| 摘要(英) |
Breast cancer is one of the most common cancers among women worldwide and has many subtypes. Triple-negative breast cancer is the subtype with the highest mortality in breast cancer and the worst prognosis after treatment. Current treatments are radiation therapy, chemotherapy, and mastectomy, all of which can cause serious side effects. The Chinese Medicine has documented for many years in China and the paper indicates that it has low side effects and toxicity. In our study, we find an ancient book, Pu Ji Fang, which is the most complete and comprehensive. We analyze formula of Ru Shi Men from Pu Ji Fang. Using the chi-square test to screen Sheng Ma Tang (FY001W) and effectively promote triple negative breast cancer cells apoptosis. However, there are consist of monarchs, ministers, Zuo, and ambassadors in the traditional formula. This classification assist and understand the core herbs in the formula. We hope to find the core herbs so using statistics to identify four derivatives. The FY001 and derivatives were treated in the triple negative breast cancer cells to induced apoptosis by flow cytometry, and the expression of protein were observed the different concentrations of different drugs by western blot. It was found that the protein expression casepase-3, caspase-7 and PARP decreased with the increase of concentration, indicating the cells underwent apoptosis through the intrinsic pathway. In order to realize the survival of cells at low concentrations, we investigate the autophagy markers ATG5, ATG16L1, ATG7, and LC3A/B. Finding the consistent decrease in the expression level as the concentration increased indicates that the effect of autophagy was reduced. In the future, we will enrich the background information to analyze and hoping to find the core herbs. |
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