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姓名	翁慈珮(Chi-Pei Weng)  查詢紙本館藏	畢業系所	生命科學系
論文名稱	阿拉伯芥熱誘導性狀突變株R45之基因定位及HSP40參與植物耐熱機轉之探究 (Gene mapping of Arabidopsis heat insensitive R45 mutant and the study of the role of HSP40 in heat-tolerance mechanism)
相關論文	★ 阿拉伯芥突變種(hit1)之位址定位 ★ 阿拉伯芥之HIT1蛋白質為酵母菌Vps53p之對應物且能影響植物對高溫及水份逆境之耐受性 ★ 阿拉伯芥繫鏈同源蛋白質HIT1對頂端生長之影響及熱耐受基因HIT2之遺傳定位 ★ 阿拉伯芥hit3遺傳位址定位與HIT1啟動子分析 ★ 利用基因功能活化法研究阿拉伯芥乙烯生合成之調控機制 ★ 阿拉伯芥突變種hit2之位址定位 ★ 利用化學遺傳法研究阿拉伯芥 revert to eto1 41 (ret41) 之功能研究 ★ 阿拉伯芥hit3和et突變種之生理定性及其基因定位 ★ 阿拉伯芥囊泡繫鏈因子HIT1在逆境下維持內膜完整性之探討與ret8之基因定位 ★ 阿拉伯芥HS29之基因定位及ET參與植物耐熱機轉之探究 ★ 阿拉伯芥中藉由核運輸接受器HIT2/XPO1A進行核質間運輸以促使植物耐受高溫逆境之專一分子的探索研究 ★ 阿拉伯芥hs49與78hs突變株之生理定性及其耐熱基因定位 ★ 阿拉伯芥HIT4為不同於MOM1的新調節方式調控熱誘導染色質重組並在各個植物生長發育轉換時期表現 ★ 阿拉伯芥hit4逆轉株r13及r34之基因定位與r34耐熱機轉之探究 ★ 蛋白質法尼脂化修飾參與植株耐熱反應 ★ 探討ETO1-LIKE1(EOL1)及EOL2參與阿拉伯芥幼苗光形態發育之功能
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摘要(中)	由於植物無法自由移動，是以必須演化出許多逆境抵禦機制，以因應瞬息萬變的生長環境。近年來溫室效應導致全球氣溫上升，而高溫對植物而言是環境中主要非生物逆境之一。高溫逆境會影響農作物的正常生長與發育、劣化品質。嚴重的話會導致植物的死亡，減損產量。是以了解植物抵抗高溫逆境的相關機轉，再運用這些知識來提升農作物對高溫的耐受力，以因應未來糧食供應之所需，有其必要性，也是本研究的出發點。在此，我們運用前向式遺傳學的策略，篩選出對熱敏感的突變植株，藉此找出植物耐受熱逆境的必要基因，並解析植物耐熱機制。利用此策略所篩選出的heat-intolerant 5（hit5），如篩選條件，對持續性溫和高溫敏感，然對短期熱休克則有更強的耐受力。在基因定位後，確認hit5的熱敏感性狀，是因ENHANCED RESPONSES TO ABA 1 （ERA1）基因發生突變所引起。從前人研究得知，此基因表現後所製造出的產物，為植物內蛋白質法尼酯轉移酶（Protein Farnesyltransferase, PFT）的β次單元。PFT由α及β兩個次單元所組成，催化反應為將一個含有15個碳鏈的脂質，接到特定的蛋白質的C端，屬於後轉錄修飾的一種形式。如同其名，ERA1基因突變會使得植株對於ABA敏感，而ABA為一已知能促使植物耐受逆境的重要賀爾蒙。為了釐清hit5的熱相關性狀與ABA調節是否有關，本研究進行了外加ABA及抑制ABA生合成影響hit5熱相關性狀的實驗，證實hit5熱相關性狀不受ABA調節。據此，推測另有其它目前未知的因子，作用在HIT5下游，分別促使植物因應不同形式的熱逆境。另根據文獻報導，若干植物分子量40 kD熱休克蛋白質家族成員，為PFT的受質。經實驗驗證，其中的一個成員的確有參與在HIT5所調節的熱逆境反應。與此同時，本研究再次以前向式遺傳學的策略，透過篩選能恢復hit5熱相關性狀回復成野生型之突變株，來找尋其他參與在HIT5調節熱逆境反應的遺傳因子，以助於更加釐清HIT5所調控的植物熱反應機制。
摘要(英)	Plants are sessile organisms and thus vulnerable to the adverse environment in which they live. High temperature is one of the major environmental adversities that can decrease crop yields. To understand how plants resist high temperature stress, which is essential for developing heat-tolerant crops, we used a forward genetic approach to screen for EMS-mutagenized Arabidopsis mutants that are more thermosensitive than the WT. heat-intolerant 5 (hit5) was therefore isolated because prolonged heat incubation at 37°C for 4 d was lethal for hit5 but not for the WT seedlings. However, hit5 mutant has better ability to tolerance sudden heat shock treatment at 44°C for 40 min than WT. Map-based cloning revealed that HIT5 is ERA1 gene that encodes the β subunit of the protein farnesyltransferase (PFT). Protein farnesylation is a post-translational modification known to mediate abscisic acid (ABA)-regulated drought tolerance in plants. To verify the role of ABA in HIT5-mediated heat stress response (HSR), exogenous application of ABA or ABA synthesis inhibitor were proformed and the results indicated that these treatments did not affect the sensitivity of hit5 to either prolonged heat incubation or sudden heat shock treatment. These results suggest that other PFT substrates, play either a positive or a negative role in plant HSR, depending on the intensity and duration of high-temperature exposure, in an ABA-independent manner. According to published literature, some members of HSP40 family are substrates of PFT. There potential roles in the HIT5-mediated HSRs is being clarified. In addition, several revertants of hit5 were isolated by screening M2 individuals of EMS-mutagenized hit5 population. These revertants will be used to identify other genetic determinants participating in HIT5 medisted plant HSRs.
