基於氮化銦鎵表面增強拉曼散射的 DNA檢測

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魏黨忠(Kieu Dang Trung) 查詢紙本館藏

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光電科學與工程學系

論文名稱

基於氮化銦鎵表面增強拉曼散射的 DNA檢測
(DNA Detection by InGaN-based Surface Enhanced Raman Scattering)

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摘要(中)

表面增強拉曼散射(surface enhanced Raman scattering, SERS)可大幅提升分子的光訊號強度，已逐漸成為分子診斷的有效工具。在本研究中，我們以塗有銀 (Ag) 奈米顆粒的氮化銦鎵(InGaN)量子井，作為新型的SERS基板，並以此檢測DNA。透過優化Ag奈米粒子的尺寸和密度，我們以局部表面電漿共振 (localized surface plasmon resonance, LSPR) 和電荷轉移共振 (charge transfer resonance, CTR) 的效應來增強DNA的SERS訊號。本研究所提出的InGaN SERS基板，可檢測濃度低至10-6 M的19-mers DNA。

摘要(英)

Surface enhanced Raman scattering has gradually become an effective tool for molecular diagnosis in light of the significant intensity magnification. In this project, InGaN (QWs), coated with silver (Ag) nanoparticles are utilized as new SERS substrate for DNA detection. Optimizing the size and density of silver (Ag) nanoparticles and the roughness of the substrate, we aim to maximize the SERS signal via localized surface plasmon resonance (LSPR) and charge transfer resonance (CTR) involving the collective oscillation of electrons at the Ag surface.
Electrons trapped within the QWs serve as new source to supply the resonance charges for LSPR and CTR process, contributing to the enhancement factor of SERS. With optimizing the size and density of Ag nanoparticles, we are able to detect the 19-mers DNA with molar concentration down to 10-6 M.

關鍵字(中)

★ 表面增強拉曼散射
★ 氮化銦鎵
★ DNA
★ 銀
★ 電荷轉移共振
★ 局部表面電漿共振

關鍵字(英)

★ Surface enhanced Raman scattering
★ InGaN
★ DNA
★ Ag
★ Charge transfer resonance
★ Localized surface Plasmon scattering

論文目次

CHINESE ABSTRACT i
ENGLISH ABSTRACT ii
ADKNOWLEDMENT iii
TABLE OF CONTENTS iv
LIST OF TABLES vi
LIST OF FIGURES viii
EXPLANATION OF ABBREVIATIONS ix
Chapter 1 1
INTRODUCTION 1
1.1. Theory of surface enhanced Raman scattering (SERS) 1
1.1.1. The principle: localized surface plasmon resonance & charge transfer resonance 1
1.1.2. The nanoparticles and substrates for SERS 3
1.1.3. The advantages of nitride quantum wells 7
1.2. Deoxyribonucleic Acid (DNA) 8
1.2.1. DNA structure 9
1.2.2. Immobilization of DNA on SERS substrate 10
1.3. DNA analysis using SERS 12
1.3.1. DNA detection using indirect SERS method 12
1.3.2. DNA detection using label-free SERS method 13
Chapter 2 14
EXPERIMENT 14
2.1. Sample preparation 14
2.1.1. The quantum well structure 14
2.1.2. Metal deposition 14
2.1.3. Annealing process 14
2.2. SERS Measurement 15
Chapter 3 17
RESULTS AND DISCUSSIONS 17
3.1. Optimizing the fabrication of SERS substrate 18
3.1.1. Metal thickness 21
3.1.2. Annealing condition 23
3.2. The effect of quantum wells 26
3.3. Limit of detection and enhancement factor 29
3.4. Stability and reproducibility 31
Chapter 4 34
CONCLUSIONS AND FUTURE WORKS 34
4.1. Conclusions 34
4.2. Future works 34
REFERENCE 35

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指導教授

賴昆佑簡汎清 Le Vu Tuan Hung(Lai, Kun-Yu Chien, Fang-Ching Le Vu Tuan Hung)

審核日期

2020-1-17

推文