設計並建構一全氟碳光生物反應器組用於分離混合氣體中之二氧化碳並同時提升微藻養殖及其經濟產物生成之效能

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姓名

孫仁厚(Jen-Hou Sun) 查詢紙本館藏

畢業系所

生醫科學與工程學系

論文名稱

設計並建構一全氟碳光生物反應器組用於分離混合氣體中之二氧化碳並同時提升微藻養殖及其經濟產物生成之效能
(Design and Establishment of a Synthetic Perfluorocarbon Photobioreactor System for Carbon Dioxide Separation and Enhancement of Microalgal Growth and Productions)

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摘要(中)

隨著溫室效應之日益受到重視，如何降低碳排放以及去除大氣層中過高比例的CO2已成為國際間重視的環保議題。為了有效去除混合氣體中的CO2並同時達成經濟產值的提升，本研究建構出一以全氟碳化物(Perfluorocarbon；PFC)為媒介之光生物反應器組(Perfluorinated Photobioreactor System；PPBRS)。本系統利用PFC優異的CO2吸收能力進行分離混合氣體中的CO2，並將PFC所吸收到的CO2以適當比例(v/v)輸送至擬球藻生長的光生物反應器瓶中，而擬球藻行光合作用所產生出O2再藉由PFC的傳遞導入另一單元中作收集。首先在單獨給予擬球藻不同濃度CO2並研究何種濃度為最適合擬球藻生長濃度實驗中發現，2% CO2的成長優於其他濃度組別(1 - 12%)，經與對照組(純打入空氣之組別)比較後發現，10天內的擬球藻細胞濃度多了2倍，生物質(Biomass)提高了1.25倍、總脂質(Total lipid)提高了1.57倍而二十碳五烯酸(eicosapentaenoic acid，EPA)提高了1.37倍。之後在實際於PPBRS全系統操作10天中，我們以60% N2 - 40% CO2作為模擬混合氣體並將其輸入至含有PFC的氣體純化分離單元，其吸附CO2的PFC再搭配吸附N2的PFC使其含有2% (v/v)的二氧化碳比例來供給擬球藻生長。結果顯示，PPBRS能在10天的操作下，有效的分離出混合氣體中的N2並其內的CO2濃度維持在4%以下；與對照組(單純打入空氣)比較，PPBRS組內的擬球藻細胞濃度提高了2.64倍；總生物質提高了2.17倍、總脂質提高了2.92倍、EPA提高了3.08倍；而期間的氧氣收集效率約為82.7%。綜合以上所述，本研究所開發的全氟碳化物光生物反應器系統組(PPBRS)提供了一個有效分離CO2、收集O2並能同時提升擬球藻生長與其經濟作物(生物質, 總脂質, EPA)產量之技術方法。

摘要(英)

The method to reduce carbon emission and remove excessive CO2 in the atmosphere has become an international environmental issue since greenhouse effect has gained increasing attention in the world. In order to effectively remove CO2 from the mixture and simultaneously increase the economic output, a synthetic photobioreactor system, named perfluorinated photobioreactor system (PPBRS) was established in this study. The system is aimed to utilize the excellent CO2 absorption capacity of the PFC to isolate the CO2 from the mixed gas and deliver the absorbed CO2 under an appropriate ratio (v/v) to the photobioreactor for an improved microalgal growth. Furthermore, the O2 generated from the microalgae photosynthesis will be transferred through PFC adsorption and collected in another container. Our data showed that the 2% (v/v) CO2 brought by PFC may provide the highest growth rate of N. oculata compared to the ones with other settings (1 - 12% (v/v) CO2). In comparison to the cells growing with air, the cells with 2% (v/v) CO2 supply for 10 days exhibited 2-, 1.25-, 1.57-, and 1.37-fold increases of cell concentration, amounts of biomass, total lipid, and eicosapentaenoic acid (EPA), respectively. In terms of PPBRS operation for 10 days where a mixture gas of 60% N2 - 40% CO2 was employed as the model mixed gas, the results showed that the PPBRS was able to effectively isolate CO2 through PFC adsorption and maintain the CO2 concentration in the output N2 in  4% for up to 10 days. In comparison to the group with air injection throughout the time course, our data showed that the cells cultured with PPBRS provided 2.64-fold increase of cell density; 2.17-fold increase of total biomass amount; 2.92-fold increase of total lipid amount, and 3.08-fold increase of EPA production. In additional, the oxygen collection rate was about 82.7% based on the numerical evaluation. Taken together, the developed PPBRS may serve as an effective means for simultaneous CO2 separation, O2 collection, and enhanced microalgae/N. oculata productions that is highly applicable for use in the industry.