關鍵字(中)	★ 阿拉伯芥 ★ 耐熱機轉 ★ 基因定位 ★ 蛋白質法尼酯化	關鍵字(英)	★ Heat-tolerance mechanism ★ Arabidospis ★ HEAT-INTOLERANT(HIT5) ★ Protein farnesylation
論文目次	中文摘要 i 英文摘要 ii 誌謝 iii 目錄 iv 圖表目錄 v 一、緒論 1 二、實驗材料與方法 6 1.實驗材料 6 2.耐熱回復突變株（Revertant 45）之位址定位 7 3.HSP40幼苗對抗離層酸（ABA）逆境測試 11 4.鹽分逆境對hit5及HSP40生長之測試 11 5.滲透壓逆境對於hit5及HSP40生長之測試 11 6.HSP40成株植物於開花週期之觀察 11 7.HSP40於高溫處理之耐熱性測試 12 8.HSP40 BiFC assay（bimolecular fluorescence complementation assay）質體建構 12 9.HSP40之雙分子螢光互補分析（BiFC assay）14 10.HSP40相關轉殖株於高溫處理之耐熱性測試 17 三、實驗結果 18 四、討論 23 五、參考文獻 30 六、附錄 30 1.hit5熱相關性狀不受ABA調節 55
參考文獻	Andrews M., Huizinga D. H., Crowell D. N. (2010). The CaaX specificities of Arabidopsis protein prenyltransferases explain era1 and ggb phenotypes. BMC plant biology, 10(1), 118. Atsmon-Raz Y., Tieleman D. P. (2017). Parameterization of palmitoylated cysteine, farnesylated cysteine, geranylgeranylated cysteine, and myristoylated glycine for the Martini force field. The Journal of Physical Chemistry B, 121(49), 11132-11143. Barghetti A., Sjögren L., Floris M., Paredes E. B., Wenkel S., Brodersen P. (2017). Heat-shock protein 40 is the key farnesylation target in meristem size control, abscisic acid signaling, and drought resistance. Genes & Development. Caplan A. J., Tsai J., Casey P. J., Douglas M. G. (1992). Farnesylation of YDJ1p is required for function at elevated growth temperatures in Saccharomyces cerevisiae. Journal of Biological Chemistry, 267(26), 18890-18895. Chang P. F. L., Lin C. Y. (2000). The discovery of the heat shock response in plants. In Discoveries In Plant Biology: Volume III (pp. 347-370). Craig E. A., Gambill B. D., Nelson R. J. (1993). Heat shock proteins: molecular chaperones of protein biogenesis. Microbiological reviews, 57(2), 402-414. Cyr D. M., Ramos C. H. (2015). Specification of Hsp70 function by type I and type II Hsp40. In The Networking of Chaperones by Co-chaperones, Springer, Cham. pp. 91-102. Dutilleul C., Ribeiro I., Blanc N., Nezames C. D., Deng X. W., Zglobicki P., Barrera A. M. P., Atehortùa L., Courtois M., Labas V., Giglioli‐Guivarc′h N. (2016). ASG2 is a farnesylated DWD protein that acts as ABA negative regulator in Arabidopsis. Plant, cell & environment, 39(1), 185-198. Galichet A., Gruissem W. (2003). Protein farnesylation in plants—conserved mechanisms but different targets. Current opinion in plant biology, 6(6), 530-535. Greer D. H., Weston C. (2010). Heat stress affects flowering, berry growth, sugar accumulation and photosynthesis of Vitis vinifera cv. Semillon grapevines grown in a controlled environment. Functional Plant Biology, 37(3), 206-214. Gurley W. B., Key J. L. (1991). Transcriptional regulation of the heat-shock response: a plant perspective. Biochemistry, 30(1), 1-12. Ham B. K., Park J. M., Lee S. B., Kim M. J., Lee I. J., Kim K. J., Kwon C. S., Paek K. H. (2006). Tobacco Tsip1, a DnaJ-type Zn finger protein, is recruited to and potentiates Tsi1-mediated transcriptional activation. The Plant Cell, 18(8), 2005-2020. Hendrick J. P., Hartl