雙離子矽氧烷共聚物以沉積法對聚二甲基矽氧烷進行生物相容性修飾

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姓名

陳涴辰(Wo-Chen Chen) 查詢紙本館藏

畢業系所

生物醫學工程研究所

論文名稱

雙離子矽氧烷共聚物以沉積法對聚二甲基矽氧烷進行生物相容性修飾
(Biocompatible modification of polydimethylsiloxane by deposition of poly(2-methacryloyloxyethyl phosphorylcholine-co-methacryloxypropyl terminated polydimethylsiloxane))

相關論文

★ Development of Functional Biointerface by Mixed Oligomeric Silatranes	★ 建立雙離子高分子修飾蛋白質技術與分析
★ DEVELOPMENT AND APPLICATIONS OF CATECHOL-FUNCTIONALIZED ZWITTERIONIC POLYMER

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至系統瀏覽論文 (2026-10-27以後開放)

摘要(中)

聚二甲基矽氧烷(Polydimethylsiloxane ,PDMS)是一種常見的的矽膠材料。由於其易於製造且具有良好的機械性質、光學透明度、透氧性及化學和生物惰性，使其適合應用於導管、人工血管、隱形眼鏡、人工晶體等接觸人體或植入人體的生醫材料上。但由於PDMS表面的疏水性質，使得蛋白質和其他分子的非特異性吸附限制了其在這些應用上的發展。兩性雙離子材料2-甲基丙烯酰氧乙基磷酸胆碱(2-methacryloyloxyethyl phosphorylcholine)具有優良的親水性、生物相容性、潤滑、抗蛋白及細菌吸附能力。本實驗透過將兩性雙離子材料MPC (2-methacryloyloxyethyl phosphorylcholine )與擁有直鏈矽氧烷的mono Methacryloxypropyl terminated polydimethylsiloxane和擁有支鏈矽氧烷mono Methacryloxypropyl functional polydimethylsiloxane分別合成出擁有直鏈與支鏈輸水端的共聚物，用浸塗與噴塗兩種不同的修飾方式沉積在PDMS上，透過共聚物上的疏水鏈段與基材的PDMS之間的疏水作用力將共聚物吸附到表面上，提升PDMS表面的親水性、潤滑性與抗汙性。第一部份我們使用核磁共振光譜儀(Nuclear Magnetic Resonance spectroscopy)對聚合物進行轉化率與化學結構的鑑定，以及測定修飾溶液的黏度與表面張力了解溶液的性質；第二部份透過水接觸角、摩擦力測試檢視浸塗與噴塗兩種不同塗佈方式的親水性、表面潤滑度與耐磨性；第三部份使用原子力顯微鏡與光學顯微鏡對修飾表面形貌進行鑑定；最後部份，特過抗細菌貼附測試檢測修飾層的抗汙性。本研究透過簡單與低毒性的製程將共聚物Poly(MPC-co-MTP)與Poly(MPC-co-MFP)修飾於PDMS表面上，增加其親水性、潤滑、抗汙與生物相容性，期許為來延伸應用於各式矽膠表面修飾。

摘要(英)

Polydimethylsiloxane (PDMS) is a silicone material that has excellent elasticity, optical transparency, chemical and biological inertness, permeability to oxygen, and ease of fabrication. Therefore, PDMS has been used for biomedical applications such as catheters, vascular grafts, contact lenses, and intraocular lenses. However, it has some problems in the development of medical applications because the hydrophobic surface of PDMS, leading to non-specific adsorption of protein. Poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) is well known as its hydrophilicity, biocompatibility, lubrication, and anti-protein adsorption. In our study, the zwitterionic copolymers, poly(2-methacryloyloxyethyl phosphorylcholine-co-methacryloxypropyl terminated polydimethylsiloxane (poly(MPC-MTP))and(2-methacryloyloxyethyl phosphorylcholine-co-methacryloxypropyl functional polydimethylsiloxane (poly(MPC-MFP)), were deposited on PDMS by dip coating and spray coating in an ambient environment, and polymers will adsorbed on the surface by hydrophobic interaction between the hydrophobic segment on the copolymer and surface of PDMS to improve its hydrophilicity and antifouling properties. The nuclear magnetic resonance was used to measure the conversion rate and identification of the chemical structure of copolymer. The surface chemical composition and elemental ratios were measured by the X-ray photoelectron spectroscopy (XPS). The surface morphology was measured by atomic force microscope (AFM) and optical microscope. The hydrophilicity was determined by sessile drop water contact angle measurements. The lubrication and, antifouling property were measured by friction test, and bacteria adhesion tests, respectively. We used simple and low toxic process for modification of PDMS by poly(MPC-co-MTP) and poly(MPC-co-MFP) to improve hydrophilicity , lubrication, anti-fouling property, oxygen permeability and biocompatibility. In future work, we will extend to various types of silicone surface modification.

