3.1.3 Golovinomyces biocellatus V.P. Heluta on Salvia sp. ….…..20
3.1.4 Podosphaera xanthii U. Braun & Shishkoff on Bidens
pilosa………………………...............……………….………….22
3.1.5 Podosphaera xanthii U. Braun & Shishkoff on Cucurbitaceae
sp. .…................................................................................24
3.1.6 Podosphaera xanthii U. Braun & Shishkoff on Mazus
pumilus. ….........................................................................25
3.1.7 Podosphaera xanthii U. Braun & Shishkoff on Sphagneticola
trilobata..............................................................................27
3.1.8 Podosphaera xanthii U. Braun & Shishkoff on Zinnia
elegans. ……..…….…………………………………….……….29
3.1.9 Sawadaea cf. polyfida R.Y. Zheng & G.Q. Chen on Acer sp.
…………………………………………...……..………….……...31
4. Discussion…………………………………………………………………...…….33
4.1 Species identification…………………………………………………….33
4.1.1 Vital taxonomy………………..………………………….……….33
4.1.2 Microscopical characteristics …………..…………………….33
4.2 Host range………………………………………..………………………..34
5. References……………………………………..……...………………………….37
6. Appendices……………………...…………………….…………………………..52
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指導教授 羅南德 王健家(Roland Kirschner Chien-Chia Wang) 審核日期 2019-6-21
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