Fermentation of Leuconostoc mesenteroides reduces abdominal fat accumulation in high-fat diet mice

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蒂利雅(Adelia Riezka Rahim) 查詢紙本館藏

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生醫科學與工程學系

論文名稱

(Fermentation of Leuconostoc mesenteroides reduces abdominal fat accumulation in high-fat diet mice)

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摘要(中)

腸道菌的種類影響著體內的新陳代謝，調節身體的營養吸收，可依此最終控制體重，因此腸道菌的分佈也導致肥胖及其他多種疾病形成。本次研究主要觀察從蒙古奶酪凝乳中分離出的益生菌Leuconostoc mesenteroides EH-1（LM）對高脂飲食小鼠腹部脂肪之影響。將ICR雌性小鼠分為五組：正常飲食、高脂飲食、高脂飲食+2% 葡萄糖、高脂飲食 + LM、與高脂飲食 + LM + 葡萄糖。每3天口服攝入 LM 一次，一共持續25天。與高脂飲食小鼠相比，當小鼠攝入LM後，能減少小鼠全身和腹部脂肪重量。LM還會降低高脂飲食小鼠的IL-6表現。我們的研究結果表明，高脂飲食小鼠的腹部脂肪組織中4-羥基壬烯醛4-hydroxynonenal (4HNE)和過氧化物酶體增殖物活化受體γ (peroxisome proliferator-activated receptor-γ, PPARγ) 表現增加，然而當提供LM給小鼠後，可降低HNE和PPARγ的表達。也額外發現，LM可產生電子，額外添加葡萄糖能增加電子的產生。LM產生的電子能使細菌附著在腸道表面中，進而提高細菌存活率和丁酸的產生。這些結果表明，LM將來可能作為一種潛在益生菌，治療內臟脂肪或肥胖症。不過還需要進一步研究分析，了解LM如何減少小鼠腹部脂肪。

摘要(英)

Gut microbiota profile affects the metabolism within the body. Gut microbiota is capable to regulate nutrients acquisition throughout the body and eventually bodyweight that leading to the development of several diseases, including obesity. This study aims to observe probiotic Leuconostoc mesenteroides EH-1 (LM), isolated from Mongolian cheese curd, effect in reducing belly fat in high-fat diet mice. Female ICR mice were divided into five groups, normal diet, high-fat diet (HFD), high-fat diet with 2% glucose, high-fat diet and LM administration, high-fat diet with LM and 2% glucose administration. LM was administrated by oral gavage every 3 days for 25 days. LM administration was able to reduce body weight, belly fat accumulation, IL-6 plasma level, 4HNE, and PPARγ in HFD mice. We also found that LM can produce electrons, and the addition of glucose will enhance electron production. The electron produced by LM plays a role in the bacterial attachment in the gut surface, thus increasing the survival rate and butyrate acid production. These results suggest that LM bacteria have a potential effect as a probiotic treatment against visceral fat or obesity in the future. Further analysis was needed to complete the pathway of LM bacteria in reducing mice belly fat.

關鍵字(中)

★ 內臟脂肪
★ 腸膜明串珠菌
★ 高脂飲食
★ 電子

關鍵字(英)

★ visceral fat
★ Leuconostoc mesenteroides
★ high fat diet
★ electron

論文目次

ABSTRACT……………………………………………………………………………… I
ACKNOWLEDGEMENTS…………………………………………………………….. III
TABLE OF CONTENTS……………………………………………………………….. IV
LIST OF FIGURES……………………………………………………………………... VI
ABBREVIATIONS LIST……………………………………………………………… VII
I. INTRODUCTION 1
II. LITERATURE REVIEW 4
2.1 Overweight and Obesity 4
2.2 Probiotic and Short-Chain Fatty Acid 5
2.3 Leuconostoc mesenteroides 9
III. METHODS 11
3.1 Glucose fermentation of L. mesenteroides EH-1 11
3.2 L. mesenteroides EH-1 Administration to High-Fat Diet Mice 11
3.3 High Performance Liquid Chromatography (HPLC) Analysis 12
3.4 The Enzyme-linked Immunosorbent Assay (ELISA) Analysis 12
3.5 Electricity Measurement 13
3.6 Cell Culture 14
3.7 TMN 355-treated Bacteria Administration to High-Fat Diet Mice 14
3.6 Western Blot 15
3.7 Statistical Analysis 16
IV. RESULTS AND DISCUSSION 17
4.1 Results 17
4.1.1 L. mesenteroides EH-1 Properties 17
4.1.2 Electricity Production by L. mesenteroides EH-1 18
4.1.3 Effects of L. mesenteroides EH-1 in High-fat Diet Mice 22
4.1.4 Effects of L. mesenteroides EH-1 pre-treated with TMN 355 in high-fat diet mice 25
4.1.5 Effects of L. mesenteroides EH-1 electricity production on Caco-2 cells 26
4.2 Discussion 28
V. CONCLUSION 32
REFERENCES 33

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指導教授

黃俊銘(Chun-Ming Huang)

審核日期

2020-7-15

推文