關鍵字(中)

★ 雙離子材料
★ 2-甲基丙烯酰氧乙基磷酸胆碱
★ 聚二甲基矽氧烷

關鍵字(英)

★ zwitterionic material
★ 2-methacryloyloxyethyl phosphorylcholine
★ Polydimethylsiloxane

論文目次

目錄
中文摘要 i
Abstract ii
目錄 iv
圖目錄 vii
表目錄 ix
化學品名詞簡稱 x
聚合物名詞簡稱 xi
一、文獻回顧 1
1-1 聚二甲基矽氧烷(Polydimethylsiloxane, PDMS)的應用與困境 1
1-2 聚二甲基矽氧烷修飾方法 3
1-2-1 表面修飾 3
1-2-2 本體修飾 4
1-3 有機矽丙烯酸塗佈 5
1-4 光起始表面修飾 6
1-5 雙離子聚合物與雙離子材料 7
1-6 線性與分支聚合物 9
1-7 噴塗修飾 11
二、研究目的 12
三、藥品、儀器與實驗方法 14
3-1 實驗藥品 14
3-2 儀器設備 15
3-3 材料合成與製備 16
3-3-1 聚二甲基矽氧烷(PDMS)片製備 16
3-3-2 Poly(MPC-co-MTP)與Poly(MPC-co-MFP)共聚物製備 16
3-4 實驗方法 17
3-4-1 聚二甲基矽氧烷(PDMS)片浸塗修飾 17
3-4-2 聚二甲基矽氧烷(PDMS)片噴塗修飾 17
3-4-3 修飾溶液黏度測試 18
3-4-4 修飾溶液表面張力測試 18
3-4-5 水接觸角測試 19
3-4-6 水下摩擦力測定及耐磨測試 19
3-4-7 X射線光電子能譜儀 (X-ray photoelectron spectroscopy, XPS)測試 19
3-4-8 原子力顯微鏡 (Atomic Force Microscope, AFM)測試 20
3-4-9 倒立式光學顯微鏡(Inverted microscope)表面形貌觀察 20
3-4-10 抗細菌貼附測試 21
四、結果與討論 22
4-1 共聚物性質鑑定 22
4-1-1 Poly(2-methacryloyloxyethyl phosphorylcholine-co-mono methacryloxypropyl terminated polydimethylsiloxane), (poly(MPC-co-MTP)) 1H NMR 鑑定 22
4-1-2 Poly(2-methacryloyloxyethyl phosphorylcholine-co-mono methacryloxypropyl functional polydimethylsiloxane), (poly(MPC-co-MFP)) 1H NMR 鑑定 24
4-1-3 不同比例共聚物實際親疏水比例計算 25
4-1-4 修飾溶液表面張力 26
4-1-5 修飾溶液黏度 27
4-1-6 共聚物在不同溶劑比例中的溶解度測試 28
4-2 浸塗修飾功能測試 30
4-2-1 Poly(MPC-co-MTP)與Poly(MPC-co-MTP)塗佈水接觸角比較 30
4-2-2 Poly(MPC-co-MTP)與Poly(MPC-co-MTP)塗佈潤滑度與耐磨測試 32
4-2-3 表面元素測定 34
4-3 噴塗修飾功能測試 35
4-3-1 修飾液流速與氣體壓力條件測試 35
4-3-2 Poly(MPC-co-MTP)與Poly(MPC-co-MTP)噴塗次數比較與摩擦力測試 37
4-4 修飾層表面形貌 39
4-4-1 原子力顯微鏡表面形貌與粗糙度測試 39
4-4-2 光學顯微鏡表面形貌 43
4-5 綜合討論 46
4-6 修飾層傳氧率測試 48
4-7 抗細菌貼附實驗 50
五、結論 52
六、未來展望 53
七、參考文獻 54

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指導教授

黃俊仁李宇翔(Chun-Jun Huang Yu-Hsiang Lee)

審核日期

2021-10-28

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