博碩士論文 107827604 詳細資訊




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姓名 杜氏潭美(Do Thi Tra My)  查詢紙本館藏   畢業系所 生醫科學與工程學系
論文名稱
(Identify and characterize the fermenting and electrogenic skin bacteria using selective prebiotics)
相關論文
★ Intelligent nature-derived coordinative hydrogel incorporated with HRP as dressing for infected wounds★ 表皮葡萄球菌在人類皮膚微生物總體對皮膚訊號與腦波訊號影響
★ 土壤微生物組體研究:藉由內生細菌誘導之高GABA含量水稻增加神經肽Y以及減輕小鼠焦慮★ Fermentation of Leuconostoc mesenteroides reduces abdominal fat accumulation in high-fat diet mice
★ 選擇性發酵引發劑(SFI)觸發表皮葡萄球菌發酵以緩解UV-B誘導的自由基生成★ BACILLUS AMYLOLIQUEFACIENS生長在高GABA含量稻米刺激膠原蛋白合成以及減緩磷酸三鈣誘導產生的皮膚搔癢
★ 人體汗水之乳酸鈉觸發人類皮膚益生菌之表皮葡萄球菌發酵及皮膚電導之應用★ 5-甲基糠醛抑制L-乳酸葡萄球菌的發酵 表皮葡萄球菌和雙乙酰產生:一種淺在的新型除臭劑靶向人體汗液中的细菌發酵
★ 甘油對於皮膚細菌和皮膚發電之影響★ 從人類皮膚微生物總體中鑑定溶解磷酸鈣的細菌
★ 皮膚表皮葡萄球菌透過發酵抑制紅色毛癬菌之研究★ 建立人類皮膚益生微生物菌組銀行
★ The study of in vitro and in vivo fermentation of bacteria in the skin microbiome★ 基於半胱氨酸的水凝膠與銅離子結合以抵抗USA300耐甲氧西林金黃色葡萄球菌作為有效的傷口敷料
★ 皮膚微生物基因組群揭示益生菌/益生質/後生元對搔癢症與紫外線輻射誘導產生的發炎反應之作用機制★ 藉由金黃色葡萄球菌增加抗體的產生來對抗痤瘡丙酸桿菌,用以增進痤瘡之治療
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摘要(中) 表皮葡萄球菌S. epidermidis是一種皮膚上常見的微生物能為宿主提供許多好處。在我們先前的研究中表明,表皮葡萄球菌ATCC 12228可以作為皮膚益生菌使甘油發酵,普遍認知此發酵反應能自然地發生在人類皮膚上,當發酵後產生短鏈脂肪酸Short-chain fatty acids(SCFA)後,藉由逆轉由UV-B輻射誘導所造成的氧化應激(Oxidative Stress),能阻止致病菌的生長,例如皮膚痤瘡桿菌Cutibacterium acnes或是金黃色葡萄球Staphylococcus aureus。然而益生菌的局部應用仍是一個爭議的話題。一直以來,我們實驗室專注研究從人類皮膚上所分離出的細菌,特別是能與皮膚共生的表皮葡萄球菌,當它作為一種益生菌,研究其可提供的效益以及細菌的生物電位活性,期望尋找一株細菌可用來替代目前FDA批准使用在臨床治療的ATCC 12228。
  我們已從人類皮膚中篩選分離出十多種表皮葡萄球菌菌株,並且發現其中的一種菌株U2,相較於ATCC 12228 而言U2擁有較高的發酵能力與較低的生物膜作用,此現象可降低細菌致病毒力。此外分析由益生質所介導的發酵作用後,能發現U2在通過細胞外電子轉移Extracellular Electron Transfer (EET)後,能釋放更多的電子作為次級代謝產物。
  除了益生菌篩選分析外,在實驗過程中發現一種潛在的益生質-椰油辛酸 Coco-caprylate (LCC),這是一種選擇性發酵誘導物Selective Fermentation Inducer (SFI),相比傳統的甘油發酵而言,LCC能更好地促進發酵過程。我們的研究表明,較好的皮膚益生菌以及最佳的SFI可有益於維護皮膚的微生物組成。
摘要(英) Staphylococcus epidermidis (S. epidermidis) in the skin microbiome provides a huge benefit to the host. Our previous studies demonstrated that S. epidermidis ATCC 12228 served as a skin probiotic that can mediate the glycerol fermentation which is known to naturally co-exists in the human skin to yield short-chain fatty acids (SCFAs) to protect against the growth of pathogenic bacteria such as Cutibacterium acnes or Staphylococcus aureus (strain USA300) and reverse the oxidative stress induced by UV-B radiation. However, the topical application of probiotic bacteria is still a debatable topic. In our study, we have been focusing on commensal bacterium especially S. epidermidis commensal strains that are isolated from human skin to study on their probiotic and electro