F. U. (1993). Molecular chaperone functions of heat-shock proteins. Annual review of biochemistry, 62(1), 349-384. Jiang H., Zhang X., Chen X., Aramsangtienchai, P., Tong Z., Lin H. (2018). Protein lipidation: occurrence, mechanisms, biological functions, and enabling technologies. Chemical reviews, 118(3), 919-988. Johnson C. D., Chary S. N., Chernoff E. A., Zeng Q., Running M. P., Crowell D. N. (2005). Protein geranylgeranyltransferase I is involved in specific aspects of abscisic acid and auxin signaling in Arabidopsis. Plant Physiology, 139(2), 722-733. Kimura Y., Yahara I., Lindquist S. (1995). Role of the protein chaperone YDJ1 in establishing Hsp90-mediated signal transduction pathways. Science, 268(5215), 1362-1365. Kotak S., Larkindale J., Lee U., von Koskull-Döring P., Vierling E., Scharf K. D. (2007). Complexity of the heat stress response in plants. Current Opinion in Plant biology, 10(3), 310-316. Lee J. H., Schöffl F. (1996). An Hsp70 antisense gene affects the expression of HSP70/HSC70, the regulation of HSF, and the acquisition of thermotolerance in transgenic Arabidopsis thaliana. Molecular and General genetics MGG, 252(1-2), 11-19. Lee S., Tsai F. T. (2005). Molecular chaperones in protein quality control. J Biochem Mol Biol, 38(3), 259-265. Leng L., Liang Q., Jiang J., Zhang C., Hao Y., Wang X., Su W. (2017). A subclass of HSP70s regulate development and abiotic stress responses in Arabidopsis thaliana. Journal of plant research, 130(2), 349-363. Lesk C., Rowhani P., Ramankutty N. (2016). Influence of extreme weather disasters on global crop production. Nature, 529(7584), 84. Lin B. L., Wang J. S., Liu H. C., Chen R. W., Meyer Y., Barakat A., Delseny M. (2001). Genomic analysis of the Hsp70 superfamily in Arabidopsis thaliana. Cell stress & chaperones, 6(3), 201. LIU H. C., LIAO H. T., CHARNG Y. Y. (2011). The role of class A1 heat shock factors (HSFA1s) in response to heat and other stresses in Arabidopsis. Plant, cell & environment, 34(5), 738-751. Liu H. T., Gao F., Li G. L., Han J. L., Liu D. L., Sun D. Y., Zhou R. G. (2008). The calmodulin‐binding protein kinase 3 is part of heat‐shock signal transduction in Arabidopsis thaliana. The Plant Journal, 55(5), 760-773. Mayer M. P., Bukau B. (2005). Hsp70 chaperones: cellular functions and molecular mechanism. Cellular and molecular life sciences, 62(6), 670. Neumann D., Nover L., Parthier B., Rieger R., Scharf K. D., Wollgiehn R. (1989). Heat shock and other stress response systems of plants. Results and problems in cell differentiation, 16, 1-155. Parsell D. A., Lindquist S. (1993). The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins. Annual review of genetics, 27(1), 437-496. Reindl A., Schoffl F., Schell J., Koncz C., Bako L. (1997). Phosphorylation by a cyclin-dependent kinase modulates DNA binding of the Arabidopsis heat-shock transcription factor HSF1 in vitro. Plant physiology, 115(1), 93-100. Riabowol K. T., Mizzen L. A., Welch W. J. (1988). Heat shock is lethal to fibroblasts microinjected with antibodies against hsp70. Science, 242(4877), 433-436. Running M. P. (2014). The role of lipid post–translational modification in plant developmental processes. Frontiers in plant science, 5, 50. Sable A., Agarwal S. K. (2018). Plant Heat Shock Protein Families: Essential Machinery for Development and Defense. Journal of Biological Sciences and Medicine, 4(1), 51-64. Scharf K. D., Berberich T., Ebersberger I., Nover L. (2012). The plant heat stress transcription factor (Hsf) family: structure, function and evolution. