雙離子胺基酸吸附劑在血液中重金屬 吸附之應用

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姓名

方雲龍(Yun-Lung Fang) 查詢紙本館藏

畢業系所

生醫科學與工程學系

論文名稱

雙離子胺基酸吸附劑在血液中重金屬吸附之應用
(Amino acid-base hydrogel: A heavy metal ion scavenger in blood)

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摘要(中)

重金屬污染是當今最重要的環境問題之一，含銅廢水常由農業中農藥噴灑、粉刷顏料和皮革工業生產的過程中未經處理直接排放到自然環境，經由食物鏈的堆積時將嚴重影響人體的健康，例如阿爾茨海默病、帕金森病或威爾森氏症。藥物螯合被廣泛用於治療銅超載疾病，但據報導其具有破壞免疫系統、腎臟，並可能使已有神經問題的患者的神經症狀惡化…副作用等。
在先前的研究中以證明雙離子氨基酸的聚合物具有優異的生物相容性和防污性能且能夠藉由其官能基專一的螯合銅離子。在這裡，我們開發了一種基於氨基酸的水凝膠吸附劑，並將其填充至管柱方便快速過濾患者血液中的銅離子。我們在不同條件下進行了胺基酸吸附劑對Cu2 +的螯合能力，並藉由溶血試驗和血小板粘附實驗證實了血液相容性，最後通過動物實驗驗證了其去除溶液中的銅離子之效果。我們期望此氨基酸吸附劑在將來能作為Cu2+ 清除劑及在血液透析上的應用。

摘要(英)

Heavy metal pollution has been one of the most important environmental problems today. Copper-containing wastewater is extensively released from different industries. The release of metal ions into food chains results in serious healthcare issues such as overaccumulation in human body leading to Alzheimer′s, Parkinson′s and Wilson diseases. Pharmaceutical chelating agents are widely used in the treatment of copper-induced toxicity. However, it had been reported that the side effects of the treatment damage the immune system, kidneys and neurological symptoms of patients with pre-existing neurological problems.
Zwitterionic amino acid-based polymers were developed for excellent biocompatibility and antifouling properties. In previous studies, amino acid group enables specifically catching copper ions from solution by formation of a chelating structure. Here, we developed an amino acid-based hydrogel adsorbent in order to rapid and effective removal of Cu2+ ions from blood by filtration in a gel-packed column. We performed the chelating ability of gel adsorbent to Cu2 + under different conditions and confirmed the hemocompatibility by hemolysis test and platelet adhesion test. Finally, we verified the capability of gel column to remove copper ions from the solution in an animal experiment. As result, it owns good adsorption and regeneration ability, and good hemocompatibility and it also can sorption copper in blood. We expect that the amino acid-based hydrogels hold a great potential as a Cu2+-removal hemodialysis system in the future.

關鍵字(中)

★ 氨基酸
★ 水凝膠
★ 銅吸附劑
★ 兩性離子材料
★ 血液相容性
★ 透析

關鍵字(英)

★ amino acid
★ hydrogel
★ copper adsorbent
★ zwitterionic material
★ dialysis
★ hemocompatibility

論文目次

中文摘要 i
Abstract ii
目錄 iii
圖目錄 vi
表目錄 viii
第一章、研究緣起 1
第二章文獻回顧 2
2-1 金屬銅 2
2-1-1 銅在生物中的腳色 3
2-1-2 銅在體內的代謝 3
2-1-3 銅缺乏的疾病 5
2-1-4銅過量有關的疾病 6
2-2 重金屬的去除 12
2-2-1 離子交換法 12
2-2-2 膜過濾 12
2-2-3 化學沉澱 13
2-2-4 活性碳吸附劑 13
2-2-5水凝膠吸附劑 14
2-3 胺基酸啟發雙離子材料 15
第三章研究方法 18
3-1 研究內容及流程 18
3-2 實驗藥品 20
3-3 實驗方法 22
3-3-1單體合成及水凝膠合成測試 22
3-3-2 重金屬吸附實驗 26
3-3-4 動物實驗 33
第四章實驗結果與討論 34
4-1 單體合成及水凝膠測試 34
4-1-1 丙烯酰基賴氨酸 NMR 頻譜分析 34
4-1-2 單體對酸鹼之影響 34
4-1-3 膨潤率測試 36
4-2重金屬吸附實驗 37
4-2-1 重金屬吸附機制探討 37
4-2-2 重金屬吸附對吸附劑機械性質之影響 38
4-2-3 時間對吸附之影響 39
4-2-4 pH 值對吸附之影響 40
4-2-5 重金屬吸附曲線 41
4-2-6 吸附劑吸附時間改善 44
4-2-7 吸附劑之再利用性 45
4-3 相容性測試 47
4-3-1細胞毒性測試 47
4-3-2 蛋白質吸附測試 48
4-3-3 溶血測試 49
4-3-4 血小板吸附測試 50
4-4 動物實驗測試 52
第五章、結論 54
第六章、參考文獻 55

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指導教授

黃俊仁(Chun-Jen Huang)

審核日期

2018-1-26

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