| 摘要(英) |
In this study, phospholipids and cholesterol were used as coating materials for liposomes to form a hollow spherical bimolecular phospholipid layer membrane. Since the cell membrane structure of an organism is also a bimolecular phospholipid layer membrane, it has good compatibility with the human body and has high biodegradability. The spherical structure can be used as a carrier for drugs and antioxidants, and can effectively protect its contents. It is widely used in medicine, food, and maintenance products. In this study, a thin film hydration method was used to prepare astaxanthin liposome solution. Astaxanthin was encapsulated in the liposome to protect it from decomposition under high temperature and light. Traditionally, chloroform, methanol and ether were used as a solvent in synthesis. In this study ethanol and natural food were used, so there is no doubt on food safety. In this study, different ratios of phospholipids and cholesterol were used to prepare liposome solutions, and each sample was placed at 4 oC and 25 oC, respectively, for 60 days. The stability test was performed on days 7, 14, 30, and 60, respectively. Ultraviolet-visible near-infrared spectrometer (UV-vis) was used to determine its concentration and coating effect, dynamic light scattering analyzer (DLS) was used to do particle size test, and optical microscope (OM) was used to observe its appearance.
Since the product is expected to be commercialized afterwards, a scale-up test was conducted. In the research, the test was firstly performed at a magnification of 5 times. The product was stored at 4 oC and 25 oC, respectively, and then did the concentration, encapsulate efficiency., particle size and appearance tests.
The results show that the samples must be stored at 4 oC, and the liposomes synthesized under the condition of 4: 1 phospholipid and cholesterol had the best coating effect (up to 92%) and the smallest particles Diameter (96.3 nm). The appearance is round orange red, which can also effectively prove that astaxanthin is coated in liposomes. In the scaled-up test, the product was similar with that at the small scale. The method developed in this study has commercial potential. |
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