化學鍍黃金電極之葡萄糖感測器製備及測試

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梁供才(Kung-Tsai Liang) 查詢紙本館藏

畢業系所

化學工程與材料工程學系

論文名稱

化學鍍黃金電極之葡萄糖感測器製備及測試

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摘要(中)

現代人因為飲食或作息不正常，造成罹患糖尿病的人逐漸變多，也增加了血糖試紙的需求量。一般黃金電極以物理濺鍍或化學氣相沉積法製成，但此方法設備成本昂貴，本研究以成本低的化學鍍方式成功製作出黃金電極的血糖試紙。利用含有鈀觸媒之油墨以網版印刷的方式，將圖形印刷在PET板子上作為基底，再透過化學鍍製程鍍上鎳、鈀和金層得到最終電極，接著滴覆上酵素層並上蓋絕緣層完成黃金電極的血糖試紙。
用製作好的黃金電極進行光電子能譜儀、附著力測試、厚度分析和電化學測試，其中電化學測試主要以循環伏安法與計時安培法進行研究，並探討酵素成份中葡萄糖氧化酶和鐵氰化鉀的組成比例，最後比較碳電極和黃金電極的差異。結果顯示以鍍鎳槽80 ℃下3-5分鐘得到0.3 - 0.5 µm，鍍鈀槽53 ℃下5分鐘得到~0.03µm厚度，鍍金槽80 ℃下8分鐘得到0.04 - 0.06µm為最佳條件的鎳-鈀-金電極，且成功放大製作在38 cm X 33 cm大小的PET基板進行得到最佳黃金血糖試紙。而酵素成份中以一倍葡萄糖氧化酶和兩倍鐵氰化鉀測試結果反應最好。最後透過比較精密度、穩定時間及添加維生素C等干擾物質，化學鍍製成的黃金電極均優於碳電極。

摘要(英)

Due to irregular diet or routine, more and more people of modern age are suffering from diabetes and increase the demand for blood glucose test strips. Gold electrodes are generally made by physical sputtering or CVD, these methods are expensive. In this study, gold electrode test strips were successfully fabricated by a low-cost electroless plating method. The PET substrate was produced by screen printing method using palladium as the catalyst. After electroless plating process, Ni, Pd and Au were plated to obtain the final electrode. The enzyme layer was dripped and the insulating layer was overlaid to complete the blood glucose test strip.
The samples were characterized by X-ray photoelectron spectroscopy, adhesion test, cyclic voltammetry and amperometric methods. The compositions of glucose oxidase and potassium ferricyanide in the enzyme components were compared. The differences between the carbon electrode and the gold electrode were compared. The results show that the optimum condition to deposit the nickel layer was 0.3 to 0.5 μm at 80 °C for 3 to 5 minutes, for palladium layer was about 0.03 μm at 53 °C for 5 minutes, and for gold layer was 0.04 to 0.06 μm at 80 °C for 8 minutes. It is successfully to manufacture on PET substrate with a size of 38 cm X 33 cm to obtain the best gold glucose test strip. The results of the test with single GOD and double potassium ferricyanide showed the best response. Finally, gold electrodes made of electroless plating are superior to carbon electrodes through comparison of precision, stability time, and addition of interfering substances such as ascorbic acid.

關鍵字(中)

★ 生物感測器
★ 黃金電極
★ 化學鍍
★ 電化學測試

關鍵字(英)

★ biosensors
★ gold electrode
★ electroless plating
★ electrochemistry test

論文目次

摘要 i
Abstract ii
目錄 iii
表目錄 vi
圖目錄 vii
第壹章緒論 1
1.1 研究背景 1
1.1.1 糖尿病簡介 2
1.1.2 血糖試紙市場 3
1.2 研究動機 3
1.3 研究目的 4
第貳章文獻回顧 5
2.1 感測器簡介 5
2.1.1 化學感測器 6
2.1.2 生物感測器 7
2.2 葡萄糖生物感測器 8
2.2.1 葡萄糖介紹 9
2.2.2 第一代葡萄糖生物感測器 9
2.2.3 第二代葡萄糖生物感測器 10
2.2.4 第三代葡萄糖生物感測器 11
2.3 化學鍍反應 12
2.3.1 化學鍍鎳 12
2.3.2 化學鍍鈀 13
2.3.3 化學鍍金 14
2.4 酵素介紹 15
2.4.1 酵素的特性 15
2.4.2 酵素的分類 16
2.4.3 葡萄糖氧化酶 (Glucose Oxidase, GOD) 17
2.5 印刷電路板製作生物感測器方法 17
2.5.1 網印法 18
2.5.2 銅箔基板之微影製程 19
2.6 電化學分析 20
2.6.1 循環伏安法 (Cyclic Voltammetry, CV) 21
2.6.2 計時安培法 (Chronopotenitometry) 22
第參章實驗方法 24
3.1 實驗材料與藥品 24
3.2 化學鍍反應槽 26
3.2.1清洗槽 27
3.2.2微蝕槽 28
3.2.3酸洗槽 28
3.2.4預浸槽 29
3.2.5活化槽 29
3.2.6還原槽 30
3.2.7化學鍍鎳槽 30
3.2.8化學鍍鈀槽 31
3.2.9化學鍍金槽 31
3.3 葡萄糖生物感測器製備 32
3.3.1 網印膠體鈀製程 32
3.3.2 銅箔基板製程 33
3.3.3 葡萄糖溶液配製 34
3.3.4 酵素滴定 35
3.3.4電化學操作方法 36
3.4 儀器介紹 38
第肆章結果與討論 43
4.1 網印膠體鈀上鍍結果 43
4.2 厚度測量 46
4.3 電化學測量 48
第伍章結論 50
參考資料 51

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指導教授

陳郁文(Yu-Wen Chen)

審核日期

2018-6-26

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