| 姓名 |
李新為(Xin-Wei Li)
查詢紙本館藏 |
畢業系所 |
系統生物與生物資訊研究所 |
| 論文名稱 |
利用生物資訊策略找出普濟方內加速傷口癒合的新配方
(New Formula from Pu-Ji-Fang to Accelerate Wound Healing Treatments by Using Bioinformatic Approaches)
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| 相關論文 | |
| 檔案 |
 [Endnote RIS 格式]
[Bibtex 格式]
 [相關文章]  [文章引用]  [完整記錄]  [館藏目錄]  至系統瀏覽論文 (2025-1-22以後開放)
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| 摘要(中) |
長期以來，中醫古書配方一直被用於治療患者。當出現外部傷口不幸被感染時，常伴隨著蜂窩性組織炎、組織化膿、傷口潰爛，近年許多文獻提到中醫外傷藥良好的治療效果，透過調控生理訊號還有中藥的活性成分，不僅能夠加速傷口癒合，也能減少疤痕的產生，所以我們採用明代最多方劑的醫書《普濟方》作為我們資料探勘來源，建立中藥方劑資料庫，用以資料探勘找出關聯性，為了方便驗證藥物預測的正確性，我們挑選方便實驗進行的外傷類藥物，建立藥物關聯性模組，接著我們整理金創門、杖瘡門、刺瘡門的資料，內容包含六卷四百九十方，使用Apriori Algorithm分析找出外傷藥的兩種配方，其中分為內服以及外敷用藥，進行細胞學實驗及爬行實驗驗證配方確實有助於成纖維母細胞生長速度。最後，我們以預測藥物混合水凝膠進行老鼠實驗，發現傷口較深的患者，以外敷用藥（74.36％）的恢復程度優於對照組（70.14％），且外敷藥物在6天內有顯著的復原情況。可知所預測的藥物配方均較於純水凝膠的實驗組有著較好的效果。最後以蘇木紫-伊紅染色結果，可以看出在外敷用藥的實驗下，傷口的表面形狀完整、復原良好，可預期我們以這樣的模組進行各大類方劑的配方預測，能夠找到更多新穎的核心藥方。 |
| 摘要(英) |
The Traditional Chinese Medicine (TCM) formula has been used to treat patients for a long time. We used the prescription of the Ming Dynasty medical book ”Pu-Ji-Fang” to establish a database of Chinese medicine prescriptions for data exploration to find out the relevance, in order to facilitate the verification of drug predictions. We selected the traumatic drugs that are convenient for experiment, and established drug-related modules. Many literatures also mentioned that the traumatic drugs of traditional Chinese medicine can not only accelerate wound healing, but also reduce the scar production. Then we organize Jin-Chuang-Phylum, Zhang-Chuang- Phylum and Chih-Chuang- Phylum data, contain 6 volumes and 410 formula, using Apriori Algorithm to identify two formulations of traumatic drugs, which were divided into oral and external application. The cytology experiment and the wound healing test proved that the traumatic formulation really contributed to the growth rate of fibroblasts. Finally, we conducted rats experiment with a predictive drug-mixed hydrogel. The external wound medicine (74.36%) has better recovery degree than control group (70.14%) for deeper wounds, and the drugs have a significant recovery within 6 days. It can be seen that the predicted drug formula has better effects than the experimental group of pure hydrogel. In the future, we will use these modules to predict the formulation of various major prescriptions, and we can find more novel core prescriptions. |
| 關鍵字(中) |
★ 普濟方
★ 傷口癒合
★ 傳統中藥 |
關鍵字(英) |
★ Pu-Ji-Fang
★ Wound Healing
★ Traditional Chinese Medicines |
| 論文目次 |
中文摘要 I
Abstract II
目錄 III
圖目錄 V
表目錄 VI
緒論 1
1-1皮膚傷口修復機制 1
1-2 中醫學的好處 1
1-3普濟方 2
1-4 方劑學用藥理論 5
1-5 生物資訊的好處 5
1-6 關聯性分析背景 6
1-7中醫古書中外傷藥物以生物資訊找出關聯性模組 6
實驗方法與材料方法 7
2-1普濟方資料 7
2-2 Apriori關聯性運算 7
2-3 Gephi繪圖 8
2-4藥物製備 9
2-5 細胞培養 11
2-6 Wound-healing assay 細胞爬行實驗 11
2-7 老鼠實驗敷藥製備 12
2.8 老鼠實驗 13
2-9 傷口面積評估 13
2.10 H&E staining 蘇木紫-伊紅染色 14
結果 15
3-1 普濟方 各門藥物特徵 15
3-1-1 建立普濟方資料庫 15
3-1-2 普濟方方劑挑選 16
3-1-3 金瘡、杖瘡、刺瘡門藥物關聯性分析結果 17
3-1-6 金瘡、杖瘡、刺瘡門 內服用藥關聯性分析結果 20
3-1-7 金瘡、杖瘡、刺瘡門 內服用藥去除礦物關聯性分析結果 22
3-1-8 金瘡、杖瘡、刺瘡門 外敷用藥關聯性分析結果 24
3-1-9 金瘡、杖瘡、刺瘡門 外敷用藥去除礦石類關聯性分析結果 26
3-2 細胞Wound Healing爬行實驗 27
3-3 大鼠實驗 28
3-3-1 大鼠實驗傷口癒合 28
3-3-2 大鼠皮膚傷口切片H&E染色 30
討論 31
參考文獻 32
補充資料 34 |
| 參考文獻 |
1. Wiechers,J. W. The barrier function of skin in relation to percutaneous absorption of drugs. Pharm. Wkbl. Sci. Ed. ;11:185-198 ,1989.
2. Qian, Hou., et al., The four-herb chinese medicine ANBP enhances wound healing and inhibits scar formation via bidirectional regulation of transformation growth factor pathway. PLOS ONE ,2014.
3. 許濟群 (2012)。方劑學。上海: 知音出版社出版。
4. MAX (2018)。Apriori演算法介紹。Max行銷誌。
5. Mathieu Bastian., et al., Gephi.org ,2008.
6. Rakesh Agrawal.,Mining Sequential Patterns. IBM Research Report ,1994
7. Rayccy (2017)。淺談社會網絡分析。Steemit。
8. Lohmann & Rauscher International GmbH & Co. KG.Suprasorb Gel ,2011.
9. Wan-Ting Yang., et al., Effects of Angelica dahurica and Rheum officinale Extracts on excisional wound healing in rats. Evidence-Based Complementary and Alternative Medicine ,2017
10. Zhao Chen., et al., Development of models for classification
of action between heat‑clearing herbs and blood‑activating stasis‑resolving herbs based on theory of traditional Chinese medicine. Chen et al. Chin Med ,2018 13:12
11. Jeong-Hun Shin., et al., Analytical research to determine the effects of the components of ONGABO on the viability of HepG2 cancer cells by using the sovereign, minister, assistant and courier principle. Journal of Pharmacopuncture ,2012.15(4):042-051
12. Le Kuai, et al,. Sheng-ji Hua-yu formula promotes diabetic wound healing of re-epithelization via Activin/Follistatin regulation. BMC Complementary and Alternative Medicine ,2018. 18:32
13. Xiaoyu Zhang,et al., Stimulation of wound healing using bioinspired hydrogels with basic fibroblast growth factor (bFGF). International Journal of Nanomedicine ,2018:13
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| 指導教授 |
蘇立仁(Li-Jen Su)
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審核日期 |
2020-1-21 |
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