關鍵字(中)

★ 擬球藻
★ 光生物反應器
★ 全氟碳化物
★ 生物質
★ 生物脂質
★ 二氧化碳分離
★ 氧氣收集

關鍵字(英)

★ Microalgae
★ Photobioreactor
★ Perfluorocarbon
★ Biolipid
★ CO2 separation
★ oxygen collection

論文目次

誌謝 I
摘要 II
Abstract IV
圖目錄 XI
表目錄 XIII
附表目錄 XIV
第一章前言 1
1-1 研究背景 1
1-2研究動機與目的 4
第二章文獻探討 5
2-1 溫室氣體 5
2-1-1 二氧化碳於溫室效應 5
2-2 溫室氣體對環境之影響 6
2-2-1 其他溫室氣體對環境之影響 7
2-3 二氧化碳分離技術 8
2-3-1 化學吸收法 8
2-3-2 物理吸收法 8
2-3-3 化學吸附法 9
2-3-4 物理吸附法 9
2-3-5 冷凍分離法 9
2-3-6 薄膜分離法 10
2-3-7 固態化學吸收法 10
2-3-8 生物反應法 10
2-4 微藻介紹 11
2-5 擬球藻介紹 14
2-6 影響微藻生長的環境因子 16
2-6-1 二氧化碳 16
2-6-2 氮源濃度 17
2-6-3 鹽度 18
2-6-4 酸鹼值 18
2-6-5 溫度 19
2-6-6 氧氣 19
2-7 藻類收集方法 21
2-7-1 離心法 21
2-7-2 過濾法 21
2-7-3 凝絮法 22
2-8光生物反應器之分類與技術發展 23
2-8-1 反應器的設計 24
2-9 全氟碳化物 26
2-9-1 全氟碳化物細胞培養與保存技術 28
第三章實驗方法與材料 29
3-1 實驗設計 29
3-2 實驗器材與藥品 30
3-3. 韋因培養基(Walnes’ medium)成分 35
3-4 藻種來源與培養 36
3-5 擬球藻脂質之萃取 37
3-6 擬球藻脂質中EPA之含量分析 38
3-7 全氟碳化物對模擬混合氣體之純化效率分析 39
3-8 以全氟碳化物對模擬光生物反應器進行氧氣收取效率分析 39
3-9 數據和統計分析 42
第四章結果與討論 43
4-1 以不同的通氣速率對擬球藻進行培養之影響 43
4-2 探討以不同二氧化碳體積百分比濃度對擬球藻培養之影響 46
4-2-1 光生物反應器(擬球藻培養環境) 46
4-3 二氧化碳對於擬球藻總脂質及EPA產量之影響 52
4-4 FC-40純化效能測試與討論 54
4-4-1 氣體純化單元 54
4-4-2 氣體純化能力實驗結果與討論 54
4-5 氧氣收取效能測試與討論 59
4-5-1 氧氣收取系統 59
4-5-2 氧氣收取效率結果與討論 59
4-6 PPBRS同時於微藻養殖與氣體純化以及收集效能測試與討論 63
4-6-1 PPBRS對於擬球藻生長以及氣體收集效能之探討 63
4-6-2 PPBRS對於擬球藻總脂質以及EPA之影響 68
第五章結論 70
第六章未來展望 71
參考文獻 72
附錄 81

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指導教授

李宇翔(Yu-Hsiang Lee)

審核日期

2017-11-28

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