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1819(2), 104-119. Su P. H., Li H. M. (2010). Stromal Hsp70 is important for protein translocation into pea and Arabidopsis chloroplasts. The Plant Cell, tpc-109. Venne A. S., Kollipara L., Zahedi R. P. (2014). The next level of complexity: crosstalk of posttranslational modifications. Proteomics, 14(4-5), 513-524. Vierling E. (1991). The roles of heat shock proteins in plants. Annual review of plant biology, 42(1), 579-620. Wang M., Casey P. J. (2016). Protein prenylation: unique fats make their mark on biology. Nature reviews Molecular cell biology, 17(2), 110. Wang W. X., Vinocur B., Shoseyov O., Altman A. (2000). Biotechnology of plant osmotic stress tolerance physiological and molecular considerations. In IV International Symposium on In Vitro Culture and Horticultural Breeding 560 (pp. 285-292). Wang W., Vinocur B., Shoseyov O., Altman A. (2004). Role of plant heat-shock proteins and molecular chaperones in the abiotic stress response. Trends in plant science, 9(5), 244-252. Waters E. R., Lee G. J., Vierling E. (1996). Evolution, structure and function of the small heat shock proteins in plants. Journal of Experimental Botany, 47(3), 325-338. Wu J. R., Wang L. C., Lin Y. R., Weng C. P., Yeh C. H., Wu S. J. (2017). The Arabidopsis heat‐intolerant 5 (hit5)/enhanced response to aba 1 (era1) mutant reveals the crucial role of protein farnesylation in plant responses to heat stress. New Phytologist, 213(3), 1181-1193. Yamada K., Fukao Y., Hayashi M., Fukazawa M., Suzuki I., Nishimura M. (2007). Cytosolic HSP90 regulates the heat shock response that is responsible for heat acclimation in Arabidopsis thaliana. Journal of Biological Chemistry, 282(52), 37794-37804. Yoda H., Yamaguchi Y., Sano H. (2003). Induction of hypersensitive cell death by hydrogen peroxide produced through polyamine degradation in tobacco plants. Plant Physiology, 132(4), 1973-1981. Yoshida T., Ohama N., Nakajima J., Kidokoro S., Mizoi J., Nakashima K., Maruyama K., Kim J. M., Seki M., Todaka D., Osakabe Y. (2011). Arabidopsis HsfA1 transcription factors function as the main positive regulators in heat shock-responsive gene expression. Molecular Genetics and Genomics, 286(5-6), 321-332. Zhang X. P., Glaser E. (2002). Interaction of plant mitochondrial and chloroplast signal peptides with the Hsp70 molecular chaperone. Trends in plant science, 7(1), 14-21. Zhou W., Zhou T., Li M. X., Zhao C. L., Jia N., Wang X. X., Sun Y. Z., Li G. L., Xu M., Zhou R. G., Li B. (2012). The Arabidopsis J‐protein AtDjB1 facilitates thermotolerance by protecting cells against heat‐induced oxidative damage. New Phytologist, 194(2), 364-378. Zhu J. K., Bressan R. A., Hasegawa P. M. (1993). Isoprenylation of the plant molecular chaperone ANJ1 facilitates membrane association and function at high temperature. Proceedings of the National Academy of Sciences, 90(18), 8557-8561.
指導教授	吳少傑(Shaw-Jye Wu)	審核日期	2018-11-20
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