利用磷脂質促進硬脂基化胜肽之基因輸送

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謝勗元(Syu-Yuan Sie) 查詢紙本館藏

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化學工程與材料工程學系

論文名稱

利用磷脂質促進硬脂基化胜肽之基因輸送
(The Use of Phospholipid to Promote Gene Delivery of Stearylated Peptide Vehicles)

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摘要(中)

細胞穿膜胜肽(CPP)能與細胞膜相互作用，可作為藥物載體並具備基因輸送的潛力。Indolicidin(IL) 是一種帶正電的CPP，先前研究中將IL之C端硬脂基化，稱為ILs，結果發現ILs擁有自組裝效果，可作為基因載體遞送質體DNA進行轉染。為提升ILs與DNA結構穩定性，本研究嘗試加入輔助性脂質DOPE，以不同莫耳比與ILs混合來製備胜肽/脂質複合物。臨界微胞濃度測試(CMC)及DLS分析證實ILs能與DOPE帶正電的微結構，而SAXS結果顯示ILs呈現六角相結構而ILs/DOPE會自組裝成單層囊泡，其形態也透過TEM進行確認。電泳膠體實驗證實，將DOPE加入ILs可提高與DNA複合效果，但肝素競爭實驗結果顯示DNA和載體的結合主要還是倚賴帶正電的ILs，因為ILs/DOPE表面上的ILs較少，少量添加DOPE(P0.3L)會降低載體與DNA的親和力。流式細胞儀及ONPG轉染分析結果顯示，P0.3L擁有最佳的細胞攝取效果及轉染效率。雷射共軛焦顯微鏡結果顯示ILs/DOPE所帶入的DNA可自內體逃脫，為了瞭解其轉染機制，分別進行了Chloroquine、Bafilomycin A1以及酸性環境下鈣黃綠素滲漏實驗，其結果表明ILs/DOPE在酸化環境下可瓦解內體膜，促進內體逃脫。這些結果顯示DOPE的添加的確可此促進ILs的基因輸送效果。

摘要(英)

Cell-penetrating peptides (CPPs) can interact with cell membranes and thus are potential carriers for gene delivery. Indolicidin (IL) is a cationic CPP. Our previous study has stearylated C-terminal of IL and denoted it as ILs. ILs can self-assemble as gene carriers to deliver plasmid DNA for transfection. In order to improve the stability of microstructure of gene carriers, we included dioleoylphosphatidylethanolamine (DOPE), a helper lipid, with ILs. Critical micelle concentration (CMC) and DLS analyses confirmed that ILs and DOPE can form cationic microstructures. The SAXS results indicated that ILs presented a hexagonal phase structure and ILs/DOPE self-assembled a monolayer vesicle, and their morphologies were confirmed by TEM. Electrophoresis experiments showed that the addition of DOPE to ILs can improve their complexation efficiency to DNA, but the results of heparin competition revealed that DNA complexation was mainly related to cationic ILs molecules. Because there were fewer ILs on the surface of ILs/DOPE, the affinity of ILs/DOPE to DNA was lower than that of the ILs group. Flow cytometry and transfection experiments indicated that P0.3L exhibited the best cell uptake and transfection efficiency. Confocal microscopy showed that DNA delivered by ILs/DOPE could escape from endosomes. The results of chloroquine, Bafilomycin A1, and calcein leakage experiments in acidic environment all indicated that ILs/DOPE disrupted endosomal membranes and promoted DNA to escape from the acidic endosome. These results all suggested that the addition of DOPE can facilitated ILs-mediated gene delivery.

關鍵字(中)

★ 細胞穿膜胜肽
★ 基因輸送
★ 非病毒載體

關鍵字(英)

★ CPP
★ Gene delivery
★ non-viral vector
★ Indolicidin

論文目次

摘要 i
Abstract ii
目錄 iv
圖目錄 vi
表目錄 viii
第一章緒論 1
1-1 研究背景 1
1-2 研究動機 2
第二章文獻回顧 3
2-1 基因治療 3
2-2 基因載體 4
2-3 細胞穿膜胜肽(Cell Penetrating Peptides, CPPs) 6
2-3-1細胞穿膜胜肽分類 8
2-3-2細胞穿膜胜肽之改質及應用 10
2-3-3 細胞穿膜胜肽與細胞膜間的機轉 13
2-3-4 Indolicidin(IL) 15
2-4 脂質(Lipids) 18
2-4-1 脂質體(Liposomes) 23
2-4-2 脂質複合物(Lipoplexs) 25
2-4-3 輔助脂質(Helper lipids) 27
2-4-4 酸鹼度敏感型脂質體(pH-sensitive liposomes) 28
2-4-5 脂質體轉染機制 31
2-5 Peptide/Lipid載體 33
第三章實驗藥品、儀器與方法 36
3-1 實驗材料 36
3-1-1 質體DNA 36
3-1-2 胜肽(Peptide) 37
3-1-3 細胞培養藥品 37
3-1-4 分析藥品 38
3-2 實驗儀器 42
3-3 實驗方法 44
3-3-1 溶液配置 44
3-3-2 胜肽/脂質(Peptide/Lipid)奈米粒子製備 48
3-3-3 載體奈米粒子/DNA複合物製備 48
3-3-4 質體DNA純化 49
3-3-5 HEK-293T細胞培養 50
3-3-6 載體及載體/DNA物性鑑定 53
3-3-7 包覆率測定 54
3-3-8 生物適合性測試 55
3-3-9 轉染效率分析 59
3-3-10 內體逃脫(endosomal escape)效應分析 63
第四章結果與討論 66
4-1 奈米粒子物性鑑定 66
4-1-1 臨界微胞濃度(Critical micelle concentration) 66
4-1-2 粒徑大小及表面電位 68
4-1-3 X光小角度散射(Small angle-X ray scattering) 71
4-1-4 穿透式電子顯微鏡(TEM) 74
4-2 載體與DNA間的穩定性 75
4-2-1 粒徑大小 75
4-2-2 表面電位 77
4-2-3 電泳測試 78
4-2-2 EtBr螢光標定法 80
4-2-3 肝素競爭實驗 81
4-3 生物適合性測試 83
4-3-1 鈣黃綠素染劑滲漏實驗(Calcein dye leakage) 83
4-3-2 MTT細胞毒性測試 85
4-4 載體細胞攝取效果 87
4-4-1 流式細胞儀分析(Flow cytometry) 87
4-4-2 轉染效率 89
4-5 內體逃脫(endosomal escape)效應探討 91
4-4-1 雷射共軛焦顯微鏡 91
4-5-2 Chloroquine效應 93
4-5-2 鈣黃綠素滲漏實驗 94
4-5-3 Bafilomycin A1效應 96
第五章結論 98
第六章參考文獻 100

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指導教授

胡威文(Wei-Wen Hu)

審核日期

2021-10-28

推文