biotic activity for clinical applications in replacement to FDA approved S. epidermidis (ATCC 12228). We have screened more than 10 different strains of S. epidermidis from human skin and found a commensal bacterium S. epidermidis (U2) which has higher fermentation and lower bio-film role in association to lower virulence than S. epidermidis ATCC 12228. Also, upon prebiotic mediated fermentation they release higher electron as their secondary metabolite via the extracellular electron transfer (EET). Besides the probiotic screening, we also figured out a potential prebiotic selective fermentation inducer (SFI) coco-caprylate (LCC) which can boost the fermentation process better than traditionally used glycerol. Our study reveals the better skin probiotic strain as well as optimum SFI for the beneficial maintenance of skin microbiome.
關鍵字(中) ★ 表皮葡萄球菌
★ 皮膚痤瘡桿菌
★ 細胞外電子轉移
★ 益生元
★ 益生菌
關鍵字(英) ★ Staphylococcus epidermidis (S. epidermidis)
★ Cutibacterium acne (C. acne)
★ Extracellular Electron Transfer (EET)
★ Prebiotic
★ Probiotic
論文目次 Abstract i
Acknowledgments ii
Table of contents iii
List of figures v
List of tables v
Abbreviation List vii
1. INTRODUCTION - 1 -
1.1. LITERATURE REVIEW - 2 -
1.1.1. Pro-prebiotic - 2 -
1.1.2. Bacterium - 3 -
1.1.3. Fermentation of bacterium - 4 -
1.1.4. Pyruvate dehydrogenase complex - 6 -
1.1.5. Phosphotransacetylase enzyme - 7 -
1.1.6. Intercellular adhesion gene cluster (ica) - 7 -
1.1.7. Electro-Fermentation (EF) - 8 -
2. RESEARCH OBJECTIVE - 9 -
3. MATERIALS AND METHODS - 10 -
3.1. Materials - 10 -
3.1.1. Apparatus or Instruments - 10 -
3.1.2. Reagents - 10 -
3.2. Methods - 11 -
3.2.1. Medium preparation - 11 -
3.2.2. Isolate and identify commensal S. epidermidis - 12 -
3.2.3. Identification of pdh, pta, and ica. - 13 -
3.2.4. Screening probiotic properties. - 14 -
3.2.5. Biofilm detection - 15 -
3.2.6. Detection of skin conductance - 15 -
3.2.7. Minimum bactericidal concentration - 16 -
3.2.8. In vitro Electricity detection. - 16 -
3.2.9. Nanopore sequencing, genome assembly, and annotation - 17 -
4. RESULT - 18 -
4.1. Isolate and identify S. epidermidis commensals - 18 -
4.3. Testing probiotic properties of S. epidermidis. - 21 -
4.4. Biofilm detection. - 22 -
4.5. S. epidermidis produced electricity - 23 -
4.6. Genomic analysis of U2 S. epidermidis. - 25 -
4.7. Identification of new prebiotic selective - 28 -
5. DISCUSSION - 35 -
6. CONCLUSION - 39 -
7. FUTURE WORK - 39 -
Reference - 40 -
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指導教授 黃俊銘(Chun-Ming Huang) 審核日期 2020-7-15
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