博碩士論文 107827605 詳細資訊



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姓名 吳氏蘭香(Ngo Thi Lan Huong)  查詢紙本館藏   畢業系所 生醫科學與工程學系
論文名稱 開發可生物降解的完全磷酸膽鹼水凝膠
(Development of Biodegradable Complete Phosphorylcholine Hydrogel)
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摘要(中) 水凝膠是一種三維聚合物結構，具有高含水量、柔軟性、柔韌性、與生物相容性等特殊性質，因此水凝膠成為一種有潛力的生醫材料。水凝膠適合應用於藥物傳遞系統、生醫感測器、組織工程支架、生物膜、人工器官、傷口癒合敷料、隱形眼鏡等。[1] 其中，在控制釋放藥物系統及植入式醫療器材的應用中，水凝膠的生物降解能力是一項重要的性質。[3] 因此，我們展示一項新型生物可降解的雙離子交聯劑，藉由合成2-[2-{2-(methacryloyloxy)ethyldimethylammonium}ethyl phosphate] 2-ethyl disulfide (MPCSS)，以提供可經由還原而降解的雙硫鍵結。經由結合2-甲基丙烯酰氧基乙基磷酰膽鹼(MPC)及MPCSS交聯劑，以形成完全磷酸膽鹼的雙離子水凝膠，而此水凝膠展現生物可相容及生物可降解的特性。我們深入探討完全磷酸膽鹼的雙離子水凝膠，了解其機械性質、生物相容性及抗汙貼附的能力。此外，我們探討MPCSS交聯劑水凝膠的重量損失，以衡量其可降解的特性。在這項研究中，我們初步探討了磷酸膽鹼水凝膠的注射應用.
摘要(英) Hydrogel, a three-dimensional polymer structure, has been discovered as a good biomaterial with specific properties, such as high water content, softness, flexibility, and biocompatibility. The hydrogels are great candidates for drug delivery systems, biosensors, tissue engineering scaffolds, physiological membranes, artificial organs, wound healing dress, and contact lenses. [1] Moreover, with applications for control drug delivery or implantable medical devices, the biodegradable property is in high demand. Hence, a novel zwitterionic degradable crosslinker, 2-[2-{2-(methacryloyloxy)ethyldimethylammonium}ethyl phosphate] 2-ethyl disulfide (MPCSS) was synthesized to provide a disulfide linkage for degradation via reduction. A full zwitterionic phosphorylcholine (PC) hydrogel, which is phospholipid-inspired, biocompatible and biodegradable, was created by combining zwitterionic monomer 2-Methacryloyloxyethyl phosphorylcholine (MPC) and MPCSS crosslinker. Herein, the mechanical, biocompatible and antifouling properties of the full zwitterionic PC hydrogels were investigated. Besides, the degradation characteristic of MPCSS-crosslinked hydrogel was evaluated through their weight loss. In this research, the injectable application of PC hydrogel is preliminarily explored.
關鍵字(中) ★ 雙離子交聯劑
★ 磷酸膽鹼
★ 水凝膠
★ 可降解		 關鍵字(英) ★ Zwitterionic crosslinker
★ phosphorylcholine
★ hydrogel
★ degradable
論文目次 ABSTRACT v
Acknowledgment vii
Table of contents vii
List of figures xi
List of tables xiii
List of Abbreviations xiv
Chapter 1: Literature Review 1
1.1. Overview of hydrogel. 1
1.2. Formation of hydrogels 1
1.3.1 Polymer for hydrogels. 1
1.3.2. Crosslinking of hydrogels 3
1.3. Zwitterionic hydrogel 4
1.4. Biodegradable hydrogel systems 6
1.4.1. Hydrolytically degraded hydrogels 6
1.4.2. Cell-mediated degradation in hydrogel systems 7
1.4.3. Degradable hydrogels based on natural polymers 9
1.4.4. Photodegradable hydrogels 10
1.5. Review of MPC 13
1.5.1. Biological Phosphorylcholine (PC) structure 13
1.5.2. Poly(phosphorylcholine) 14
1.5.3. Applications of 2-methacryloyloxyethyl phosphorylcholine (MPC) 16
Chapter 2: Research Objectives 19
Chapter 3: Materials and Methods 21
3.1. Materials 21
3.2. Methods 22
3.2.1. Synthesis of biodegradable MPCSS crosslinker 22
3.2.1.1. 2-Chloro-1,3,2-dioxaphospholane (CP) 22
3.2.1.2. 2-Chloro-2-oxo-1,3,2-dioxaphospholane (COP) 22
3.2.1.3. 2-[2-2-(Methacryloyloxy)ethyldimethylammonium}ethyl -phosphate]ethyl disulfide (MPCSS) 23
3.2.1.4. 2-(2-oxo-1,3,2-dioxaphospholane-2-yloxy)ethyl disulphide (OOPES) 23
3.2.2. Degradable property of the crosslinker 24
3.2.3 The cytotoxicity of MPCSS crosslinker. 24
3.2.4. MPC/MPCSS hydrogel preparation 24
3.2.5. The swelling ratio and mechanical characterization of the hydrogel 25
3.2.6. Protein fouling test by enzyme-linked immunosorbent assay (ELISA) 25
3.2.7. Degradability of PC hydrogels 26
3.2.8. The cytotoxicity of PC hydrogels 26
3.2.9. Cell adhesion on PC hydrogels 27
3.2.10. The cytotoxicity of degraded PC hydrogels 27
3.2.11. Injectable process of PC hydrogels 28
3.3. Characterization 28
Chapter 4: Results and Discussion 29
4.1. Synthesis MPC crosslinker. 29
4.2. Degradable property of MPCSS crosslinker 32
4.3. Evaluation of the cytotoxicity of MPCSS crosslinker 34
4.4. Complete Phosphorylcholine Hydrogel 35
4.3.1. The swelling ratio of PC Hydrogels 35
4.3.2. Mechanical properties of PC hydrogels 36
4.3.3. Degradability of PC hydrogels 38
4.3.4. Protein absorption on the PC hydrogels 39
4.3.5. Evaluation of in vitro biocompatibility of PC hydrogels 41
4.3.6. Assessment of Cell resistance of PC hydrogels in vitro 42
4.3.7. The cytotoxicity of degraded PC hydrogels 43
4.3.8. Injectable PC hydrogels 44
Chapter 5: Conclusions and Future works 46
BIBLIOGRAPHY 47
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指導教授 李宇翔 黃俊仁(Lee,Yu-Hsiang Huang,Chun-Jen) 審核日期 2021-1-18
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